Experiment: rich media
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ruvA and ruvC are separated by 125 nucleotides ruvC and LU632_RS13425 are separated by 31 nucleotides LU632_RS13425 and nudB are separated by 146 nucleotides nudB and aspS overlap by 1 nucleotides
LU632_RS13415: ruvA - Holliday junction branch migration protein RuvA, at 2,560,156 to 2,560,773
ruvA
LU632_RS13420: ruvC - crossover junction endodeoxyribonuclease RuvC, at 2,560,899 to 2,561,423
ruvC
LU632_RS13425: LU632_RS13425 - YebC/PmpR family DNA-binding transcriptional regulator, at 2,561,455 to 2,562,198
_RS13425
LU632_RS13430: nudB - dihydroneopterin triphosphate diphosphatase, at 2,562,345 to 2,562,779
nudB
LU632_RS13435: aspS - aspartate--tRNA ligase, at 2,562,779 to 2,564,578
aspS
Position (kb)
2561
2562
2563 Strain fitness (log2 ratio)
-1
0
1 at 2560.810 kb on - strand at 2561.457 kb on - strand at 2561.703 kb on - strand, within LU632_RS13425 at 2561.805 kb on - strand, within LU632_RS13425 at 2561.940 kb on - strand, within LU632_RS13425 at 2562.064 kb on - strand, within LU632_RS13425 at 2562.184 kb on - strand at 2562.237 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction rich media remove 2,560,810 - +0.7 2,561,457 - +1.7 2,561,703 - LU632_RS13425 0.33 +0.2 2,561,805 - LU632_RS13425 0.47 -0.3 2,561,940 - LU632_RS13425 0.65 -0.5 2,562,064 - LU632_RS13425 0.82 -1.1 2,562,184 - +0.3 2,562,237 - +0.6
Or see this region's nucleotide sequence