Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol_and_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS19310 and RPSI07_RS19315 are separated by 78 nucleotides RPSI07_RS19315 and RPSI07_RS19320 are separated by 8 nucleotides RPSI07_RS19320 and RPSI07_RS19325 are separated by 181 nucleotides RPSI07_RS19325 and RPSI07_RS19330 overlap by 4 nucleotides
RPSI07_RS19310: RPSI07_RS19310 - VWA domain-containing protein, at 2,515,989 to 2,517,164
_RS19310
RPSI07_RS19315: RPSI07_RS19315 - spermidine synthase, at 2,517,243 to 2,518,118
_RS19315
RPSI07_RS19320: RPSI07_RS19320 - membrane protein, at 2,518,127 to 2,518,885
_RS19320
RPSI07_RS19325: RPSI07_RS19325 - transcriptional regulator, at 2,519,067 to 2,519,450
_RS19325
RPSI07_RS19330: RPSI07_RS19330 - glutathione S-transferase, at 2,519,447 to 2,520,529
_RS19330
Position (kb)
2518
2519 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 2517.162 kb on + strand at 2517.162 kb on + strand at 2517.162 kb on + strand at 2517.163 kb on - strand at 2517.163 kb on - strand at 2517.509 kb on + strand, within RPSI07_RS19315 at 2517.510 kb on - strand, within RPSI07_RS19315 at 2517.553 kb on + strand, within RPSI07_RS19315 at 2517.554 kb on - strand, within RPSI07_RS19315 at 2517.598 kb on + strand, within RPSI07_RS19315 at 2517.599 kb on - strand, within RPSI07_RS19315 at 2517.658 kb on + strand, within RPSI07_RS19315 at 2517.658 kb on + strand, within RPSI07_RS19315 at 2517.965 kb on - strand, within RPSI07_RS19315 at 2517.965 kb on - strand, within RPSI07_RS19315 at 2518.178 kb on - strand at 2518.191 kb on + strand at 2518.290 kb on + strand, within RPSI07_RS19320 at 2518.290 kb on + strand, within RPSI07_RS19320 at 2518.291 kb on - strand, within RPSI07_RS19320 at 2518.386 kb on + strand, within RPSI07_RS19320 at 2518.386 kb on + strand, within RPSI07_RS19320 at 2518.387 kb on - strand, within RPSI07_RS19320 at 2518.722 kb on + strand, within RPSI07_RS19320 at 2518.740 kb on + strand, within RPSI07_RS19320 at 2518.741 kb on - strand, within RPSI07_RS19320 at 2518.755 kb on + strand, within RPSI07_RS19320 at 2518.755 kb on + strand, within RPSI07_RS19320 at 2518.756 kb on - strand, within RPSI07_RS19320 at 2518.756 kb on - strand, within RPSI07_RS19320 at 2518.801 kb on + strand, within RPSI07_RS19320 at 2518.803 kb on + strand, within RPSI07_RS19320 at 2518.803 kb on + strand, within RPSI07_RS19320 at 2518.803 kb on + strand, within RPSI07_RS19320 at 2518.803 kb on + strand, within RPSI07_RS19320 at 2518.804 kb on - strand, within RPSI07_RS19320 at 2518.804 kb on - strand, within RPSI07_RS19320 at 2518.804 kb on - strand, within RPSI07_RS19320 at 2518.951 kb on + strand at 2518.951 kb on + strand at 2518.952 kb on - strand at 2518.952 kb on - strand at 2519.037 kb on - strand at 2519.037 kb on - strand at 2519.057 kb on - strand at 2519.699 kb on + strand, within RPSI07_RS19330 at 2519.700 kb on - strand, within RPSI07_RS19330 at 2519.879 kb on + strand, within RPSI07_RS19330
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol_and_CrystalViolet remove 2,517,162 + +0.6 2,517,162 + -1.1 2,517,162 + +1.4 2,517,163 - -0.4 2,517,163 - -0.8 2,517,509 + RPSI07_RS19315 0.30 +0.3 2,517,510 - RPSI07_RS19315 0.30 +1.4 2,517,553 + RPSI07_RS19315 0.35 +1.1 2,517,554 - RPSI07_RS19315 0.36 -1.7 2,517,598 + RPSI07_RS19315 0.41 -0.1 2,517,599 - RPSI07_RS19315 0.41 -0.4 2,517,658 + RPSI07_RS19315 0.47 -0.1 2,517,658 + RPSI07_RS19315 0.47 +0.5 2,517,965 - RPSI07_RS19315 0.82 +3.8 2,517,965 - RPSI07_RS19315 0.82 -1.6 2,518,178 - -1.3 2,518,191 + -0.5 2,518,290 + RPSI07_RS19320 0.21 -0.3 2,518,290 + RPSI07_RS19320 0.21 -1.7 2,518,291 - RPSI07_RS19320 0.22 -0.8 2,518,386 + RPSI07_RS19320 0.34 -1.7 2,518,386 + RPSI07_RS19320 0.34 -1.3 2,518,387 - RPSI07_RS19320 0.34 -0.9 2,518,722 + RPSI07_RS19320 0.78 -2.2 2,518,740 + RPSI07_RS19320 0.81 +0.0 2,518,741 - RPSI07_RS19320 0.81 -0.1 2,518,755 + RPSI07_RS19320 0.83 -2.4 2,518,755 + RPSI07_RS19320 0.83 -0.7 2,518,756 - RPSI07_RS19320 0.83 +1.3 2,518,756 - RPSI07_RS19320 0.83 +0.5 2,518,801 + RPSI07_RS19320 0.89 +0.3 2,518,803 + RPSI07_RS19320 0.89 +0.4 2,518,803 + RPSI07_RS19320 0.89 -1.1 2,518,803 + RPSI07_RS19320 0.89 -2.2 2,518,803 + RPSI07_RS19320 0.89 -0.5 2,518,804 - RPSI07_RS19320 0.89 -2.3 2,518,804 - RPSI07_RS19320 0.89 +0.2 2,518,804 - RPSI07_RS19320 0.89 +1.3 2,518,951 + -0.3 2,518,951 + +0.2 2,518,952 - -1.0 2,518,952 - -0.7 2,519,037 - +1.3 2,519,037 - +0.9 2,519,057 - -0.3 2,519,699 + RPSI07_RS19330 0.23 -2.5 2,519,700 - RPSI07_RS19330 0.23 +0.4 2,519,879 + RPSI07_RS19330 0.40 +0.3
Or see this region's nucleotide sequence