Experiment: SMSA no antibiotic control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS03525 and RPSI07_RS24325 are separated by 329 nucleotides RPSI07_RS24325 and RPSI07_RS03535 are separated by 115 nucleotides
RPSI07_RS03525: RPSI07_RS03525 - sodium transporter, at 958,081 to 958,869
_RS03525
RPSI07_RS24325: RPSI07_RS24325 - hypothetical protein, at 959,199 to 959,930
_RS24325
RPSI07_RS03535: RPSI07_RS03535 - methyl-accepting chemotaxis protein, at 960,046 to 961,602
_RS03535
Position (kb) 959
960 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 958.303 kb on - strand, within RPSI07_RS03525 at 958.303 kb on - strand, within RPSI07_RS03525 at 958.312 kb on + strand, within RPSI07_RS03525 at 958.486 kb on + strand, within RPSI07_RS03525 at 958.486 kb on + strand, within RPSI07_RS03525 at 958.486 kb on + strand, within RPSI07_RS03525 at 958.486 kb on + strand, within RPSI07_RS03525 at 958.487 kb on - strand, within RPSI07_RS03525 at 958.487 kb on - strand, within RPSI07_RS03525 at 958.487 kb on - strand, within RPSI07_RS03525 at 958.487 kb on - strand, within RPSI07_RS03525 at 958.574 kb on + strand, within RPSI07_RS03525 at 958.575 kb on - strand, within RPSI07_RS03525 at 958.672 kb on + strand, within RPSI07_RS03525 at 958.672 kb on + strand, within RPSI07_RS03525 at 958.672 kb on + strand, within RPSI07_RS03525 at 958.673 kb on - strand, within RPSI07_RS03525 at 958.673 kb on - strand, within RPSI07_RS03525 at 958.673 kb on - strand, within RPSI07_RS03525 at 958.673 kb on - strand, within RPSI07_RS03525 at 958.673 kb on - strand, within RPSI07_RS03525 at 958.673 kb on - strand, within RPSI07_RS03525 at 958.717 kb on + strand, within RPSI07_RS03525 at 958.717 kb on + strand, within RPSI07_RS03525 at 958.718 kb on - strand, within RPSI07_RS03525 at 958.718 kb on - strand, within RPSI07_RS03525 at 958.752 kb on + strand, within RPSI07_RS03525 at 958.753 kb on - strand, within RPSI07_RS03525 at 958.755 kb on - strand, within RPSI07_RS03525 at 958.759 kb on + strand, within RPSI07_RS03525 at 958.951 kb on + strand at 958.952 kb on + strand at 958.952 kb on + strand at 958.952 kb on + strand at 958.952 kb on + strand at 958.952 kb on + strand at 958.952 kb on + strand at 958.952 kb on + strand at 958.952 kb on + strand at 958.953 kb on - strand at 958.953 kb on - strand at 958.953 kb on - strand at 958.953 kb on - strand at 958.953 kb on - strand at 958.953 kb on - strand at 958.953 kb on - strand at 958.953 kb on - strand at 958.953 kb on - strand at 958.953 kb on - strand at 958.953 kb on - strand at 958.953 kb on - strand at 958.953 kb on - strand at 958.953 kb on - strand at 958.953 kb on - strand at 958.953 kb on - strand at 958.953 kb on - strand at 958.953 kb on - strand at 959.097 kb on + strand at 959.097 kb on + strand at 959.097 kb on + strand at 959.098 kb on - strand at 959.098 kb on - strand at 959.098 kb on - strand at 959.098 kb on - strand at 959.098 kb on - strand at 959.098 kb on - strand at 959.513 kb on + strand, within RPSI07_RS24325 at 959.513 kb on + strand, within RPSI07_RS24325 at 959.513 kb on + strand, within RPSI07_RS24325 at 959.672 kb on + strand, within RPSI07_RS24325 at 959.673 kb on - strand, within RPSI07_RS24325 at 959.673 kb on - strand, within RPSI07_RS24325 at 959.673 kb on - strand, within RPSI07_RS24325 at 959.673 kb on - strand, within RPSI07_RS24325 at 959.826 kb on - strand, within RPSI07_RS24325 at 959.941 kb on + strand at 959.942 kb on - strand at 959.954 kb on + strand at 959.955 kb on - strand at 959.965 kb on + strand at 959.966 kb on - strand at 959.966 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA no antibiotic control remove 958,303 - RPSI07_RS03525 0.28 +1.4 958,303 - RPSI07_RS03525 0.28 -0.8 958,312 + RPSI07_RS03525 0.29 +1.5 958,486 + RPSI07_RS03525 0.51 -1.4 958,486 + RPSI07_RS03525 0.51 -1.1 958,486 + RPSI07_RS03525 0.51 +0.4 958,486 + RPSI07_RS03525 0.51 -0.1 958,487 - RPSI07_RS03525 0.51 +1.2 958,487 - RPSI07_RS03525 0.51 -0.6 958,487 - RPSI07_RS03525 0.51 +0.2 958,487 - RPSI07_RS03525 0.51 +0.9 958,574 + RPSI07_RS03525 0.62 -0.3 958,575 - RPSI07_RS03525 0.63 -0.5 958,672 + RPSI07_RS03525 0.75 +0.7 958,672 + RPSI07_RS03525 0.75 -1.3 958,672 + RPSI07_RS03525 0.75 +0.9 958,673 - RPSI07_RS03525 0.75 +0.3 958,673 - RPSI07_RS03525 0.75 -0.2 958,673 - RPSI07_RS03525 0.75 +0.9 958,673 - RPSI07_RS03525 0.75 +0.8 958,673 - RPSI07_RS03525 0.75 +0.2 958,673 - RPSI07_RS03525 0.75 -0.8 958,717 + RPSI07_RS03525 0.81 -1.1 958,717 + RPSI07_RS03525 0.81 -0.8 958,718 - RPSI07_RS03525 0.81 +0.5 958,718 - RPSI07_RS03525 0.81 -0.5 958,752 + RPSI07_RS03525 0.85 +0.3 958,753 - RPSI07_RS03525 0.85 +2.0 958,755 - RPSI07_RS03525 0.85 -2.4 958,759 + RPSI07_RS03525 0.86 +0.6 958,951 + -1.7 958,952 + -0.3 958,952 + -0.3 958,952 + -0.9 958,952 + -0.6 958,952 + -0.2 958,952 + +0.8 958,952 + +0.4 958,952 + -1.1 958,953 - +1.9 958,953 - -1.5 958,953 - +1.1 958,953 - -1.1 958,953 - +0.2 958,953 - -0.6 958,953 - -0.9 958,953 - -0.6 958,953 - -3.1 958,953 - -0.1 958,953 - -0.9 958,953 - -0.1 958,953 - +0.6 958,953 - +0.7 958,953 - +2.3 958,953 - +1.2 958,953 - -0.0 958,953 - -0.9 959,097 + +0.6 959,097 + -0.1 959,097 + +0.6 959,098 - -0.1 959,098 - -0.1 959,098 - +0.0 959,098 - -0.7 959,098 - -0.4 959,098 - +0.4 959,513 + RPSI07_RS24325 0.43 -1.2 959,513 + RPSI07_RS24325 0.43 -0.6 959,513 + RPSI07_RS24325 0.43 -1.1 959,672 + RPSI07_RS24325 0.65 +0.4 959,673 - RPSI07_RS24325 0.65 +1.2 959,673 - RPSI07_RS24325 0.65 -0.7 959,673 - RPSI07_RS24325 0.65 +0.2 959,673 - RPSI07_RS24325 0.65 +0.1 959,826 - RPSI07_RS24325 0.86 -0.1 959,941 + -0.0 959,942 - +0.4 959,954 + +0.7 959,955 - -0.5 959,965 + +0.5 959,966 - -0.2 959,966 - -0.5
Or see this region's nucleotide sequence