Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol_and_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS17860 and RPSI07_RS17865 are separated by 133 nucleotides RPSI07_RS17865 and RPSI07_RS17870 are separated by 83 nucleotides
RPSI07_RS17860: RPSI07_RS17860 - YncE family protein, at 2,210,382 to 2,211,374
_RS17860
RPSI07_RS17865: RPSI07_RS17865 - ATP-dependent RNA helicase HrpA, at 2,211,508 to 2,215,488
_RS17865
RPSI07_RS17870: RPSI07_RS17870 - amino-acid N-acetyltransferase, at 2,215,572 to 2,216,969
_RS17870
Position (kb)
2211
2212
2213
2214
2215
2216 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2210.986 kb on + strand at 2210.987 kb on - strand, within RPSI07_RS17860 at 2211.374 kb on + strand at 2211.374 kb on + strand at 2211.374 kb on + strand at 2211.374 kb on + strand at 2211.374 kb on + strand at 2211.375 kb on - strand at 2211.375 kb on - strand at 2211.395 kb on + strand at 2211.648 kb on + strand at 2211.648 kb on + strand at 2211.933 kb on - strand, within RPSI07_RS17865 at 2212.121 kb on + strand, within RPSI07_RS17865 at 2212.122 kb on - strand, within RPSI07_RS17865 at 2212.122 kb on - strand, within RPSI07_RS17865 at 2212.166 kb on + strand, within RPSI07_RS17865 at 2212.167 kb on - strand, within RPSI07_RS17865 at 2212.190 kb on - strand, within RPSI07_RS17865 at 2212.190 kb on - strand, within RPSI07_RS17865 at 2212.377 kb on - strand, within RPSI07_RS17865 at 2212.841 kb on + strand, within RPSI07_RS17865 at 2212.842 kb on - strand, within RPSI07_RS17865 at 2212.895 kb on + strand, within RPSI07_RS17865 at 2212.896 kb on - strand, within RPSI07_RS17865 at 2212.997 kb on + strand, within RPSI07_RS17865 at 2212.997 kb on + strand, within RPSI07_RS17865 at 2213.033 kb on + strand, within RPSI07_RS17865 at 2213.087 kb on + strand, within RPSI07_RS17865 at 2213.088 kb on - strand, within RPSI07_RS17865 at 2213.088 kb on - strand, within RPSI07_RS17865 at 2213.088 kb on - strand, within RPSI07_RS17865 at 2213.295 kb on - strand, within RPSI07_RS17865 at 2213.295 kb on - strand, within RPSI07_RS17865 at 2214.086 kb on + strand, within RPSI07_RS17865 at 2214.249 kb on - strand, within RPSI07_RS17865 at 2214.362 kb on + strand, within RPSI07_RS17865 at 2214.398 kb on + strand, within RPSI07_RS17865 at 2214.861 kb on - strand, within RPSI07_RS17865 at 2215.113 kb on + strand at 2215.114 kb on - strand at 2215.499 kb on + strand at 2215.501 kb on + strand at 2215.501 kb on + strand at 2215.501 kb on + strand at 2215.708 kb on - strand at 2215.708 kb on - strand at 2215.708 kb on - strand at 2216.388 kb on + strand, within RPSI07_RS17870
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol_and_CrystalViolet remove 2,210,986 + +2.9 2,210,987 - RPSI07_RS17860 0.61 -1.7 2,211,374 + -3.0 2,211,374 + -1.5 2,211,374 + -1.2 2,211,374 + -3.2 2,211,374 + -1.1 2,211,375 - -0.6 2,211,375 - -1.5 2,211,395 + +0.7 2,211,648 + -0.4 2,211,648 + -1.6 2,211,933 - RPSI07_RS17865 0.11 +0.1 2,212,121 + RPSI07_RS17865 0.15 -0.6 2,212,122 - RPSI07_RS17865 0.15 -0.2 2,212,122 - RPSI07_RS17865 0.15 -3.3 2,212,166 + RPSI07_RS17865 0.17 -0.2 2,212,167 - RPSI07_RS17865 0.17 -1.3 2,212,190 - RPSI07_RS17865 0.17 +0.8 2,212,190 - RPSI07_RS17865 0.17 -0.8 2,212,377 - RPSI07_RS17865 0.22 +1.1 2,212,841 + RPSI07_RS17865 0.33 -0.4 2,212,842 - RPSI07_RS17865 0.34 -1.5 2,212,895 + RPSI07_RS17865 0.35 -1.0 2,212,896 - RPSI07_RS17865 0.35 -0.8 2,212,997 + RPSI07_RS17865 0.37 +0.7 2,212,997 + RPSI07_RS17865 0.37 -1.0 2,213,033 + RPSI07_RS17865 0.38 -1.0 2,213,087 + RPSI07_RS17865 0.40 +0.2 2,213,088 - RPSI07_RS17865 0.40 -2.6 2,213,088 - RPSI07_RS17865 0.40 +0.5 2,213,088 - RPSI07_RS17865 0.40 -0.5 2,213,295 - RPSI07_RS17865 0.45 +0.6 2,213,295 - RPSI07_RS17865 0.45 +1.3 2,214,086 + RPSI07_RS17865 0.65 +2.3 2,214,249 - RPSI07_RS17865 0.69 +0.1 2,214,362 + RPSI07_RS17865 0.72 +3.2 2,214,398 + RPSI07_RS17865 0.73 +2.1 2,214,861 - RPSI07_RS17865 0.84 -1.4 2,215,113 + +0.1 2,215,114 - +0.8 2,215,499 + -0.5 2,215,501 + +0.6 2,215,501 + -0.7 2,215,501 + +1.0 2,215,708 - -0.7 2,215,708 - -0.7 2,215,708 - -0.9 2,216,388 + RPSI07_RS17870 0.58 +1.1
Or see this region's nucleotide sequence