Experiment: SMSA no antibiotic control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS15590 and RPSI07_RS15595 are separated by 12 nucleotides RPSI07_RS15595 and RPSI07_RS15600 overlap by 4 nucleotides RPSI07_RS15600 and RPSI07_RS15605 are separated by 76 nucleotides
RPSI07_RS15590: RPSI07_RS15590 - FAD-binding oxidoreductase, at 1,659,710 to 1,661,122
_RS15590
RPSI07_RS15595: RPSI07_RS15595 - DUF2069 domain-containing protein, at 1,661,135 to 1,661,527
_RS15595
RPSI07_RS15600: RPSI07_RS15600 - NAD(P)H:quinone oxidoreductase, at 1,661,524 to 1,662,117
_RS15600
RPSI07_RS15605: RPSI07_RS15605 - membrane protein, at 1,662,194 to 1,663,516
_RS15605
Position (kb)
1661
1662 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1660.278 kb on + strand, within RPSI07_RS15590 at 1660.278 kb on + strand, within RPSI07_RS15590 at 1660.278 kb on + strand, within RPSI07_RS15590 at 1660.278 kb on + strand, within RPSI07_RS15590 at 1660.278 kb on + strand, within RPSI07_RS15590 at 1660.279 kb on - strand, within RPSI07_RS15590 at 1660.279 kb on - strand, within RPSI07_RS15590 at 1660.279 kb on - strand, within RPSI07_RS15590 at 1660.279 kb on - strand, within RPSI07_RS15590 at 1660.279 kb on - strand, within RPSI07_RS15590 at 1660.279 kb on - strand, within RPSI07_RS15590 at 1660.617 kb on - strand, within RPSI07_RS15590 at 1660.617 kb on - strand, within RPSI07_RS15590 at 1660.647 kb on + strand, within RPSI07_RS15590 at 1660.648 kb on - strand, within RPSI07_RS15590 at 1661.188 kb on - strand, within RPSI07_RS15595 at 1661.188 kb on - strand, within RPSI07_RS15595 at 1661.188 kb on - strand, within RPSI07_RS15595 at 1661.643 kb on - strand, within RPSI07_RS15600 at 1661.687 kb on + strand, within RPSI07_RS15600 at 1661.687 kb on + strand, within RPSI07_RS15600 at 1661.701 kb on + strand, within RPSI07_RS15600 at 1661.701 kb on + strand, within RPSI07_RS15600 at 1661.701 kb on + strand, within RPSI07_RS15600 at 1661.701 kb on + strand, within RPSI07_RS15600 at 1661.702 kb on - strand, within RPSI07_RS15600 at 1661.702 kb on - strand, within RPSI07_RS15600 at 1661.852 kb on + strand, within RPSI07_RS15600 at 1661.852 kb on + strand, within RPSI07_RS15600 at 1661.852 kb on + strand, within RPSI07_RS15600 at 1661.852 kb on - strand, within RPSI07_RS15600 at 1661.940 kb on - strand, within RPSI07_RS15600 at 1661.983 kb on + strand, within RPSI07_RS15600 at 1661.983 kb on + strand, within RPSI07_RS15600 at 1661.983 kb on + strand, within RPSI07_RS15600 at 1661.983 kb on + strand, within RPSI07_RS15600 at 1661.984 kb on - strand, within RPSI07_RS15600 at 1661.984 kb on - strand, within RPSI07_RS15600 at 1662.092 kb on + strand at 1662.092 kb on + strand at 1662.092 kb on + strand at 1662.092 kb on + strand at 1662.093 kb on - strand at 1662.093 kb