Experiment: SMSA no antibiotic control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS13190 and RPSI07_RS13195 are separated by 96 nucleotides RPSI07_RS13195 and RPSI07_RS13200 are separated by 35 nucleotides RPSI07_RS13200 and RPSI07_RS13205 are separated by 115 nucleotides
RPSI07_RS13190: RPSI07_RS13190 - DNA translocase FtsK, at 1,144,145 to 1,146,487
_RS13190
RPSI07_RS13195: RPSI07_RS13195 - outer membrane lipoprotein carrier protein LolA, at 1,146,584 to 1,147,231
_RS13195
RPSI07_RS13200: RPSI07_RS13200 - hypothetical protein, at 1,147,267 to 1,147,683
_RS13200
RPSI07_RS13205: RPSI07_RS13205 - replication-associated recombination protein A, at 1,147,799 to 1,149,109
_RS13205
Position (kb)
1146
1147
1148 Strain fitness (log2 ratio)
-1
0
1
2
3
4
5
6 at 1145.655 kb on - strand, within RPSI07_RS13190 at 1146.176 kb on + strand, within RPSI07_RS13190 at 1146.177 kb on - strand, within RPSI07_RS13190 at 1146.203 kb on - strand, within RPSI07_RS13190 at 1146.287 kb on + strand at 1146.287 kb on + strand at 1146.287 kb on + strand at 1146.288 kb on - strand at 1146.288 kb on - strand at 1146.288 kb on - strand at 1146.366 kb on - strand at 1146.366 kb on - strand at 1146.366 kb on - strand at 1146.504 kb on + strand at 1146.515 kb on - strand at 1146.583 kb on + strand at 1146.583 kb on + strand at 1146.583 kb on + strand at 1146.827 kb on + strand, within RPSI07_RS13195 at 1146.875 kb on + strand, within RPSI07_RS13195 at 1146.875 kb on + strand, within RPSI07_RS13195 at 1146.875 kb on + strand, within RPSI07_RS13195 at 1146.876 kb on - strand, within RPSI07_RS13195 at 1146.876 kb on - strand, within RPSI07_RS13195 at 1146.882 kb on - strand, within RPSI07_RS13195 at 1146.882 kb on - strand, within RPSI07_RS13195 at 1146.882 kb on - strand, within RPSI07_RS13195
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA no antibiotic control remove 1,145,655 - RPSI07_RS13190 0.64 +1.1 1,146,176 + RPSI07_RS13190 0.87 -0.5 1,146,177 - RPSI07_RS13190 0.87 +1.1 1,146,203 - RPSI07_RS13190 0.88 +0.3 1,146,287 + -1.2 1,146,287 + -0.0 1,146,287 + +0.1 1,146,288 - -1.5 1,146,288 - +0.1 1,146,288 - -0.5 1,146,366 - -1.4 1,146,366 - +2.4 1,146,366 - -1.2 1,146,504 + -0.1 1,146,515 - -1.1 1,146,583 + -1.2 1,146,583 + +0.0 1,146,583 + -0.2 1,146,827 + RPSI07_RS13195 0.38 -1.2 1,146,875 + RPSI07_RS13195 0.45 +0.4 1,146,875 + RPSI07_RS13195 0.45 -1.7 1,146,875 + RPSI07_RS13195 0.45 +0.1 1,146,876 - RPSI07_RS13195 0.45 -0.2 1,146,876 - RPSI07_RS13195 0.45 -0.3 1,146,882 - RPSI07_RS13195 0.46 +6.7 1,146,882 - RPSI07_RS13195 0.46 +1.1 1,146,882 - RPSI07_RS13195 0.46 +1.1
Or see this region's nucleotide sequence