Experiment: rich media
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt purM and upp are separated by 242 nucleotides upp and LU632_RS08285 overlap by 1 nucleotides LU632_RS08285 and uraA are separated by 60 nucleotides
LU632_RS08275: purM - phosphoribosylformylglycinamidine cyclo-ligase, at 1,556,194 to 1,557,234
purM
LU632_RS08280: upp - uracil phosphoribosyltransferase, at 1,557,477 to 1,558,103
upp
LU632_RS08285: LU632_RS08285 - hypothetical protein, at 1,558,103 to 1,558,258
_RS08285
LU632_RS08290: uraA - uracil permease, at 1,558,319 to 1,559,596
uraA
Position (kb) 1557
1558
1559 Strain fitness (log2 ratio) -2
-1
0
1
2 at 1556.502 kb on + strand, within purM at 1556.550 kb on - strand, within purM at 1556.553 kb on + strand, within purM at 1556.589 kb on + strand, within purM at 1556.774 kb on + strand, within purM at 1556.774 kb on + strand, within purM at 1556.789 kb on + strand, within purM at 1556.790 kb on - strand, within purM at 1556.909 kb on + strand, within purM at 1556.909 kb on + strand, within purM at 1556.909 kb on + strand, within purM at 1556.909 kb on + strand, within purM at 1556.910 kb on - strand, within purM at 1556.910 kb on - strand, within purM at 1556.912 kb on + strand, within purM at 1556.912 kb on + strand, within purM at 1556.913 kb on - strand, within purM at 1557.157 kb on - strand at 1557.209 kb on + strand at 1557.209 kb on + strand at 1557.280 kb on - strand at 1557.295 kb on + strand at 1557.402 kb on - strand at 1557.406 kb on - strand at 1557.406 kb on - strand at 1557.406 kb on - strand at 1557.433 kb on + strand at 1557.434 kb on + strand at 1557.495 kb on - strand at 1557.515 kb on + strand at 1557.516 kb on - strand at 1557.761 kb on + strand, within upp at 1557.761 kb on + strand, within upp at 1557.762 kb on - strand, within upp at 1557.899 kb on + strand, within upp at 1558.012 kb on - strand, within upp at 1558.101 kb on + strand at 1558.107 kb on + strand at 1558.107 kb on + strand at 1558.108 kb on - strand at 1558.191 kb on - strand, within LU632_RS08285 at 1558.191 kb on - strand, within LU632_RS08285 at 1558.257 kb on - strand at 1558.257 kb on - strand at 1558.268 kb on - strand at 1558.303 kb on + strand at 1558.316 kb on - strand at 1558.392 kb on + strand at 1558.496 kb on - strand, within uraA at 1558.578 kb on - strand, within uraA at 1558.864 kb on + strand, within uraA at 1558.865 kb on - strand, within uraA at 1558.897 kb on + strand, within uraA at 1558.897 kb on + strand, within uraA at 1558.897 kb on + strand, within uraA at 1558.897 kb on + strand, within uraA at 1558.897 kb on + strand, within uraA at 1558.897 kb on + strand, within uraA at 1558.897 kb on + strand, within uraA at 1558.897 kb on + strand, within uraA at 1558.897 kb on + strand, within uraA at 1558.897 kb on + strand, within uraA at 1558.897 kb on + strand, within uraA at 1558.897 kb on + strand, within uraA at 1558.898 kb on - strand, within uraA at 1558.898 kb on - strand, within uraA at 1558.898 kb on - strand, within uraA at 1558.898 kb on - strand, within uraA at 1558.898 kb on - strand, within uraA at 1558.898 kb on - strand, within uraA at 1558.898 kb on - strand, within uraA at 1558.974 kb on - strand, within uraA at 1559.073 kb on + strand, within uraA at 1559.074 kb on - strand, within uraA
