Experiment: SMSA no antibiotic control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS02475 and RPSI07_RS02480 are separated by 179 nucleotides RPSI07_RS02480 and RPSI07_RS02485 are separated by 19 nucleotides RPSI07_RS02485 and RPSI07_RS02490 are separated by 9 nucleotides RPSI07_RS02490 and RPSI07_RS02495 are separated by 9 nucleotides
RPSI07_RS02475: RPSI07_RS02475 - MFS transporter, at 707,839 to 709,431
_RS02475
RPSI07_RS02480: RPSI07_RS02480 - transcriptional regulator, at 709,611 to 710,087
_RS02480
RPSI07_RS02485: RPSI07_RS02485 - magnesium and cobalt transport protein CorA, at 710,107 to 711,078
_RS02485
RPSI07_RS02490: RPSI07_RS02490 - DNA-3-methyladenine glycosylase I, at 711,088 to 711,660
_RS02490
RPSI07_RS02495: RPSI07_RS02495 - MFS transporter, at 711,670 to 712,893
_RS02495
Position (kb) 710
711
712 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 709.576 kb on - strand at 709.609 kb on + strand at 709.609 kb on + strand at 709.609 kb on + strand at 709.609 kb on + strand at 709.610 kb on - strand at 709.610 kb on - strand at 709.610 kb on - strand at 709.610 kb on - strand at 709.610 kb on - strand at 709.662 kb on + strand, within RPSI07_RS02480 at 709.662 kb on + strand, within RPSI07_RS02480 at 709.662 kb on + strand, within RPSI07_RS02480 at 709.662 kb on + strand, within RPSI07_RS02480 at 709.662 kb on + strand, within RPSI07_RS02480 at 709.662 kb on + strand, within RPSI07_RS02480 at 709.662 kb on + strand, within RPSI07_RS02480 at 709.662 kb on + strand, within RPSI07_RS02480 at 709.662 kb on + strand, within RPSI07_RS02480 at 709.663 kb on - strand, within RPSI07_RS02480 at 709.692 kb on + strand, within RPSI07_RS02480 at 709.692 kb on + strand, within RPSI07_RS02480 at 709.693 kb on - strand, within RPSI07_RS02480 at 709.693 kb on - strand, within RPSI07_RS02480 at 709.693 kb on - strand, within RPSI07_RS02480 at 710.332 kb on + strand, within RPSI07_RS02485 at 710.332 kb on + strand, within RPSI07_RS02485 at 710.332 kb on + strand, within RPSI07_RS02485 at 710.333 kb on - strand, within RPSI07_RS02485 at 710.333 kb on - strand, within RPSI07_RS02485 at 710.333 kb on - strand, within RPSI07_RS02485 at 710.461 kb on + strand, within RPSI07_RS02485 at 710.461 kb on + strand at 710.461 kb on + strand, within RPSI07_RS02485 at 710.461 kb on + strand, within RPSI07_RS02485 at 710.530 kb on + strand, within RPSI07_RS02485 at 710.560 kb on + strand, within RPSI07_RS02485 at 710.560 kb on + strand, within RPSI07_RS02485 at 710.560 kb on + strand, within RPSI07_RS02485 at 710.560 kb on + strand, within RPSI07_RS02485 at 710.561 kb on - strand, within RPSI07_RS02485 at 710.561 kb on - strand, within RPSI07_RS02485 at 710.656 kb on + strand, within RPSI07_RS02485 at 710.657 kb on - strand, within RPSI07_RS02485 at 710.657 kb on - strand, within RPSI07_RS02485 at 710.771 kb on - strand, within RPSI07_RS02485 at 710.771 kb on - strand, within RPSI07_RS02485 at 710.771 kb on - strand, within RPSI07_RS02485 at 710.953 kb on + strand, within RPSI07_RS02485 at 710.954 kb on - strand, within RPSI07_RS02485 at 710.954 kb on - strand, within RPSI07_RS02485 at 710.954 kb on - strand, within RPSI07_RS02485 at 710.954 kb on - strand, within RPSI07_RS02485 at 711.001 kb on + strand at 711.010 kb on + strand at 711.012 kb on - strand at 711.047 kb on - strand at 711.076 kb on + strand at 711.076 kb on + strand at 711.076 kb on + strand at 711.076 kb on + strand at 711.076 kb on + strand at 711.077 kb on - strand at 711.133 kb on + strand at 711.133 kb on + strand at 711.134 kb on - strand at 711.181 kb on + strand, within RPSI07_RS02490 at 711.181 kb on + strand, within RPSI07_RS02490 at 711.182 kb on - strand, within RPSI07_RS02490 at 711.182 kb on - strand, within RPSI07_RS02490 at 711.482 kb on - strand, within RPSI07_RS02490 at 711.482 kb on - strand, within RPSI07_RS02490 at 711.574 kb on + strand, within RPSI07_RS02490 at 711.574 kb on + strand, within RPSI07_RS02490 at 711.575 kb on - strand, within RPSI07_RS02490 at 711.803 kb on + strand, within RPSI07_RS02495 at 711.803 kb on + strand, within RPSI07_RS02495 at 711.803 kb on + strand, within RPSI07_RS02495 at 711.804 kb on - strand, within RPSI07_RS02495 at 711.828 kb on - strand, within RPSI07_RS02495 at 711.828 kb on - strand, within RPSI07_RS02495 at 711.977 kb on + strand, within RPSI07_RS02495 at 711.978 kb on - strand, within RPSI07_RS02495 at 711.978 kb on - strand, within RPSI07_RS02495 at 711.978 kb on - strand, within RPSI07_RS02495
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA no antibiotic control remove 709,576 - +0.6 709,609 + -0.5 709,609 + -1.8 709,609 + -0.5 709,609 + +0.3 709,610 - -0.0 709,610 - -0.9 709,610 - -2.0 709,610 - -0.4 709,610 - +1.8 709,662 + RPSI07_RS02480 0.11 -1.3 709,662 + RPSI07_RS02480 0.11 +0.5 709,662 + RPSI07_RS02480 0.11 +1.1 709,662 + RPSI07_RS02480 0.11 -2.1 709,662 + RPSI07_RS02480 0.11 -1.2 709,662 + RPSI07_RS02480 0.11 -2.3 709,662 + RPSI07_RS02480 0.11 -2.8 709,662 + RPSI07_RS02480 0.11 -2.1 709,662 + RPSI07_RS02480 0.11 -0.8 709,663 - RPSI07_RS02480 0.11 -1.2 709,692 + RPSI07_RS02480 0.17 +0.6 709,692 + RPSI07_RS02480 0.17 +1.9 709,693 - RPSI07_RS02480 0.17 -0.4 709,693 - RPSI07_RS02480 0.17 +0.2 709,693 - RPSI07_RS02480 0.17 -0.0 710,332 + RPSI07_RS02485 0.23 -2.1 710,332 + RPSI07_RS02485 0.23 +0.1 710,332 + RPSI07_RS02485 0.23 -0.5 710,333 - RPSI07_RS02485 0.23 +0.8 710,333 - RPSI07_RS02485 0.23 -0.1 710,333 - RPSI07_RS02485 0.23 +0.0 710,461 + RPSI07_RS02485 0.36 +0.2 710,461 + -2.0 710,461 + RPSI07_RS02485 0.36 +1.7 710,461 + RPSI07_RS02485 0.36 -0.8 710,530 + RPSI07_RS02485 0.44 -0.3 710,560 + RPSI07_RS02485 0.47 -0.9 710,560 + RPSI07_RS02485 0.47 +1.4 710,560 + RPSI07_RS02485 0.47 -0.7 710,560 + RPSI07_RS02485 0.47 -1.5 710,561 - RPSI07_RS02485 0.47 -0.2 710,561 - RPSI07_RS02485 0.47 +0.2 710,656 + RPSI07_RS02485 0.56 -0.9 710,657 - RPSI07_RS02485 0.57 -0.4 710,657 - RPSI07_RS02485 0.57 +0.1 710,771 - RPSI07_RS02485 0.68 +1.7 710,771 - RPSI07_RS02485 0.68 -1.8 710,771 - RPSI07_RS02485 0.68 -0.7 710,953 + RPSI07_RS02485 0.87 +0.2 710,954 - RPSI07_RS02485 0.87 +1.1 710,954 - RPSI07_RS02485 0.87 +0.3 710,954 - RPSI07_RS02485 0.87 -0.3 710,954 - RPSI07_RS02485 0.87 -0.5 711,001 + +0.8 711,010 + -1.3 711,012 - +0.6 711,047 - +1.5 711,076 + +0.0 711,076 + +0.7 711,076 + -0.1 711,076 + +0.5 711,076 + -0.3 711,077 - +2.9 711,133 + +0.5 711,133 + -1.6 711,134 - -0.0 711,181 + RPSI07_RS02490 0.16 -1.6 711,181 + RPSI07_RS02490 0.16 -0.8 711,182 - RPSI07_RS02490 0.16 +0.2 711,182 - RPSI07_RS02490 0.16 -0.2 711,482 - RPSI07_RS02490 0.69 +0.4 711,482 - RPSI07_RS02490 0.69 +0.2 711,574 + RPSI07_RS02490 0.85 +0.8 711,574 + RPSI07_RS02490 0.85 +0.3 711,575 - RPSI07_RS02490 0.85 +0.6 711,803 + RPSI07_RS02495 0.11 -0.8 711,803 + RPSI07_RS02495 0.11 -0.0 711,803 + RPSI07_RS02495 0.11 -1.1 711,804 - RPSI07_RS02495 0.11 -0.8 711,828 - RPSI07_RS02495 0.13 +0.2 711,828 - RPSI07_RS02495 0.13 +1.2 711,977 + RPSI07_RS02495 0.25 +0.4 711,978 - RPSI07_RS02495 0.25 -0.0 711,978 - RPSI07_RS02495 0.25 -0.9 711,978 - RPSI07_RS02495 0.25 -0.9
Or see this region's nucleotide sequence