Experiment: survival 4C, 24 hrs
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LU632_RS00950 and thiS are separated by 2 nucleotides thiS and LU632_RS00960 overlap by 17 nucleotides LU632_RS00960 and thiE overlap by 11 nucleotides thiE and thiC overlap by 4 nucleotides
LU632_RS00950: LU632_RS00950 - thiazole synthase, at 207,517 to 208,293
_RS00950
LU632_RS00955: thiS - sulfur carrier protein ThiS, at 208,296 to 208,496
thiS
LU632_RS00960: LU632_RS00960 - HesA/MoeB/ThiF family protein, at 208,480 to 209,241
_RS00960
LU632_RS00965: thiE - thiamine phosphate synthase, at 209,231 to 209,878
thiE
LU632_RS00970: thiC - phosphomethylpyrimidine synthase ThiC, at 209,875 to 211,809
thiC
Position (kb) 209
210 Strain fitness (log2 ratio) -2
-1
0
1 at 208.289 kb on - strand at 208.298 kb on - strand at 208.482 kb on - strand at 208.591 kb on - strand, within LU632_RS00960 at 208.657 kb on + strand, within LU632_RS00960 at 208.658 kb on - strand, within LU632_RS00960 at 208.892 kb on - strand, within LU632_RS00960 at 209.103 kb on - strand, within LU632_RS00960 at 209.176 kb on - strand at 209.212 kb on - strand at 209.432 kb on + strand, within thiE at 209.433 kb on - strand, within thiE at 209.433 kb on - strand, within thiE at 209.488 kb on + strand, within thiE at 209.489 kb on - strand, within thiE at 209.503 kb on + strand, within thiE at 209.504 kb on - strand, within thiE at 209.505 kb on + strand, within thiE at 209.505 kb on + strand, within thiE at 209.505 kb on + strand, within thiE at 209.506 kb on - strand, within thiE at 209.506 kb on - strand, within thiE at 209.609 kb on + strand, within thiE at 209.609 kb on + strand, within thiE at 209.630 kb on + strand, within thiE at 209.630 kb on + strand, within thiE at 209.631 kb on - strand, within thiE at 209.668 kb on - strand, within thiE at 209.668 kb on - strand, within thiE at 209.878 kb on + strand at 209.915 kb on + strand at 209.916 kb on - strand at 210.320 kb on + strand, within thiC at 210.320 kb on + strand, within thiC at 210.320 kb on + strand, within thiC at 210.321 kb on - strand, within thiC at 210.490 kb on + strand, within thiC at 210.491 kb on - strand, within thiC at 210.640 kb on + strand, within thiC at 210.648 kb on - strand, within thiC at 210.782 kb on - strand, within thiC at 210.786 kb on - strand, within thiC at 210.819 kb on - strand, within thiC at 210.819 kb on - strand, within thiC
Per-strain Table
Position Strand Gene LocusTag Fraction survival 4C, 24 hrs remove 208,289 - -1.3 208,298 - -2.3 208,482 - +1.6 208,591 - LU632_RS00960 0.15 -1.0 208,657 + LU632_RS00960 0.23 -1.3 208,658 - LU632_RS00960 0.23 -0.6 208,892 - LU632_RS00960 0.54 -2.3 209,103 - LU632_RS00960 0.82 -1.3 209,176 - -0.5 209,212 - -1.0 209,432 + thiE LU632_RS00965 0.31 -0.7 209,433 - thiE LU632_RS00965 0.31 -1.4 209,433 - thiE LU632_RS00965 0.31 -1.1 209,488 + thiE LU632_RS00965 0.40 -1.0 209,489 - thiE LU632_RS00965 0.40 -0.6 209,503 + thiE LU632_RS00965 0.42 -0.8 209,504 - thiE LU632_RS00965 0.42 -0.9 209,505 + thiE LU632_RS00965 0.42 -2.3 209,505 + thiE LU632_RS00965 0.42 -0.3 209,505 + thiE LU632_RS00965 0.42 +0.0 209,506 - thiE LU632_RS00965 0.42 -0.9 209,506 - thiE LU632_RS00965 0.42 -0.8 209,609 + thiE LU632_RS00965 0.58 -0.8 209,609 + thiE LU632_RS00965 0.58 -0.1 209,630 + thiE LU632_RS00965 0.62 -0.2 209,630 + thiE LU632_RS00965 0.62 -1.2 209,631 - thiE LU632_RS00965 0.62 +0.0 209,668 - thiE LU632_RS00965 0.67 +1.0 209,668 - thiE LU632_RS00965 0.67 -1.0 209,878 + -0.6 209,915 + -2.2 209,916 - -1.3 210,320 + thiC LU632_RS00970 0.23 -0.0 210,320 + thiC LU632_RS00970 0.23 -1.6 210,320 + thiC LU632_RS00970 0.23 -0.0 210,321 - thiC LU632_RS00970 0.23 -0.0 210,490 + thiC LU632_RS00970 0.32 -0.6 210,491 - thiC LU632_RS00970 0.32 +0.5 210,640 + thiC LU632_RS00970 0.40 -1.3 210,648 - thiC LU632_RS00970 0.40 -0.0 210,782 - thiC LU632_RS00970 0.47 +1.0 210,786 - thiC LU632_RS00970 0.47 -1.6 210,819 - thiC LU632_RS00970 0.49 +1.0 210,819 - thiC LU632_RS00970 0.49 +1.0
Or see this region's nucleotide sequence