Experiment: rich media
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt proC and LU632_RS19000 are separated by 491 nucleotides LU632_RS19000 and LU632_RS19005 are separated by 266 nucleotides LU632_RS19005 and LU632_RS19010 are separated by 81 nucleotides LU632_RS19010 and cspA are separated by 70 nucleotides cspA and LU632_RS19020 are separated by 329 nucleotides
LU632_RS18995: proC - pyrroline-5-carboxylate reductase, at 3,585,578 to 3,586,390
proC
LU632_RS19000: LU632_RS19000 - hypothetical protein, at 3,586,882 to 3,587,091
_RS19000
LU632_RS19005: LU632_RS19005 - beta-galactosidase, at 3,587,358 to 3,590,432
_RS19005
LU632_RS19010: LU632_RS19010 - hypothetical protein, at 3,590,514 to 3,590,726
_RS19010
LU632_RS19015: cspA - RNA chaperone/antiterminator CspA, at 3,590,797 to 3,591,009
cspA
LU632_RS19020: LU632_RS19020 - MarC family NAAT transporter, at 3,591,339 to 3,592,010
_RS19020
Position (kb)
3587
3588
3589
3590
3591 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3586.358 kb on - strand at 3586.451 kb on + strand at 3586.578 kb on + strand at 3586.650 kb on + strand at 3586.654 kb on + strand at 3586.655 kb on - strand at 3586.756 kb on - strand at 3586.756 kb on - strand at 3586.787 kb on - strand at 3586.787 kb on - strand at 3586.840 kb on - strand at 3586.861 kb on - strand at 3586.873 kb on + strand at 3586.874 kb on - strand at 3586.883 kb on + strand at 3587.041 kb on + strand, within LU632_RS19000 at 3587.041 kb on + strand, within LU632_RS19000 at 3587.064 kb on + strand, within LU632_RS19000 at 3587.064 kb on + strand, within LU632_RS19000 at 3587.069 kb on - strand, within LU632_RS19000 at 3587.069 kb on - strand, within LU632_RS19000 at 3587.076 kb on + strand at 3587.236 kb on + strand at 3587.236 kb on + strand at 3587.237 kb on - strand at 3587.302 kb on + strand at 3587.337 kb on - strand at 3587.339 kb on + strand at 3587.340 kb on - strand at 3587.377 kb on + strand at 3587.377 kb on + strand at 3587.395 kb on + strand at 3587.396 kb on - strand at 3587.432 kb on + strand at 3587.433 kb on - strand at 3587.665 kb on - strand at 3587.733 kb on + strand, within LU632_RS19005 at 3587.776 kb on - strand, within LU632_RS19005 at 3587.809 kb on - strand, within LU632_RS19005 at 3587.819 kb on + strand, within LU632_RS19005 at 3587.838 kb on + strand, within LU632_RS19005 at 3587.944 kb on - strand, within LU632_RS19005 at 3587.960 kb on + strand, within LU632_RS19005 at 3587.960 kb on + strand, within LU632_RS19005 at 3587.960 kb on + strand, within LU632_RS19005 at 3587.960 kb on + strand, within LU632_RS19005 at 3587.961 kb on - strand, within LU632_RS19005 at 3587.961 kb on - strand, within LU632_RS19005 at 3588.061 kb on - strand, within LU632_RS19005 at 3588.066 kb on - strand, within LU632_RS19005 at 3588.134 kb on - strand, within LU632_RS19005 at 3588.155 kb on + strand, within LU632_RS19005 at 3588.155 kb on + strand, within LU632_RS19005 at 3588.156 kb on - strand, within LU632_RS19005 at 3588.156 kb on - strand, within LU632_RS19005 at 3588.157 kb on + strand, within LU632_RS19005 at 3588.158 kb on - strand, within LU632_RS19005 at 3588.291 kb on - strand, within LU632_RS19005 at 3588.503 kb on + strand, within LU632_RS19005 at 3588.509 kb on + strand, within LU632_RS19005 at 3588.509 kb on + strand, within LU632_RS19005 at 3588.509 kb on + strand, within LU632_RS19005 at 3588.531 kb on + strand, within LU632_RS19005 at 3588.579 kb on + strand, within LU632_RS19005 at 3588.579 kb on + strand, within LU632_RS19005 at 3588.580 kb on - strand, within LU632_RS19005 at 3588.580 kb on - strand, within LU632_RS19005 at 3588.580 kb on - strand, within LU632_RS19005 at 3588.580 kb on - strand, within LU632_RS19005 at 3588.580 kb on - strand, within LU632_RS19005 at 3588.656 kb on + strand, within LU632_RS19005 at 3588.656 kb on + strand, within LU632_RS19005 at 3588.716 kb on + strand, within LU632_RS19005 at 3588.716 kb on + strand, within LU632_RS19005 at 3588.717 kb on - strand, within LU632_RS19005 at 3588.772 kb on - strand, within LU632_RS19005 at 3588.851 kb on + strand, within LU632_RS19005 at 3588.852 kb on - strand, within LU632_RS19005 at 3588.865 kb on - strand, within LU632_RS19005 at 3588.917 kb on + strand, within LU632_RS19005 at 3588.969 kb on + strand, within LU632_RS19005 at 3589.011 kb on + strand, within LU632_RS19005 at 3589.140 kb on - strand, within LU632_RS19005 at 3589.164 kb on - strand, within LU632_RS19005 at 3589.267 kb on + strand, within LU632_RS19005 at 3589.267 kb on + strand, within LU632_RS19005 at 3589.290 kb on + strand, within LU632_RS19005 at 3589.290 kb on + strand, within LU632_RS19005 at 3589.290 kb on - strand, within LU632_RS19005 at 3589.581 kb on - strand, within LU632_RS19005 at 3589.600 kb on + strand, within LU632_RS19005 at 3589.600 kb on + strand, within LU632_RS19005 at 3589.600 kb on + strand, within LU632_RS19005 at 3589.601 kb on - strand, within LU632_RS19005 at 3589.601 kb on - strand, within LU632_RS19005 at 3589.604 kb on + strand, within LU632_RS19005 at 3589.604 kb on + strand, within LU632_RS19005 at 3589.604 kb on + strand, within LU632_RS19005 at 3589.641 kb on - strand, within LU632_RS19005 at 3589.676 kb on + strand, within LU632_RS19005 at 3589.688 kb on - strand, within LU632_RS19005 at 3589.836 kb on + strand, within LU632_RS19005 at 3589.837 kb on - strand, within LU632_RS19005 at 3589.840 kb on - strand, within LU632_RS19005 at 3589.876 kb on + strand, within LU632_RS19005 at 3589.934 kb on + strand, within