Experiment: SMSA no antibiotic control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS13910 and RPSI07_RS13915 overlap by 11 nucleotides RPSI07_RS13915 and RPSI07_RS13920 overlap by 8 nucleotides RPSI07_RS13920 and RPSI07_RS13925 are separated by 1 nucleotides
RPSI07_RS13910: RPSI07_RS13910 - hypothetical protein, at 1,302,728 to 1,303,222
_RS13910
RPSI07_RS13915: RPSI07_RS13915 - hypothetical protein, at 1,303,212 to 1,303,928
_RS13915
RPSI07_RS13920: RPSI07_RS13920 - type II/IV secretion system protein, at 1,303,921 to 1,305,546
_RS13920
RPSI07_RS13925: RPSI07_RS13925 - type II secretion system F family protein, at 1,305,548 to 1,306,741
_RS13925
Position (kb)
1303
1304
1305
1306 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1303.033 kb on + strand, within RPSI07_RS13910 at 1303.459 kb on + strand, within RPSI07_RS13915 at 1303.460 kb on - strand, within RPSI07_RS13915 at 1303.544 kb on - strand, within RPSI07_RS13915 at 1303.544 kb on - strand, within RPSI07_RS13915 at 1303.741 kb on + strand, within RPSI07_RS13915 at 1303.742 kb on - strand, within RPSI07_RS13915 at 1303.918 kb on + strand at 1303.919 kb on - strand at 1303.919 kb on - strand at 1303.919 kb on - strand at 1304.115 kb on + strand, within RPSI07_RS13920 at 1304.136 kb on - strand, within RPSI07_RS13920 at 1304.259 kb on - strand, within RPSI07_RS13920 at 1304.501 kb on + strand, within RPSI07_RS13920 at 1305.016 kb on + strand, within RPSI07_RS13920 at 1305.016 kb on + strand, within RPSI07_RS13920 at 1305.020 kb on - strand, within RPSI07_RS13920 at 1305.037 kb on + strand, within RPSI07_RS13920 at 1305.038 kb on - strand, within RPSI07_RS13920 at 1305.038 kb on - strand, within RPSI07_RS13920 at 1305.038 kb on - strand, within RPSI07_RS13920 at 1305.546 kb on + strand at 1305.547 kb on - strand at 1305.547 kb on - strand at 1305.547 kb on - strand at 1305.574 kb on + strand at 1305.582 kb on + strand at 1305.582 kb on + strand at 1305.582 kb on + strand at 1305.582 kb on + strand at 1305.582 kb on + strand at 1305.582 kb on + strand at 1305.582 kb on + strand at 1305.582 kb on + strand at 1305.583 kb on - strand at 1305.583 kb on - strand at 1305.583 kb on - strand at 1305.583 kb on - strand at 1305.583 kb on - strand at 1305.687 kb on + strand, within RPSI07_RS13925 at 1305.687 kb on + strand, within RPSI07_RS13925 at 1305.688 kb on - strand, within RPSI07_RS13925 at 1305.906 kb on + strand, within RPSI07_RS13925 at 1305.986 kb on + strand, within RPSI07_RS13925 at 1305.987 kb on - strand, within RPSI07_RS13925 at 1306.164 kb on + strand, within RPSI07_RS13925 at 1306.165 kb on - strand, within RPSI07_RS13925 at 1306.191 kb on + strand, within RPSI07_RS13925 at 1306.191 kb on + strand, within RPSI07_RS13925 at 1306.191 kb on + strand, within RPSI07_RS13925 at 1306.191 kb on + strand, within RPSI07_RS13925 at 1306.192 kb on - strand, within RPSI07_RS13925 at 1306.192 kb on - strand, within RPSI07_RS13925 at 1306.209 kb on - strand, within RPSI07_RS13925 at 1306.209 kb on - strand, within RPSI07_RS13925 at 1306.305 kb on + strand, within RPSI07_RS13925 at 1306.438 kb on - strand, within RPSI07_RS13925 at 1306.450 kb on - strand, within RPSI07_RS13925
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA no antibiotic control remove 1,303,033 + RPSI07_RS13910 0.62 -1.9 1,303,459 + RPSI07_RS13915 0.34 -0.9 1,303,460 - RPSI07_RS13915 0.35 -0.5 1,303,544 - RPSI07_RS13915 0.46 -0.2 1,303,544 - RPSI07_RS13915 0.46 -0.8 1,303,741 + RPSI07_RS13915 0.74 +2.9 1,303,742 - RPSI07_RS13915 0.74 -0.3 1,303,918 + +1.2 1,303,919 - -1.2 1,303,919 - -0.4 1,303,919 - -0.3 1,304,115 + RPSI07_RS13920 0.12 +0.5 1,304,136 - RPSI07_RS13920 0.13 +0.0 1,304,259 - RPSI07_RS13920 0.21 -0.4 1,304,501 + RPSI07_RS13920 0.36 -0.8 1,305,016 + RPSI07_RS13920 0.67 -0.6 1,305,016 + RPSI07_RS13920 0.67 -0.5 1,305,020 - RPSI07_RS13920 0.68 -0.1 1,305,037 + RPSI07_RS13920 0.69 +2.2 1,305,038 - RPSI07_RS13920 0.69 -1.5 1,305,038 - RPSI07_RS13920 0.69 -0.6 1,305,038 - RPSI07_RS13920 0.69 +0.4 1,305,546 + -0.6 1,305,547 - -0.5 1,305,547 - -1.1 1,305,547 - +0.0 1,305,574 + -0.7 1,305,582 + -0.6 1,305,582 + -0.2 1,305,582 + +0.0 1,305,582 + -0.9 1,305,582 + -0.5 1,305,582 + +0.8 1,305,582 + -0.6 1,305,582 + -0.7 1,305,583 - -0.5 1,305,583 - -0.1 1,305,583 - +1.1 1,305,583 - +0.1 1,305,583 - -0.8 1,305,687 + RPSI07_RS13925 0.12 -1.3 1,305,687 + RPSI07_RS13925 0.12 +0.6 1,305,688 - RPSI07_RS13925 0.12 +2.2 1,305,906 + RPSI07_RS13925 0.30 -0.5 1,305,986 + RPSI07_RS13925 0.37 -0.5 1,305,987 - RPSI07_RS13925 0.37 -0.2 1,306,164 + RPSI07_RS13925 0.52 +1.1 1,306,165 - RPSI07_RS13925 0.52 -1.5 1,306,191 + RPSI07_RS13925 0.54 -0.1 1,306,191 + RPSI07_RS13925 0.54 -0.9 1,306,191 + RPSI07_RS13925 0.54 -1.8 1,306,191 + RPSI07_RS13925 0.54 +0.5 1,306,192 - RPSI07_RS13925 0.54 +2.6 1,306,192 - RPSI07_RS13925 0.54 -1.4 1,306,209 - RPSI07_RS13925 0.55 +0.1 1,306,209 - RPSI07_RS13925 0.55 -0.3 1,306,305 + RPSI07_RS13925 0.63 +0.7 1,306,438 - RPSI07_RS13925 0.75 -0.7 1,306,450 - RPSI07_RS13925 0.76 +0.5
Or see this region's nucleotide sequence