Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol_and_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS09880 and RPSI07_RS09885 are separated by 68 nucleotides RPSI07_RS09885 and RPSI07_RS09890 are separated by 140 nucleotides
RPSI07_RS09880: RPSI07_RS09880 - ABC transporter ATP-binding protein, at 435,377 to 436,498
_RS09880
RPSI07_RS09885: RPSI07_RS09885 - glycerol-3-phosphate dehydrogenase, at 436,567 to 438,144
_RS09885
RPSI07_RS09890: RPSI07_RS09890 - DeoR/GlpR transcriptional regulator, at 438,285 to 439,049
_RS09890
Position (kb) 436
437
438
439 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5
6 at 435.570 kb on + strand, within RPSI07_RS09880 at 435.570 kb on + strand, within RPSI07_RS09880 at 435.570 kb on + strand, within RPSI07_RS09880 at 435.570 kb on + strand, within RPSI07_RS09880 at 435.570 kb on + strand, within RPSI07_RS09880 at 435.571 kb on - strand, within RPSI07_RS09880 at 435.571 kb on - strand, within RPSI07_RS09880 at 435.571 kb on - strand, within RPSI07_RS09880 at 435.834 kb on + strand, within RPSI07_RS09880 at 435.834 kb on + strand, within RPSI07_RS09880 at 435.834 kb on + strand, within RPSI07_RS09880 at 435.834 kb on + strand, within RPSI07_RS09880 at 435.835 kb on - strand, within RPSI07_RS09880 at 435.835 kb on - strand, within RPSI07_RS09880 at 435.835 kb on - strand, within RPSI07_RS09880 at 435.835 kb on - strand, within RPSI07_RS09880 at 435.835 kb on - strand, within RPSI07_RS09880 at 435.835 kb on - strand, within RPSI07_RS09880 at 435.933 kb on + strand, within RPSI07_RS09880 at 435.933 kb on + strand, within RPSI07_RS09880 at 435.933 kb on + strand, within RPSI07_RS09880 at 435.933 kb on + strand, within RPSI07_RS09880 at 435.933 kb on + strand, within RPSI07_RS09880 at 435.934 kb on - strand, within RPSI07_RS09880 at 435.934 kb on - strand, within RPSI07_RS09880 at 435.934 kb on - strand, within RPSI07_RS09880 at 435.934 kb on - strand, within RPSI07_RS09880 at 435.934 kb on - strand, within RPSI07_RS09880 at 436.003 kb on - strand, within RPSI07_RS09880 at 436.003 kb on - strand, within RPSI07_RS09880 at 436.003 kb on - strand, within RPSI07_RS09880 at 436.003 kb on - strand, within RPSI07_RS09880 at 436.224 kb on + strand, within RPSI07_RS09880 at 436.224 kb on + strand, within RPSI07_RS09880 at 436.224 kb on + strand, within RPSI07_RS09880 at 436.224 kb on + strand, within RPSI07_RS09880 at 436.224 kb on + strand, within RPSI07_RS09880 at 436.225 kb on - strand, within RPSI07_RS09880 at 436.225 kb on - strand, within RPSI07_RS09880 at 436.225 kb on - strand, within RPSI07_RS09880 at 436.225 kb on - strand, within RPSI07_RS09880 at 436.225 kb on - strand, within RPSI07_RS09880 at 436.242 kb on + strand, within RPSI07_RS09880 at 436.243 kb on - strand, within RPSI07_RS09880 at 436.260 kb on + strand, within RPSI07_RS09880 at 436.260 kb on + strand, within RPSI07_RS09880 at 436.260 kb on + strand, within RPSI07_RS09880 at 436.261 kb on - strand, within RPSI07_RS09880 at 436.261 kb on - strand, within RPSI07_RS09880 at 436.323 kb on - strand, within RPSI07_RS09880 at 436.563 kb on + strand at 436.563 kb on + strand at 436.564 kb on - strand at 436.564 kb on - strand at 436.564 kb on - strand at 436.564 kb on - strand at 436.564 kb on - strand at 436.564 kb on - strand at 436.564 