Experiment: rich media
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LU632_RS20215 and LU632_RS20220 are separated by 36 nucleotides LU632_RS20220 and LU632_RS20225 are separated by 58 nucleotides LU632_RS20225 and LU632_RS20230 overlap by 82 nucleotides LU632_RS20230 and LU632_RS20235 are separated by 49 nucleotides LU632_RS20235 and tnpA are separated by 22 nucleotides
LU632_RS20215: LU632_RS20215 - transposase, at 3,847,911 to 3,848,592
_RS20215
LU632_RS20220: LU632_RS20220 - transposase, at 3,848,629 to 3,848,808
_RS20220
LU632_RS20225: LU632_RS20225 - IS1 family transposase, at 3,848,867 to 3,849,370
_RS20225
LU632_RS20230: LU632_RS20230 - IS1-like element transposase, at 3,849,289 to 3,849,564
_RS20230
LU632_RS20235: LU632_RS20235 - RNA-guided endonuclease TnpB family protein, at 3,849,614 to 3,850,171
_RS20235
LU632_RS20240: tnpA - IS200/IS605 family transposase, at 3,850,194 to 3,850,610
tnpA
Position (kb)
3848
3849
3850 Strain fitness (log2 ratio)
-1
0
1 at 3847.871 kb on - strand at 3847.875 kb on + strand at 3847.875 kb on + strand at 3847.875 kb on + strand at 3847.875 kb on + strand at 3847.876 kb on - strand at 3847.876 kb on - strand at 3847.876 kb on - strand at 3847.877 kb on + strand at 3847.877 kb on + strand at 3847.879 kb on + strand at 3847.879 kb on + strand at 3847.880 kb on - strand at 3847.880 kb on - strand at 3848.075 kb on - strand, within LU632_RS20215 at 3848.181 kb on - strand, within LU632_RS20215 at 3848.181 kb on - strand, within LU632_RS20215 at 3848.181 kb on - strand, within LU632_RS20215 at 3848.196 kb on + strand, within LU632_RS20215 at 3848.197 kb on - strand, within LU632_RS20215 at 3848.382 kb on + strand, within LU632_RS20215 at 3848.415 kb on + strand, within LU632_RS20215 at 3848.415 kb on + strand, within LU632_RS20215 at 3848.415 kb on + strand, within LU632_RS20215 at 3848.415 kb on + strand, within LU632_RS20215 at 3848.415 kb on + strand, within LU632_RS20215 at 3848.415 kb on + strand, within LU632_RS20215 at 3848.416 kb on - strand, within LU632_RS20215 at 3848.416 kb on - strand, within LU632_RS20215 at 3848.416 kb on - strand, within LU632_RS20215 at 3848.416 kb on - strand, within LU632_RS20215 at 3848.416 kb on - strand, within LU632_RS20215 at 3848.416 kb on - strand, within LU632_RS20215 at 3848.479 kb on - strand, within LU632_RS20215 at 3848.486 kb on - strand, within LU632_RS20215 at 3848.520 kb on - strand, within LU632_RS20215 at 3849.041 kb on + strand, within LU632_RS20225 at 3849.077 kb on - strand, within LU632_RS20225 at 3849.079 kb on - strand, within LU632_RS20225 at 3849.079 kb on - strand, within LU632_RS20225 at 3850.211 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction rich media remove 3,847,871 - +0.7 3,847,875 + -0.4 3,847,875 + -0.8 3,847,875 + +1.1 3,847,875 + +1.1 3,847,876 - -0.3 3,847,876 - +0.5 3,847,876 - -0.1 3,847,877 + +0.5 3,847,877 + +0.0 3,847,879 + +0.7 3,847,879 + -0.9 3,847,880 - +0.9 3,847,880 - +0.6 3,848,075 - LU632_RS20215 0.24 +0.6 3,848,181 - LU632_RS20215 0.40 -0.4 3,848,181 - LU632_RS20215 0.40 -0.1 3,848,181 - LU632_RS20215 0.40 -0.6 3,848,196 + LU632_RS20215 0.42 -1.3 3,848,197 - LU632_RS20215 0.42 -0.5 3,848,382 + LU632_RS20215 0.69 +0.1 3,848,415 + LU632_RS20215 0.74 +0.9 3,848,415 + LU632_RS20215 0.74 -0.2 3,848,415 + LU632_RS20215 0.74 -0.3 3,848,415 + LU632_RS20215 0.74 -0.3 3,848,415 + LU632_RS20215 0.74 +0.4 3,848,415 + LU632_RS20215 0.74 -1.1 3,848,416 - LU632_RS20215 0.74 +0.0 3,848,416 - LU632_RS20215 0.74 -0.3 3,848,416 - LU632_RS20215 0.74 -0.1 3,848,416 - LU632_RS20215 0.74 +0.1 3,848,416 - LU632_RS20215 0.74 +0.3 3,848,416 - LU632_RS20215 0.74 -0.3 3,848,479 - LU632_RS20215 0.83 -0.3 3,848,486 - LU632_RS20215 0.84 -0.3 3,848,520 - LU632_RS20215 0.89 +0.4 3,849,041 + LU632_RS20225 0.35 -0.3 3,849,077 - LU632_RS20225 0.42 -0.5 3,849,079 - LU632_RS20225 0.42 -0.5 3,849,079 - LU632_RS20225 0.42 -0.3 3,850,211 + +1.3
Or see this region's nucleotide sequence