Experiment: rich media
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LU632_RS00960 and thiE overlap by 11 nucleotides thiE and thiC overlap by 4 nucleotides thiC and LU632_RS00975 are separated by 311 nucleotides LU632_RS00975 and nudC are separated by 85 nucleotides
LU632_RS00960: LU632_RS00960 - HesA/MoeB/ThiF family protein, at 208,480 to 209,241
_RS00960
LU632_RS00965: thiE - thiamine phosphate synthase, at 209,231 to 209,878
thiE
LU632_RS00970: thiC - phosphomethylpyrimidine synthase ThiC, at 209,875 to 211,809
thiC
LU632_RS00975: LU632_RS00975 - Rsd/AlgQ family anti-sigma factor, at 212,121 to 212,630
_RS00975
LU632_RS00980: nudC - NAD(+) diphosphatase, at 212,716 to 213,489
nudC
Position (kb)
209
210
211
212 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 208.892 kb on - strand, within LU632_RS00960 at 209.103 kb on - strand, within LU632_RS00960 at 209.176 kb on - strand at 209.212 kb on - strand at 209.432 kb on + strand, within thiE at 209.433 kb on - strand, within thiE at 209.433 kb on - strand, within thiE at 209.488 kb on + strand, within thiE at 209.489 kb on - strand, within thiE at 209.503 kb on + strand, within thiE at 209.504 kb on - strand, within thiE at 209.505 kb on + strand, within thiE at 209.505 kb on + strand, within thiE at 209.505 kb on + strand, within thiE at 209.506 kb on - strand, within thiE at 209.506 kb on - strand, within thiE at 209.609 kb on + strand, within thiE at 209.609 kb on + strand, within thiE at 209.630 kb on + strand, within thiE at 209.630 kb on + strand, within thiE at 209.631 kb on - strand, within thiE at 209.668 kb on - strand, within thiE at 209.668 kb on - strand, within thiE at 209.878 kb on + strand at 209.915 kb on + strand at 209.916 kb on - strand at 210.320 kb on + strand, within thiC at 210.320 kb on + strand, within thiC at 210.320 kb on + strand, within thiC at 210.321 kb on - strand, within thiC at 210.490 kb on + strand, within thiC at 210.491 kb on - strand, within thiC at 210.640 kb on + strand, within thiC at 210.648 kb on - strand, within thiC at 210.782 kb on - strand, within thiC at 210.786 kb on - strand, within thiC at 210.819 kb on - strand, within thiC at 210.819 kb on - strand, within thiC at 210.915 kb on - strand, within thiC at 210.982 kb on - strand, within thiC at 211.122 kb on + strand, within thiC at 211.152 kb on + strand, within thiC at 211.164 kb on + strand, within thiC at 211.329 kb on + strand, within thiC at 211.464 kb on + strand, within thiC at 211.465 kb on - strand, within thiC at 211.465 kb on - strand, within thiC at 211.537 kb on + strand, within thiC at 211.537 kb on + strand, within thiC at 211.537 kb on + strand, within thiC at 211.583 kb on + strand, within thiC at 211.809 kb on + strand at 211.810 kb on - strand at 211.860 kb on + strand at 211.860 kb on + strand at 211.861 kb on - strand at 211.930 kb on + strand at 211.930 kb on + strand at 212.028 kb on + strand at 212.042 kb on + strand at 212.074 kb on - strand at 212.093 kb on - strand at 212.094 kb on - strand at 212.150 kb on + strand at 212.195 kb on + strand, within LU632_RS00975 at 212.195 kb on + strand, within LU632_RS00975 at 212.198 kb on + strand, within LU632_RS00975 at 212.199 kb on - strand, within LU632_RS00975 at 212.272 kb on + strand, within LU632_RS00975 at 212.273 kb on - strand, within LU632_RS00975 at 212.313 kb on + strand, within LU632_RS00975 at 212.313 kb on + strand, within LU632_RS00975 at 