Strain Fitness in Ralstonia solanacearum PSI07 around RPSI07_RS17865

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol_and_CrystalViolet

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRPSI07_RS17860 and RPSI07_RS17865 are separated by 133 nucleotidesRPSI07_RS17865 and RPSI07_RS17870 are separated by 83 nucleotides RPSI07_RS17860: RPSI07_RS17860 - YncE family protein, at 2,210,382 to 2,211,374 _RS17860 RPSI07_RS17865: RPSI07_RS17865 - ATP-dependent RNA helicase HrpA, at 2,211,508 to 2,215,488 _RS17865 RPSI07_RS17870: RPSI07_RS17870 - amino-acid N-acetyltransferase, at 2,215,572 to 2,216,969 _RS17870 Position (kb) 2211 2212 2213 2214 2215 2216Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 2210.986 kb on + strandat 2210.987 kb on - strand, within RPSI07_RS17860at 2211.374 kb on + strandat 2211.374 kb on + strandat 2211.374 kb on + strandat 2211.374 kb on + strandat 2211.374 kb on + strandat 2211.375 kb on - strandat 2211.375 kb on - strandat 2211.395 kb on + strandat 2211.648 kb on + strandat 2211.648 kb on + strandat 2211.933 kb on - strand, within RPSI07_RS17865at 2212.121 kb on + strand, within RPSI07_RS17865at 2212.122 kb on - strand, within RPSI07_RS17865at 2212.122 kb on - strand, within RPSI07_RS17865at 2212.166 kb on + strand, within RPSI07_RS17865at 2212.167 kb on - strand, within RPSI07_RS17865at 2212.190 kb on - strand, within RPSI07_RS17865at 2212.190 kb on - strand, within RPSI07_RS17865at 2212.377 kb on - strand, within RPSI07_RS17865at 2212.841 kb on + strand, within RPSI07_RS17865at 2212.842 kb on - strand, within RPSI07_RS17865at 2212.895 kb on + strand, within RPSI07_RS17865at 2212.896 kb on - strand, within RPSI07_RS17865at 2212.997 kb on + strand, within RPSI07_RS17865at 2212.997 kb on + strand, within RPSI07_RS17865at 2213.033 kb on + strand, within RPSI07_RS17865at 2213.087 kb on + strand, within RPSI07_RS17865at 2213.088 kb on - strand, within RPSI07_RS17865at 2213.088 kb on - strand, within RPSI07_RS17865at 2213.088 kb on - strand, within RPSI07_RS17865at 2213.295 kb on - strand, within RPSI07_RS17865at 2213.295 kb on - strand, within RPSI07_RS17865at 2214.086 kb on + strand, within RPSI07_RS17865at 2214.249 kb on - strand, within RPSI07_RS17865at 2214.362 kb on + strand, within RPSI07_RS17865at 2214.398 kb on + strand, within RPSI07_RS17865at 2214.861 kb on - strand, within RPSI07_RS17865at 2215.113 kb on + strandat 2215.114 kb on - strandat 2215.499 kb on + strandat 2215.501 kb on + strandat 2215.501 kb on + strandat 2215.501 kb on + strandat 2215.708 kb on - strandat 2215.708 kb on - strandat 2215.708 kb on - strandat 2216.388 kb on + strand, within RPSI07_RS17870

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol_and_CrystalViolet
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2,210,986 + +2.9
2,210,987 - RPSI07_RS17860 0.61 -1.7
2,211,374 + -3.0
2,211,374 + -1.5
2,211,374 + -1.2
2,211,374 + -3.2
2,211,374 + -1.1
2,211,375 - -0.6
2,211,375 - -1.5
2,211,395 + +0.7
2,211,648 + -0.4
2,211,648 + -1.6
2,211,933 - RPSI07_RS17865 0.11 +0.1
2,212,121 + RPSI07_RS17865 0.15 -0.6
2,212,122 - RPSI07_RS17865 0.15 -0.2
2,212,122 - RPSI07_RS17865 0.15 -3.3
2,212,166 + RPSI07_RS17865 0.17 -0.2
2,212,167 - RPSI07_RS17865 0.17 -1.3
2,212,190 - RPSI07_RS17865 0.17 +0.8
2,212,190 - RPSI07_RS17865 0.17 -0.8
2,212,377 - RPSI07_RS17865 0.22 +1.1
2,212,841 + RPSI07_RS17865 0.33 -0.4
2,212,842 - RPSI07_RS17865 0.34 -1.5
2,212,895 + RPSI07_RS17865 0.35 -1.0
2,212,896 - RPSI07_RS17865 0.35 -0.8
2,212,997 + RPSI07_RS17865 0.37 +0.7
2,212,997 + RPSI07_RS17865 0.37 -1.0
2,213,033 + RPSI07_RS17865 0.38 -1.0
2,213,087 + RPSI07_RS17865 0.40 +0.2
2,213,088 - RPSI07_RS17865 0.40 -2.6
2,213,088 - RPSI07_RS17865 0.40 +0.5
2,213,088 - RPSI07_RS17865 0.40 -0.5
2,213,295 - RPSI07_RS17865 0.45 +0.6
2,213,295 - RPSI07_RS17865 0.45 +1.3
2,214,086 + RPSI07_RS17865 0.65 +2.3
2,214,249 - RPSI07_RS17865 0.69 +0.1
2,214,362 + RPSI07_RS17865 0.72 +3.2
2,214,398 + RPSI07_RS17865 0.73 +2.1
2,214,861 - RPSI07_RS17865 0.84 -1.4
2,215,113 + +0.1
2,215,114 - +0.8
2,215,499 + -0.5
2,215,501 + +0.6
2,215,501 + -0.7
2,215,501 + +1.0
2,215,708 - -0.7
2,215,708 - -0.7
2,215,708 - -0.9
2,216,388 + RPSI07_RS17870 0.58 +1.1

Or see this region's nucleotide sequence