Experiment: rich media
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gloB and LU632_RS01085 are separated by 45 nucleotides LU632_RS01085 and rnhA overlap by 16 nucleotides rnhA and dnaQ are separated by 51 nucleotides dnaQ and LU632_RS01100 are separated by 122 nucleotides
LU632_RS01080: gloB - hydroxyacylglutathione hydrolase, at 233,072 to 233,827
gloB
LU632_RS01085: LU632_RS01085 - class I SAM-dependent methyltransferase, at 233,873 to 234,610
_RS01085
LU632_RS01090: rnhA - ribonuclease HI, at 234,595 to 235,062
rnhA
LU632_RS01095: dnaQ - DNA polymerase III subunit epsilon, at 235,114 to 235,848
dnaQ
LU632_RS01100: LU632_RS01100 - tRNA-Asp, at 235,971 to 236,047
_RS01100
Position (kb) 234
235
236 Strain fitness (log2 ratio) -2
-1
0
1 at 233.604 kb on + strand, within gloB at 233.711 kb on + strand, within gloB at 233.790 kb on + strand at 233.790 kb on + strand at 233.791 kb on - strand at 233.791 kb on - strand at 233.791 kb on - strand at 233.819 kb on - strand at 233.850 kb on - strand at 233.851 kb on + strand at 234.055 kb on + strand, within LU632_RS01085 at 234.055 kb on + strand, within LU632_RS01085 at 234.370 kb on + strand, within LU632_RS01085 at 234.370 kb on + strand, within LU632_RS01085 at 234.371 kb on - strand, within LU632_RS01085 at 234.375 kb on - strand, within LU632_RS01085 at 234.628 kb on + strand at 234.659 kb on - strand, within rnhA at 234.845 kb on + strand, within rnhA at 234.846 kb on - strand, within rnhA at 234.866 kb on - strand, within rnhA at 235.102 kb on + strand at 235.850 kb on + strand at 235.851 kb on - strand at 235.889 kb on + strand at 235.889 kb on + strand at 235.890 kb on - strand at 235.938 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction rich media remove 233,604 + gloB LU632_RS01080 0.70 -0.0 233,711 + gloB LU632_RS01080 0.85 -0.0 233,790 + -1.0 233,790 + -0.0 233,791 - +1.0 233,791 - -1.6 233,791 - -1.2 233,819 - -1.0 233,850 - -1.3 233,851 + +1.0 234,055 + LU632_RS01085 0.25 -1.0 234,055 + LU632_RS01085 0.25 -1.3 234,370 + LU632_RS01085 0.67 -1.0 234,370 + LU632_RS01085 0.67 -0.0 234,371 - LU632_RS01085 0.67 +0.6 234,375 - LU632_RS01085 0.68 -1.6 234,628 + -0.0 234,659 - rnhA LU632_RS01090 0.14 -0.6 234,845 + rnhA LU632_RS01090 0.53 -0.0 234,846 - rnhA LU632_RS01090 0.54 -2.0 234,866 - rnhA LU632_RS01090 0.58 -1.0 235,102 + -0.0 235,850 + -0.2 235,851 - -1.0 235,889 + -0.7 235,889 + +1.0 235,890 - -1.0 235,938 - -0.0
Or see this region's nucleotide sequence