Experiment: rich media
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt thiH and LU632_RS00950 overlap by 4 nucleotides LU632_RS00950 and thiS are separated by 2 nucleotides thiS and LU632_RS00960 overlap by 17 nucleotides LU632_RS00960 and thiE overlap by 11 nucleotides
LU632_RS00945: thiH - 2-iminoacetate synthase ThiH, at 206,390 to 207,520
thiH
LU632_RS00950: LU632_RS00950 - thiazole synthase, at 207,517 to 208,293
_RS00950
LU632_RS00955: thiS - sulfur carrier protein ThiS, at 208,296 to 208,496
thiS
LU632_RS00960: LU632_RS00960 - HesA/MoeB/ThiF family protein, at 208,480 to 209,241
_RS00960
LU632_RS00965: thiE - thiamine phosphate synthase, at 209,231 to 209,878
thiE
Position (kb) 207
208
209 Strain fitness (log2 ratio) -2
-1
0
1 at 206.525 kb on - strand, within thiH at 206.567 kb on - strand, within thiH at 206.575 kb on + strand, within thiH at 206.575 kb on + strand, within thiH at 206.639 kb on + strand, within thiH at 206.639 kb on + strand, within thiH at 206.809 kb on + strand, within thiH at 206.809 kb on + strand, within thiH at 206.809 kb on + strand, within thiH at 206.809 kb on + strand, within thiH at 206.809 kb on + strand, within thiH at 206.809 kb on + strand, within thiH at 206.810 kb on - strand, within thiH at 206.810 kb on - strand, within thiH at 206.810 kb on - strand, within thiH at 206.810 kb on - strand, within thiH at 206.810 kb on - strand, within thiH at 206.811 kb on + strand, within thiH at 206.811 kb on + strand, within thiH at 206.812 kb on - strand, within thiH at 206.812 kb on - strand, within thiH at 206.812 kb on - strand, within thiH at 206.812 kb on - strand, within thiH at 206.967 kb on + strand, within thiH at 206.967 kb on + strand, within thiH at 206.968 kb on - strand, within thiH at 206.968 kb on - strand, within thiH at 207.021 kb on + strand, within thiH at 207.022 kb on - strand, within thiH at 207.077 kb on + strand, within thiH at 207.077 kb on + strand, within thiH at 207.077 kb on + strand, within thiH at 207.077 kb on + strand, within thiH at 207.078 kb on - strand, within thiH at 207.078 kb on - strand, within thiH at 207.078 kb on - strand, within thiH at 207.102 kb on + strand, within thiH at 207.103 kb on - strand, within thiH at 207.146 kb on + strand, within thiH at 207.147 kb on - strand, within thiH at 207.214 kb on + strand, within thiH at 207.249 kb on + strand, within thiH at 207.250 kb on - strand, within thiH at 207.364 kb on - strand, within thiH at 207.387 kb on - strand, within thiH at 207.387 kb on - strand, within thiH at 207.425 kb on + strand at 207.811 kb on - strand, within LU632_RS00950 at 207.811 kb on - strand, within LU632_RS00950 at 207.832 kb on - strand, within LU632_RS00950 at 207.909 kb on - strand, within LU632_RS00950 at 207.916 kb on + strand, within LU632_RS00950 at 207.917 kb on - strand, within LU632_RS00950 at 207.917 kb on - strand, within LU632_RS00950 at 208.012 kb on - strand, within LU632_RS00950 at 208.012 kb on - strand, within LU632_RS00950 at 208.012 kb on - strand, within LU632_RS00950 at 208.092 kb on + strand, within LU632_RS00950 at 208.093 kb on - strand, within LU632_RS00950 at 208.099 kb on + strand, within LU632_RS00950 at 208.100 kb on - strand, within LU632_RS00950 at 