212.314 kb on - strand, within LU632_RS00975 at 212.353 kb on + strand, within LU632_RS00975 at 212.403 kb on - strand, within LU632_RS00975 at 212.414 kb on - strand, within LU632_RS00975 at 212.441 kb on - strand, within LU632_RS00975 at 212.466 kb on - strand, within LU632_RS00975 at 212.530 kb on + strand, within LU632_RS00975 at 212.530 kb on + strand, within LU632_RS00975 at 212.533 kb on + strand, within LU632_RS00975 at 212.533 kb on + strand, within LU632_RS00975 at 212.681 kb on - strand at 212.715 kb on + strand at 212.716 kb on - strand at 212.716 kb on - strand at 212.738 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction rich media remove 208,892 - LU632_RS00960 0.54 -1.7 209,103 - LU632_RS00960 0.82 -0.0 209,176 - -1.0 209,212 - +1.0 209,432 + thiE LU632_RS00965 0.31 -0.2 209,433 - thiE LU632_RS00965 0.31 -1.0 209,433 - thiE LU632_RS00965 0.31 -0.6 209,488 + thiE LU632_RS00965 0.40 -0.0 209,489 - thiE LU632_RS00965 0.40 +0.4 209,503 + thiE LU632_RS00965 0.42 -1.2 209,504 - thiE LU632_RS00965 0.42 -0.6 209,505 + thiE LU632_RS00965 0.42 -0.0 209,505 + thiE LU632_RS00965 0.42 -0.0 209,505 + thiE LU632_RS00965 0.42 -0.7 209,506 - thiE LU632_RS00965 0.42 -0.9 209,506 - thiE LU632_RS00965 0.42 -0.9 209,609 + thiE LU632_RS00965 0.58 -1.2 209,609 + thiE LU632_RS00965 0.58 -0.4 209,630 + thiE LU632_RS00965 0.62 -0.8 209,630 + thiE LU632_RS00965 0.62 -0.4 209,631 - thiE LU632_RS00965 0.62 +1.6 209,668 - thiE LU632_RS00965 0.67 +1.0 209,668 - thiE LU632_RS00965 0.67 -0.4 209,878 + -0.0 209,915 + -0.6 209,916 - -0.3 210,320 + thiC LU632_RS00970 0.23 +1.0 210,320 + thiC LU632_RS00970 0.23 -1.0 210,320 + thiC LU632_RS00970 0.23 -1.0 210,321 - thiC LU632_RS00970 0.23 -1.0 210,490 + thiC LU632_RS00970 0.32 -1.2 210,491 - thiC LU632_RS00970 0.32 +0.9 210,640 + thiC LU632_RS00970 0.40 -0.8 210,648 - thiC LU632_RS00970 0.40 +0.7 210,782 - thiC LU632_RS00970 0.47 -0.0 210,786 - thiC LU632_RS00970 0.47 -1.0 210,819 - thiC LU632_RS00970 0.49 +1.6 210,819 - thiC LU632_RS00970 0.49 +1.0 210,915 - thiC LU632_RS00970 0.54 -1.0 210,982 - thiC LU632_RS00970 0.57 -1.1 211,122 + thiC LU632_RS00970 0.64 -0.4 211,152 + thiC LU632_RS00970 0.66 -0.3 211,164 + thiC LU632_RS00970 0.67 -0.4 211,329 + thiC LU632_RS00970 0.75 -0.8 211,464 + thiC LU632_RS00970 0.82 -1.3 211,465 - thiC LU632_RS00970 0.82 -1.0 211,465 - thiC LU632_RS00970 0.82 -2.6 211,537 + thiC LU632_RS00970 0.86 +0.7 211,537 + thiC LU632_RS00970 0.86 -0.0 211,537 + thiC LU632_RS00970 0.86 -1.3 211,583 + thiC LU632_RS00970 0.88 -0.0 211,809 + +0.4 211,810 - -0.0 211,860 + -0.0 211,860 + -0.0 211,861 - -0.4 211,930 + +0.1 211,930 + -0.0 212,028 + -0.2 212,042 + -0.0 212,074 - -0.6 212,093 - -0.9 212,094 - +0.6 212,150 + -0.0 212,195 + LU632_RS00975 0.15 +1.0 212,195 + LU632_RS00975 0.15 +0.6 212,198 + LU632_RS00975 0.15 -1.5 212,199 - LU632_RS00975 0.15 -0.3 212,272 + LU632_RS00975 0.30 -0.1 212,273 - LU632_RS00975 0.30 -0.3 212,313 + LU632_RS00975 0.38 -1.5 212,313 + LU632_RS00975 0.38 +0.4 212,314 - LU632_RS00975 0.38 +0.1 212,353 + LU632_RS00975 0.45 -0.4 212,403 - LU632_RS00975 0.55 +1.6 212,414 - LU632_RS00975 0.57 -0.7 212,441 - LU632_RS00975 0.63 -2.0 212,466 - LU632_RS00975 0.68 -0.3 212,530 + LU632_RS00975 0.80 -0.1 212,530 + LU632_RS00975 0.80 +2.3 212,533 + LU632_RS00975 0.81 -0.6 212,533 + LU632_RS00975 0.81 +1.2 212,681 - -0.0 212,715 + +0.4 212,716 - +1.0 212,716 - -0.4 212,738 + -0.5
Or see this region's nucleotide sequence