Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol_and_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS22760 and RPSI07_RS22765 overlap by 1 nucleotides RPSI07_RS22765 and RPSI07_RS22770 are separated by 97 nucleotides RPSI07_RS22770 and RPSI07_RS22775 are separated by 19 nucleotides
RPSI07_RS22760: RPSI07_RS22760 - short-chain dehydrogenase, at 3,255,773 to 3,256,573
_RS22760
RPSI07_RS22765: RPSI07_RS22765 - periplasmic metal binding protein, at 3,256,573 to 3,257,418
_RS22765
RPSI07_RS22770: RPSI07_RS22770 - antibiotic biosynthesis monooxygenase, at 3,257,516 to 3,257,902
_RS22770
RPSI07_RS22775: RPSI07_RS22775 - Zn-dependent hydrolase, at 3,257,922 to 3,259,001
_RS22775
Position (kb)
3257
3258 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3256.517 kb on + strand at 3256.518 kb on - strand at 3256.918 kb on + strand, within RPSI07_RS22765 at 3256.919 kb on - strand, within RPSI07_RS22765 at 3257.036 kb on - strand, within RPSI07_RS22765 at 3257.036 kb on - strand, within RPSI07_RS22765 at 3257.080 kb on + strand, within RPSI07_RS22765 at 3257.080 kb on + strand, within RPSI07_RS22765 at 3257.080 kb on + strand, within RPSI07_RS22765 at 3257.080 kb on + strand, within RPSI07_RS22765 at 3257.081 kb on - strand, within RPSI07_RS22765 at 3257.081 kb on - strand, within RPSI07_RS22765 at 3257.081 kb on - strand, within RPSI07_RS22765 at 3257.081 kb on - strand, within RPSI07_RS22765 at 3257.270 kb on - strand, within RPSI07_RS22765 at 3257.270 kb on - strand, within RPSI07_RS22765 at 3257.327 kb on - strand, within RPSI07_RS22765 at 3257.386 kb on + strand at 3257.538 kb on + strand at 3257.538 kb on + strand at 3257.566 kb on - strand, within RPSI07_RS22770 at 3257.575 kb on - strand, within RPSI07_RS22770 at 3257.658 kb on + strand, within RPSI07_RS22770 at 3257.658 kb on + strand, within RPSI07_RS22770 at 3257.659 kb on - strand, within RPSI07_RS22770 at 3257.826 kb on + strand, within RPSI07_RS22770 at 3257.826 kb on + strand, within RPSI07_RS22770 at 3257.827 kb on - strand, within RPSI07_RS22770 at 3258.232 kb on + strand, within RPSI07_RS22775 at 3258.232 kb on + strand, within RPSI07_RS22775 at 3258.233 kb on - strand, within RPSI07_RS22775 at 3258.233 kb on - strand, within RPSI07_RS22775 at 3258.233 kb on - strand, within RPSI07_RS22775 at 3258.233 kb on - strand, within RPSI07_RS22775 at 3258.233 kb on - strand, within RPSI07_RS22775 at 3258.362 kb on - strand, within RPSI07_RS22775 at 3258.457 kb on + strand, within RPSI07_RS22775 at 3258.490 kb on + strand, within RPSI07_RS22775 at 3258.490 kb on + strand, within RPSI07_RS22775 at 3258.490 kb on + strand, within RPSI07_RS22775 at 3258.491 kb on - strand, within RPSI07_RS22775 at 3258.491 kb on - strand, within RPSI07_RS22775 at 3258.491 kb on - strand, within RPSI07_RS22775 at 3258.493 kb on + strand, within RPSI07_RS22775 at 3258.494 kb on - strand, within RPSI07_RS22775 at 3258.625 kb on + strand, within RPSI07_RS22775 at 3258.626 kb on - strand, within RPSI07_RS22775 at 3258.733 kb on - strand, within RPSI07_RS22775 at 3258.733 kb on - strand, within RPSI07_RS22775 at 3258.733 kb on - strand, within RPSI07_RS22775
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol_and_CrystalViolet remove 3,256,517 + +0.8 3,256,518 - -0.0 3,256,918 + RPSI07_RS22765 0.41 +0.0 3,256,919 - RPSI07_RS22765 0.41 -0.3 3,257,036 - RPSI07_RS22765 0.55 +0.9 3,257,036 - RPSI07_RS22765 0.55 +1.0 3,257,080 + RPSI07_RS22765 0.60 +0.5 3,257,080 + RPSI07_RS22765 0.60 -0.4 3,257,080 + RPSI07_RS22765 0.60 +1.0 3,257,080 + RPSI07_RS22765 0.60 -0.4 3,257,081 - RPSI07_RS22765 0.60 +0.2 3,257,081 - RPSI07_RS22765 0.60 +0.5 3,257,081 - RPSI07_RS22765 0.60 +0.7 3,257,081 - RPSI07_RS22765 0.60 +1.4 3,257,270 - RPSI07_RS22765 0.82 -0.4 3,257,270 - RPSI07_RS22765 0.82 -1.1 3,257,327 - RPSI07_RS22765 0.89 +0.6 3,257,386 + -3.2 3,257,538 + +0.4 3,257,538 + -0.9 3,257,566 - RPSI07_RS22770 0.13 +0.2 3,257,575 - RPSI07_RS22770 0.15 -1.4 3,257,658 + RPSI07_RS22770 0.37 -0.6 3,257,658 + RPSI07_RS22770 0.37 -0.3 3,257,659 - RPSI07_RS22770 0.37 -1.0 3,257,826 + RPSI07_RS22770 0.80 +0.5 3,257,826 + RPSI07_RS22770 0.80 +1.4 3,257,827 - RPSI07_RS22770 0.80 +3.0 3,258,232 + RPSI07_RS22775 0.29 -0.0 3,258,232 + RPSI07_RS22775 0.29 +0.7 3,258,233 - RPSI07_RS22775 0.29 +0.2 3,258,233 - RPSI07_RS22775 0.29 -1.0 3,258,233 - RPSI07_RS22775 0.29 +0.4 3,258,233 - RPSI07_RS22775 0.29 +0.7 3,258,233 - RPSI07_RS22775 0.29 -0.8 3,258,362 - RPSI07_RS22775 0.41 +1.4 3,258,457 + RPSI07_RS22775 0.50 -1.7 3,258,490 + RPSI07_RS22775 0.53 -1.5 3,258,490 + RPSI07_RS22775 0.53 -0.8 3,258,490 + RPSI07_RS22775 0.53 +1.1 3,258,491 - RPSI07_RS22775 0.53 +0.1 3,258,491 - RPSI07_RS22775 0.53 -1.1 3,258,491 - RPSI07_RS22775 0.53 +1.4 3,258,493 + RPSI07_RS22775 0.53 +0.8 3,258,494 - RPSI07_RS22775 0.53 -0.9 3,258,625 + RPSI07_RS22775 0.65 -0.2 3,258,626 - RPSI07_RS22775 0.65 +0.2 3,258,733 - RPSI07_RS22775 0.75 +1.2 3,258,733 - RPSI07_RS22775 0.75 -1.4 3,258,733 - RPSI07_RS22775 0.75 +1.4
Or see this region's nucleotide sequence