LU632_RS19005 at 3590.011 kb on + strand, within LU632_RS19005 at 3590.012 kb on - strand, within LU632_RS19005 at 3590.012 kb on - strand, within LU632_RS19005 at 3590.012 kb on - strand, within LU632_RS19005 at 3590.138 kb on + strand at 3590.145 kb on + strand at 3590.146 kb on - strand at 3590.146 kb on - strand at 3590.244 kb on + strand at 3590.330 kb on + strand at 3590.331 kb on - strand at 3590.390 kb on + strand at 3590.430 kb on + strand at 3590.478 kb on - strand at 3590.499 kb on + strand at 3590.500 kb on - strand at 3590.616 kb on + strand, within LU632_RS19010 at 3590.627 kb on - strand, within LU632_RS19010 at 3590.670 kb on + strand, within LU632_RS19010 at 3590.728 kb on - strand at 3590.798 kb on + strand at 3590.983 kb on + strand, within cspA at 3590.984 kb on - strand, within cspA at 3591.030 kb on - strand at 3591.074 kb on - strand at 3591.085 kb on - strand at 3591.119 kb on - strand at 3591.160 kb on - strand at 3591.212 kb on + strand at 3591.212 kb on + strand at 3591.225 kb on + strand at 3591.242 kb on - strand at 3591.248 kb on + strand at 3591.257 kb on - strand at 3591.257 kb on - strand at 3591.269 kb on - strand at 3591.295 kb on + strand at 3591.295 kb on + strand at 3591.296 kb on - strand at 3591.345 kb on + strand at 3591.345 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction rich media remove 3,586,358 - +1.0 3,586,451 + +0.4 3,586,578 + +0.6 3,586,650 + +0.0 3,586,654 + +0.0 3,586,655 - -0.5 3,586,756 - +0.2 3,586,756 - -0.1 3,586,787 - -0.2 3,586,787 - +1.6 3,586,840 - -0.3 3,586,861 - +0.4 3,586,873 + +0.3 3,586,874 - -0.0 3,586,883 + +1.4 3,587,041 + LU632_RS19000 0.76 +1.6 3,587,041 + LU632_RS19000 0.76 +0.3 3,587,064 + LU632_RS19000 0.87 -0.2 3,587,064 + LU632_RS19000 0.87 -1.1 3,587,069 - LU632_RS19000 0.89 +0.2 3,587,069 - LU632_RS19000 0.89 +0.0 3,587,076 + +0.7 3,587,236 + -2.0 3,587,236 + +1.6 3,587,237 - +0.3 3,587,302 + +0.7 3,587,337 - -0.4 3,587,339 + -1.0 3,587,340 - +0.3 3,587,377 + +0.6 3,587,377 + -0.4 3,587,395 + -0.7 3,587,396 - +0.2 3,587,432 + -0.6 3,587,433 - +0.0 3,587,665 - -1.2 3,587,733 + LU632_RS19005 0.12 -0.4 3,587,776 - LU632_RS19005 0.14 -2.0 3,587,809 - LU632_RS19005 0.15 +0.1 3,587,819 + LU632_RS19005 0.15 +0.0 3,587,838 + LU632_RS19005 0.16 +1.2 3,587,944 - LU632_RS19005 0.19 -0.6 3,587,960 + LU632_RS19005 0.20 -0.0 3,587,960 + LU632_RS19005 0.20 +0.4 3,587,960 + LU632_RS19005 0.20 +0.4 3,587,960 + LU632_RS19005 0.20 +1.0 3,587,961 - LU632_RS19005 0.20 +0.4 3,587,961 - LU632_RS19005 0.20 +0.2 3,588,061 - LU632_RS19005 0.23 +1.6 3,588,066 - LU632_RS19005 0.23 -0.2 3,588,134 - LU632_RS19005 0.25 +0.1 3,588,155 + LU632_RS19005 0.26 -0.6 3,588,155 + LU632_RS19005 0.26 +0.0 3,588,156 - LU632_RS19005 0.26 +0.6 3,588,156 - LU632_RS19005 0.26 -0.3 3,588,157 + LU632_RS19005 0.26 -0.2 3,588,158 - LU632_RS19005 0.26 +1.8 3,588,291 - LU632_RS19005 0.30 +0.1 3,588,503 + LU632_RS19005 0.37 +0.7 3,588,509 + LU632_RS19005 0.37 +0.4 3,588,509 + LU632_RS19005 0.37 -1.0 3,588,509 + LU632_RS19005 0.37 +1.0 3,588,531 + LU632_RS19005 0.38 +1.6 3,588,579 + LU632_RS19005 0.40 -0.7 3,588,579 + LU632_RS19005 0.40 -0.7 3,588,580 - LU632_RS19005 0.40 +0.1 3,588,580 - LU632_RS19005 0.40 +1.4 3,588,580 - LU632_RS19005 0.40 -0.3 3,588,580 - LU632_RS19005 0.40 -0.2 3,588,580 - LU632_RS19005 0.40 +0.9 3,588,656 + LU632_RS19005 0.42 -0.4 3,588,656 + LU632_RS19005 0.42 +1.0 3,588,716 + LU632_RS19005 0.44 -0.1 3,588,716 + LU632_RS19005 0.44 +0.2 3,588,717 - LU632_RS19005 0.44 +1.6 3,588,772 - LU632_RS19005 0.46 +0.0 3,588,851 + LU632_RS19005 0.49 +0.3 3,588,852 - LU632_RS19005 0.49 +0.6 3,588,865 - LU632_RS19005 0.49 +0.0 3,588,917 + LU632_RS19005 0.51 -0.1 3,588,969 + LU632_RS19005 0.52 +1.2 3,589,011 + LU632_RS19005 0.54 -0.4 3,589,140 - LU632_RS19005 0.58 +1.6 3,589,164 - LU632_RS19005 0.59 +0.1 3,589,267 + LU632_RS19005 0.62 -1.0 3,589,267 + LU632_RS19005 0.62 +1.0 3,589,290 + LU632_RS19005 0.63 -0.2 3,589,290 + LU632_RS19005 0.63 +0.6 3,589,290 - LU632_RS19005 0.63 -0.7 3,589,581 - LU632_RS19005 0.72 -0.2 3,589,600 + LU632_RS19005 0.73 -1.3 3,589,600 + LU632_RS19005 0.73 +0.4 3,589,600 + LU632_RS19005 0.73 -0.7 3,589,601 - LU632_RS19005 0.73 +1.0 3,589,601 - LU632_RS19005 0.73 +0.1 3,589,604 + LU632_RS19005 0.73 -2.3 3,589,604 + LU632_RS19005 0.73 +1.3 3,589,604 + LU632_RS19005 0.73 +0.5 3,589,641 - LU632_RS19005 0.74 -0.6 3,589,676 + LU632_RS19005 0.75 +0.0 3,589,688 - LU632_RS19005 0.76 -0.1 3,589,836 + LU632_RS19005 0.81 -0.6 3,589,837 - LU632_RS19005 0.81 -0.5 3,589,840 - LU632_RS19005 0.81 +0.0 3,589,876 + LU632_RS19005 0.82 -0.4 3,589,934 + LU632_RS19005 0.84 -1.3 3,590,011 + LU632_RS19005 0.86 +2.0 3,590,012 - LU632_RS19005 0.86 +0.2 3,590,012 - LU632_RS19005 0.86 +0.8 3,590,012 - LU632_RS19005 0.86 +0.0 3,590,138 + +1.0 3,590,145 + +0.7 3,590,146 - -0.4 3,590,146 - +0.0 3,590,244 + -1.0 3,590,330 + -1.0 3,590,331 - -1.4 3,590,390 + +0.3 3,590,430 + -0.7 3,590,478 - +0.2 3,590,499 + +1.3 3,590,500 - -0.3 3,590,616 + LU632_RS19010 0.48 -0.6 3,590,627 - LU632_RS19010 0.53 +0.3 3,590,670 + LU632_RS19010 0.73 +1.0 3,590,728 - +0.4 3,590,798 + +0.0 3,590,983 + cspA LU632_RS19015 0.87 -0.3 3,590,984 - cspA LU632_RS19015 0.88 +0.0 3,591,030 - +0.8 3,591,074 - +0.0 3,591,085 - +0.0 3,591,119 - -0.3 3,591,160 - +0.7 3,591,212 + -0.6 3,591,212 + +0.0 3,591,225 + -0.4 3,591,242 - -0.2 3,591,248 + +0.6 3,591,257 - +0.1 3,591,257 - +1.6 3,591,269 - -0.6 3,591,295 + -1.3 3,591,295 + +0.3 3,591,296 - -1.6 3,591,345 + +0.0 3,591,345 - +0.6
Or see this region's nucleotide sequence