kb on - strand at 436.564 kb on - strand at 436.564 kb on - strand at 436.748 kb on + strand, within RPSI07_RS09885 at 436.748 kb on + strand, within RPSI07_RS09885 at 436.749 kb on - strand, within RPSI07_RS09885 at 436.838 kb on + strand, within RPSI07_RS09885 at 436.838 kb on + strand, within RPSI07_RS09885 at 436.910 kb on + strand, within RPSI07_RS09885 at 436.910 kb on + strand, within RPSI07_RS09885 at 437.042 kb on + strand, within RPSI07_RS09885 at 437.042 kb on + strand, within RPSI07_RS09885 at 437.043 kb on - strand, within RPSI07_RS09885 at 437.120 kb on + strand, within RPSI07_RS09885 at 437.120 kb on + strand, within RPSI07_RS09885 at 437.120 kb on + strand, within RPSI07_RS09885 at 437.120 kb on + strand, within RPSI07_RS09885 at 437.121 kb on - strand, within RPSI07_RS09885 at 437.121 kb on - strand, within RPSI07_RS09885 at 437.184 kb on - strand, within RPSI07_RS09885 at 437.184 kb on - strand, within RPSI07_RS09885 at 437.297 kb on + strand, within RPSI07_RS09885 at 437.298 kb on - strand, within RPSI07_RS09885 at 437.298 kb on - strand, within RPSI07_RS09885 at 437.339 kb on + strand, within RPSI07_RS09885 at 437.339 kb on + strand, within RPSI07_RS09885 at 437.385 kb on - strand, within RPSI07_RS09885 at 437.385 kb on - strand, within RPSI07_RS09885 at 437.693 kb on + strand, within RPSI07_RS09885 at 437.694 kb on - strand, within RPSI07_RS09885 at 437.800 kb on + strand, within RPSI07_RS09885 at 437.801 kb on + strand, within RPSI07_RS09885 at 437.801 kb on + strand, within RPSI07_RS09885 at 437.801 kb on + strand, within RPSI07_RS09885 at 437.801 kb on + strand, within RPSI07_RS09885 at 437.802 kb on - strand, within RPSI07_RS09885 at 437.802 kb on - strand, within RPSI07_RS09885 at 437.802 kb on - strand, within RPSI07_RS09885 at 437.802 kb on - strand, within RPSI07_RS09885 at 437.803 kb on + strand, within RPSI07_RS09885 at 437.803 kb on + strand, within RPSI07_RS09885 at 437.803 kb on + strand, within RPSI07_RS09885 at 437.803 kb on + strand, within RPSI07_RS09885 at 437.803 kb on + strand, within RPSI07_RS09885 at 437.804 kb on - strand, within RPSI07_RS09885 at 437.804 kb on - strand, within RPSI07_RS09885 at 437.804 kb on - strand, within RPSI07_RS09885 at 437.804 kb on - strand, within RPSI07_RS09885 at 437.804 kb on - strand, within RPSI07_RS09885 at 437.804 kb on - strand, within RPSI07_RS09885 at 437.804 kb on - strand, within RPSI07_RS09885 at 437.804 kb on - strand, within RPSI07_RS09885 at 437.804 kb on - strand, within RPSI07_RS09885 at 437.805 kb on - strand, within RPSI07_RS09885 at 437.948 kb on + strand, within RPSI07_RS09885 at 437.948 kb on + strand, within RPSI07_RS09885 at 437.951 kb on + strand, within RPSI07_RS09885 at 437.960 kb on + strand, within RPSI07_RS09885 at 438.101 kb on - strand at 438.207 kb on + strand at 438.212 kb on + strand at 438.212 kb on + strand at 438.212 kb on + strand at 438.212 kb on + strand at 438.213 kb on - strand at 438.239 kb on - strand at 438.263 kb on + strand at 438.263 kb on + strand at 438.264 kb on - strand at 438.264 kb on - strand at 438.316 kb on - strand at 438.477 kb on + strand, within RPSI07_RS09890 at 438.478 kb on - strand, within RPSI07_RS09890 at 438.478 kb on - strand, within RPSI07_RS09890 at 438.498 kb on + strand, within RPSI07_RS09890 