Per-strain Table
Position Strand Gene LocusTag Fraction rich media remove 1,556,502 + purM LU632_RS08275 0.30 -0.3 1,556,550 - purM LU632_RS08275 0.34 +0.0 1,556,553 + purM LU632_RS08275 0.34 +0.0 1,556,589 + purM LU632_RS08275 0.38 +0.0 1,556,774 + purM LU632_RS08275 0.56 +0.0 1,556,774 + purM LU632_RS08275 0.56 +1.0 1,556,789 + purM LU632_RS08275 0.57 +0.1 1,556,790 - purM LU632_RS08275 0.57 +0.2 1,556,909 + purM LU632_RS08275 0.69 +0.0 1,556,909 + purM LU632_RS08275 0.69 -0.3 1,556,909 + purM LU632_RS08275 0.69 +0.0 1,556,909 + purM LU632_RS08275 0.69 +1.0 1,556,910 - purM LU632_RS08275 0.69 -0.2 1,556,910 - purM LU632_RS08275 0.69 +0.4 1,556,912 + purM LU632_RS08275 0.69 -1.0 1,556,912 + purM LU632_RS08275 0.69 +0.0 1,556,913 - purM LU632_RS08275 0.69 +0.0 1,557,157 - +0.6 1,557,209 + +0.0 1,557,209 + +0.6 1,557,280 - +0.0 1,557,295 + -0.6 1,557,402 - +0.1 1,557,406 - +0.0 1,557,406 - +2.0 1,557,406 - +0.7 1,557,433 + +0.0 1,557,434 + -1.0 1,557,495 - +1.0 1,557,515 + -2.0 1,557,516 - +0.0 1,557,761 + upp LU632_RS08280 0.45 -0.6 1,557,761 + upp LU632_RS08280 0.45 -1.0 1,557,762 - upp LU632_RS08280 0.45 -0.2 1,557,899 + upp LU632_RS08280 0.67 -1.0 1,558,012 - upp LU632_RS08280 0.85 -1.0 1,558,101 + -0.1 1,558,107 + +0.0 1,558,107 + +1.6 1,558,108 - +0.6 1,558,191 - LU632_RS08285 0.56 -0.4 1,558,191 - LU632_RS08285 0.56 +0.0 1,558,257 - -1.0 1,558,257 - +1.6 1,558,268 - -1.0 1,558,303 + -1.2 1,558,316 - -0.3 1,558,392 + +0.7 1,558,496 - uraA LU632_RS08290 0.14 +0.3 1,558,578 - uraA LU632_RS08290 0.20 -0.6 1,558,864 + uraA LU632_RS08290 0.43 +0.8 1,558,865 - uraA LU632_RS08290 0.43 +0.0 1,558,897 + uraA LU632_RS08290 0.45 -1.0 1,558,897 + uraA LU632_RS08290 0.45 -1.0 1,558,897 + uraA LU632_RS08290 0.45 +0.0 1,558,897 + uraA LU632_RS08290 0.45 -0.2 1,558,897 + uraA LU632_RS08290 0.45 +0.0 1,558,897 + uraA LU632_RS08290 0.45 +1.4 1,558,897 + uraA LU632_RS08290 0.45 +1.2 1,558,897 + uraA LU632_RS08290 0.45 +0.2 1,558,897 + uraA LU632_RS08290 0.45 +1.0 1,558,897 + uraA LU632_RS08290 0.45 -0.7 1,558,897 + uraA LU632_RS08290 0.45 +0.6 1,558,897 + uraA LU632_RS08290 0.45 -0.8 1,558,898 - uraA LU632_RS08290 0.45 +0.1 1,558,898 - uraA LU632_RS08290 0.45 +0.3 1,558,898 - uraA LU632_RS08290 0.45 +0.2 1,558,898 - uraA LU632_RS08290 0.45 -0.2 1,558,898 - uraA LU632_RS08290 0.45 -1.0 1,558,898 - uraA LU632_RS08290 0.45 -2.0 1,558,898 - uraA LU632_RS08290 0.45 -1.6 1,558,974 - uraA LU632_RS08290 0.51 -1.0 1,559,073 + uraA LU632_RS08290 0.59 +0.3 1,559,074 - uraA LU632_RS08290 0.59 +0.4
Or see this region's nucleotide sequence