208.100 kb on - strand, within LU632_RS00950 at 208.121 kb on - strand, within LU632_RS00950 at 208.121 kb on - strand, within LU632_RS00950 at 208.289 kb on - strand at 208.298 kb on - strand at 208.482 kb on - strand at 208.591 kb on - strand, within LU632_RS00960 at 208.657 kb on + strand, within LU632_RS00960 at 208.658 kb on - strand, within LU632_RS00960 at 208.892 kb on - strand, within LU632_RS00960 at 209.103 kb on - strand, within LU632_RS00960 at 209.176 kb on - strand at 209.212 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction rich media remove 206,525 - thiH LU632_RS00945 0.12 +0.0 206,567 - thiH LU632_RS00945 0.16 +0.6 206,575 + thiH LU632_RS00945 0.16 +1.0 206,575 + thiH LU632_RS00945 0.16 -1.2 206,639 + thiH LU632_RS00945 0.22 -0.8 206,639 + thiH LU632_RS00945 0.22 +1.3 206,809 + thiH LU632_RS00945 0.37 -0.7 206,809 + thiH LU632_RS00945 0.37 -1.0 206,809 + thiH LU632_RS00945 0.37 -1.0 206,809 + thiH LU632_RS00945 0.37 -0.7 206,809 + thiH LU632_RS00945 0.37 -1.8 206,809 + thiH LU632_RS00945 0.37 -2.0 206,810 - thiH LU632_RS00945 0.37 -0.7 206,810 - thiH LU632_RS00945 0.37 +0.0 206,810 - thiH LU632_RS00945 0.37 -1.7 206,810 - thiH LU632_RS00945 0.37 -1.8 206,810 - thiH LU632_RS00945 0.37 +0.4 206,811 + thiH LU632_RS00945 0.37 +0.3 206,811 + thiH LU632_RS00945 0.37 +0.0 206,812 - thiH LU632_RS00945 0.37 -0.7 206,812 - thiH LU632_RS00945 0.37 -1.0 206,812 - thiH LU632_RS00945 0.37 -1.0 206,812 - thiH LU632_RS00945 0.37 -1.1 206,967 + thiH LU632_RS00945 0.51 -1.0 206,967 + thiH LU632_RS00945 0.51 +0.0 206,968 - thiH LU632_RS00945 0.51 -0.2 206,968 - thiH LU632_RS00945 0.51 -0.5 207,021 + thiH LU632_RS00945 0.56 +0.1 207,022 - thiH LU632_RS00945 0.56 -0.8 207,077 + thiH LU632_RS00945 0.61 -2.3 207,077 + thiH LU632_RS00945 0.61 -1.4 207,077 + thiH LU632_RS00945 0.61 +0.0 207,077 + thiH LU632_RS00945 0.61 +0.4 207,078 - thiH LU632_RS00945 0.61 +0.0 207,078 - thiH LU632_RS00945 0.61 +0.3 207,078 - thiH LU632_RS00945 0.61 +0.0 207,102 + thiH LU632_RS00945 0.63 +0.2 207,103 - thiH LU632_RS00945 0.63 -1.3 207,146 + thiH LU632_RS00945 0.67 -1.3 207,147 - thiH LU632_RS00945 0.67 -0.3 207,214 + thiH LU632_RS00945 0.73 +0.2 207,249 + thiH LU632_RS00945 0.76 -1.0 207,250 - thiH LU632_RS00945 0.76 -0.3 207,364 - thiH LU632_RS00945 0.86 -0.5 207,387 - thiH LU632_RS00945 0.88 -2.0 207,387 - thiH LU632_RS00945 0.88 -1.3 207,425 + -1.3 207,811 - LU632_RS00950 0.38 -0.2 207,811 - LU632_RS00950 0.38 -0.8 207,832 - LU632_RS00950 0.41 +0.0 207,909 - LU632_RS00950 0.50 -0.3 207,916 + LU632_RS00950 0.51 +0.0 207,917 - LU632_RS00950 0.51 -1.0 207,917 - LU632_RS00950 0.51 -1.0 208,012 - LU632_RS00950 0.64 +0.6 208,012 - LU632_RS00950 0.64 +0.3 208,012 - LU632_RS00950 0.64 -2.0 208,092 + LU632_RS00950 0.74 -0.7 208,093 - LU632_RS00950 0.74 -1.6 208,099 + LU632_RS00950 0.75 -1.0 208,100 - LU632_RS00950 0.75 -0.2 208,100 - LU632_RS00950 0.75 -0.5 208,121 - LU632_RS00950 0.78 -2.0 208,121 - LU632_RS00950 0.78 -0.7 208,289 - -1.3 208,298 - -2.3 208,482 - +0.6 208,591 - LU632_RS00960 0.15 +0.6 208,657 + LU632_RS00960 0.23 -1.1 208,658 - LU632_RS00960 0.23 +0.7 208,892 - LU632_RS00960 0.54 -1.7 209,103 - LU632_RS00960 0.82 -0.0 209,176 - -1.0 209,212 - +1.0
Or see this region's nucleotide sequence