on - strand at 1662.093 kb on - strand at 1662.095 kb on + strand at 1662.095 kb on + strand at 1662.096 kb on - strand at 1662.096 kb on - strand at 1662.096 kb on - strand at 1662.096 kb on - strand at 1662.096 kb on - strand at 1662.096 kb on - strand at 1662.145 kb on + strand at 1662.146 kb on - strand at 1662.146 kb on - strand at 1662.147 kb on + strand at 1662.147 kb on + strand at 1662.147 kb on + strand at 1662.147 kb on + strand at 1662.147 kb on + strand at 1662.148 kb on - strand at 1662.413 kb on + strand, within RPSI07_RS15605 at 1662.413 kb on + strand, within RPSI07_RS15605 at 1662.413 kb on + strand, within RPSI07_RS15605 at 1662.465 kb on - strand, within RPSI07_RS15605
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA no antibiotic control remove 1,660,278 + RPSI07_RS15590 0.40 +0.7 1,660,278 + RPSI07_RS15590 0.40 +0.8 1,660,278 + RPSI07_RS15590 0.40 -0.8 1,660,278 + RPSI07_RS15590 0.40 -1.1 1,660,278 + RPSI07_RS15590 0.40 -1.1 1,660,279 - RPSI07_RS15590 0.40 +0.8 1,660,279 - RPSI07_RS15590 0.40 +0.9 1,660,279 - RPSI07_RS15590 0.40 +1.1 1,660,279 - RPSI07_RS15590 0.40 -1.6 1,660,279 - RPSI07_RS15590 0.40 -0.5 1,660,279 - RPSI07_RS15590 0.40 +1.3 1,660,617 - RPSI07_RS15590 0.64 +0.2 1,660,617 - RPSI07_RS15590 0.64 -1.8 1,660,647 + RPSI07_RS15590 0.66 -1.3 1,660,648 - RPSI07_RS15590 0.66 -0.1 1,661,188 - RPSI07_RS15595 0.13 +0.3 1,661,188 - RPSI07_RS15595 0.13 -0.6 1,661,188 - RPSI07_RS15595 0.13 +0.2 1,661,643 - RPSI07_RS15600 0.20 -2.8 1,661,687 + RPSI07_RS15600 0.27 +0.0 1,661,687 + RPSI07_RS15600 0.27 -2.1 1,661,701 + RPSI07_RS15600 0.30 -0.7 1,661,701 + RPSI07_RS15600 0.30 -0.6 1,661,701 + RPSI07_RS15600 0.30 -2.0 1,661,701 + RPSI07_RS15600 0.30 -0.5 1,661,702 - RPSI07_RS15600 0.30 -0.6 1,661,702 - RPSI07_RS15600 0.30 -0.4 1,661,852 + RPSI07_RS15600 0.55 -1.9 1,661,852 + RPSI07_RS15600 0.55 -1.7 1,661,852 + RPSI07_RS15600 0.55 -1.5 1,661,852 - RPSI07_RS15600 0.55 +0.6 1,661,940 - RPSI07_RS15600 0.70 +3.1 1,661,983 + RPSI07_RS15600 0.77 -0.5 1,661,983 + RPSI07_RS15600 0.77 +0.7 1,661,983 + RPSI07_RS15600 0.77 -0.9 1,661,983 + RPSI07_RS15600 0.77 -0.2 1,661,984 - RPSI07_RS15600 0.77 +0.8 1,661,984 - RPSI07_RS15600 0.77 -1.0 1,662,092 + -0.2 1,662,092 + +0.5 1,662,092 + -0.5 1,662,092 + -1.3 1,662,093 - +1.0 1,662,093 - +1.8 1,662,093 - +0.8 1,662,095 + +1.3 1,662,095 + +2.7 1,662,096 - -0.8 1,662,096 - -1.1 1,662,096 - -0.5 1,662,096 - -0.2 1,662,096 - +0.4 1,662,096 - +0.2 1,662,145 + +0.0 1,662,146 - -1.9 1,662,146 - +0.6 1,662,147 + +0.1 1,662,147 + +0.2 1,662,147 + +1.8 1,662,147 + +0.1 1,662,147 + +2.1 1,662,148 - +0.5 1,662,413 + RPSI07_RS15605 0.17 +0.1 1,662,413 + RPSI07_RS15605 0.17 +0.1 1,662,413 + RPSI07_RS15605 0.17 +1.4 1,662,465 - RPSI07_RS15605 0.20 +0.6
Or see this region's nucleotide sequence