Strain Fitness in Ralstonia solanacearum PSI07 around RPSI07_RS19320

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol_and_CrystalViolet

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRPSI07_RS19310 and RPSI07_RS19315 are separated by 78 nucleotidesRPSI07_RS19315 and RPSI07_RS19320 are separated by 8 nucleotidesRPSI07_RS19320 and RPSI07_RS19325 are separated by 181 nucleotidesRPSI07_RS19325 and RPSI07_RS19330 overlap by 4 nucleotides RPSI07_RS19310: RPSI07_RS19310 - VWA domain-containing protein, at 2,515,989 to 2,517,164 _RS19310 RPSI07_RS19315: RPSI07_RS19315 - spermidine synthase, at 2,517,243 to 2,518,118 _RS19315 RPSI07_RS19320: RPSI07_RS19320 - membrane protein, at 2,518,127 to 2,518,885 _RS19320 RPSI07_RS19325: RPSI07_RS19325 - transcriptional regulator, at 2,519,067 to 2,519,450 _RS19325 RPSI07_RS19330: RPSI07_RS19330 - glutathione S-transferase, at 2,519,447 to 2,520,529 _RS19330 Position (kb) 2518 2519Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 2517.162 kb on + strandat 2517.162 kb on + strandat 2517.162 kb on + strandat 2517.163 kb on - strandat 2517.163 kb on - strandat 2517.509 kb on + strand, within RPSI07_RS19315at 2517.510 kb on - strand, within RPSI07_RS19315at 2517.553 kb on + strand, within RPSI07_RS19315at 2517.554 kb on - strand, within RPSI07_RS19315at 2517.598 kb on + strand, within RPSI07_RS19315at 2517.599 kb on - strand, within RPSI07_RS19315at 2517.658 kb on + strand, within RPSI07_RS19315at 2517.658 kb on + strand, within RPSI07_RS19315at 2517.965 kb on - strand, within RPSI07_RS19315at 2517.965 kb on - strand, within RPSI07_RS19315at 2518.178 kb on - strandat 2518.191 kb on + strandat 2518.290 kb on + strand, within RPSI07_RS19320at 2518.290 kb on + strand, within RPSI07_RS19320at 2518.291 kb on - strand, within RPSI07_RS19320at 2518.386 kb on + strand, within RPSI07_RS19320at 2518.386 kb on + strand, within RPSI07_RS19320at 2518.387 kb on - strand, within RPSI07_RS19320at 2518.722 kb on + strand, within RPSI07_RS19320at 2518.740 kb on + strand, within RPSI07_RS19320at 2518.741 kb on - strand, within RPSI07_RS19320at 2518.755 kb on + strand, within RPSI07_RS19320at 2518.755 kb on + strand, within RPSI07_RS19320at 2518.756 kb on - strand, within RPSI07_RS19320at 2518.756 kb on - strand, within RPSI07_RS19320at 2518.801 kb on + strand, within RPSI07_RS19320at 2518.803 kb on + strand, within RPSI07_RS19320at 2518.803 kb on + strand, within RPSI07_RS19320at 2518.803 kb on + strand, within RPSI07_RS19320at 2518.803 kb on + strand, within RPSI07_RS19320at 2518.804 kb on - strand, within RPSI07_RS19320at 2518.804 kb on - strand, within RPSI07_RS19320at 2518.804 kb on - strand, within RPSI07_RS19320at 2518.951 kb on + strandat 2518.951 kb on + strandat 2518.952 kb on - strandat 2518.952 kb on - strandat 2519.037 kb on - strandat 2519.037 kb on - strandat 2519.057 kb on - strandat 2519.699 kb on + strand, within RPSI07_RS19330at 2519.700 kb on - strand, within RPSI07_RS19330at 2519.879 kb on + strand, within RPSI07_RS19330

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol_and_CrystalViolet
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2,517,162 + +0.6
2,517,162 + -1.1
2,517,162 + +1.4
2,517,163 - -0.4
2,517,163 - -0.8
2,517,509 + RPSI07_RS19315 0.30 +0.3
2,517,510 - RPSI07_RS19315 0.30 +1.4
2,517,553 + RPSI07_RS19315 0.35 +1.1
2,517,554 - RPSI07_RS19315 0.36 -1.7
2,517,598 + RPSI07_RS19315 0.41 -0.1
2,517,599 - RPSI07_RS19315 0.41 -0.4
2,517,658 + RPSI07_RS19315 0.47 -0.1
2,517,658 + RPSI07_RS19315 0.47 +0.5
2,517,965 - RPSI07_RS19315 0.82 +3.8
2,517,965 - RPSI07_RS19315 0.82 -1.6
2,518,178 - -1.3
2,518,191 + -0.5
2,518,290 + RPSI07_RS19320 0.21 -0.3
2,518,290 + RPSI07_RS19320 0.21 -1.7
2,518,291 - RPSI07_RS19320 0.22 -0.8
2,518,386 + RPSI07_RS19320 0.34 -1.7
2,518,386 + RPSI07_RS19320 0.34 -1.3
2,518,387 - RPSI07_RS19320 0.34 -0.9
2,518,722 + RPSI07_RS19320 0.78 -2.2
2,518,740 + RPSI07_RS19320 0.81 +0.0
2,518,741 - RPSI07_RS19320 0.81 -0.1
2,518,755 + RPSI07_RS19320 0.83 -2.4
2,518,755 + RPSI07_RS19320 0.83 -0.7
2,518,756 - RPSI07_RS19320 0.83 +1.3
2,518,756 - RPSI07_RS19320 0.83 +0.5
2,518,801 + RPSI07_RS19320 0.89 +0.3
2,518,803 + RPSI07_RS19320 0.89 +0.4
2,518,803 + RPSI07_RS19320 0.89 -1.1
2,518,803 + RPSI07_RS19320 0.89 -2.2
2,518,803 + RPSI07_RS19320 0.89 -0.5
2,518,804 - RPSI07_RS19320 0.89 -2.3
2,518,804 - RPSI07_RS19320 0.89 +0.2
2,518,804 - RPSI07_RS19320 0.89 +1.3
2,518,951 + -0.3
2,518,951 + +0.2
2,518,952 - -1.0
2,518,952 - -0.7
2,519,037 - +1.3
2,519,037 - +0.9
2,519,057 - -0.3
2,519,699 + RPSI07_RS19330 0.23 -2.5
2,519,700 - RPSI07_RS19330 0.23 +0.4
2,519,879 + RPSI07_RS19330 0.40 +0.3

Or see this region's nucleotide sequence