at 438.498 kb on + strand, within RPSI07_RS09890 at 438.498 kb on + strand, within RPSI07_RS09890 at 438.499 kb on - strand, within RPSI07_RS09890 at 438.499 kb on - strand, within RPSI07_RS09890 at 438.499 kb on - strand, within RPSI07_RS09890 at 438.499 kb on - strand, within RPSI07_RS09890 at 438.499 kb on - strand, within RPSI07_RS09890 at 438.499 kb on - strand, within RPSI07_RS09890 at 438.828 kb on + strand, within RPSI07_RS09890 at 438.829 kb on - strand, within RPSI07_RS09890 at 438.829 kb on - strand, within RPSI07_RS09890 at 438.834 kb on + strand, within RPSI07_RS09890 at 439.076 kb on - strand at 439.076 kb on - strand at 439.076 kb on - strand at 439.076 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol_and_CrystalViolet remove 435,570 + RPSI07_RS09880 0.17 -0.4 435,570 + RPSI07_RS09880 0.17 -1.3 435,570 + RPSI07_RS09880 0.17 -1.2 435,570 + RPSI07_RS09880 0.17 -1.2 435,570 + RPSI07_RS09880 0.17 +1.1 435,571 - RPSI07_RS09880 0.17 +6.6 435,571 - RPSI07_RS09880 0.17 -0.8 435,571 - RPSI07_RS09880 0.17 +0.5 435,834 + RPSI07_RS09880 0.41 +0.3 435,834 + RPSI07_RS09880 0.41 +2.4 435,834 + RPSI07_RS09880 0.41 +3.8 435,834 + RPSI07_RS09880 0.41 -0.9 435,835 - RPSI07_RS09880 0.41 -1.0 435,835 - RPSI07_RS09880 0.41 -1.0 435,835 - RPSI07_RS09880 0.41 -2.0 435,835 - RPSI07_RS09880 0.41 +0.5 435,835 - RPSI07_RS09880 0.41 -1.6 435,835 - RPSI07_RS09880 0.41 -0.9 435,933 + RPSI07_RS09880 0.50 -0.2 435,933 + RPSI07_RS09880 0.50 +0.6 435,933 + RPSI07_RS09880 0.50 +1.4 435,933 + RPSI07_RS09880 0.50 +1.1 435,933 + RPSI07_RS09880 0.50 -2.8 435,934 - RPSI07_RS09880 0.50 -0.0 435,934 - RPSI07_RS09880 0.50 +0.1 435,934 - RPSI07_RS09880 0.50 +2.7 435,934 - RPSI07_RS09880 0.50 +1.5 435,934 - RPSI07_RS09880 0.50 -0.3 436,003 - RPSI07_RS09880 0.56 -0.9 436,003 - RPSI07_RS09880 0.56 -0.7 436,003 - RPSI07_RS09880 0.56 +2.4 436,003 - RPSI07_RS09880 0.56 -2.9 436,224 + RPSI07_RS09880 0.75 -1.2 436,224 + RPSI07_RS09880 0.75 +1.4 436,224 + RPSI07_RS09880 0.75 +0.7 436,224 + RPSI07_RS09880 0.75 -0.7 436,224 + RPSI07_RS09880 0.75 +0.9 436,225 - RPSI07_RS09880 0.76 -2.9 436,225 - RPSI07_RS09880 0.76 -1.8 436,225 - RPSI07_RS09880 0.76 -1.0 436,225 - RPSI07_RS09880 0.76 +0.5 436,225 - RPSI07_RS09880 0.76 -1.6 436,242 + RPSI07_RS09880 0.77 -0.9 436,243 - RPSI07_RS09880 0.77 -0.9 436,260 + RPSI07_RS09880 0.79 -1.0 436,260 + RPSI07_RS09880 0.79 -0.3 436,260 + RPSI07_RS09880 0.79 +1.3 436,261 - RPSI07_RS09880 0.79 +1.9 436,261 - RPSI07_RS09880 0.79 +2.9 436,323 - RPSI07_RS09880 0.84 -1.8 436,563 + +3.8 436,563 + -0.0 436,564 - +3.6 436,564 - +1.0 436,564 - +1.0 436,564 - -1.3 436,564 - +0.1 436,564 - +0.6 436,564 - +1.0 436,564 - -0.3 436,564 - -0.4 436,748 + RPSI07_RS09885 0.11 -1.4 436,748 + RPSI07_RS09885 0.11 -0.3 436,749 - RPSI07_RS09885 0.12 -0.2 436,838 + RPSI07_RS09885 0.17 +1.6 436,838 + RPSI07_RS09885 0.17 -1.6 436,910 + RPSI07_RS09885 0.22 -2.8 436,910 + RPSI07_RS09885 0.22 +2.0 437,042 + RPSI07_RS09885 0.30 -1.2 437,042 + RPSI07_RS09885 0.30 -0.5 437,043 - RPSI07_RS09885 0.30 +1.4 437,120 + RPSI07_RS09885 0.35 -1.5 437,120 + RPSI07_RS09885 0.35 +0.9 437,120 + RPSI07_RS09885 0.35 -1.2 437,120 + RPSI07_RS09885 0.35 -1.3 437,121 - RPSI07_RS09885 0.35 -1.3 437,121 - RPSI07_RS09885 0.35 -3.2 437,184 - RPSI07_RS09885 0.39 +0.4 437,184 - RPSI07_RS09885 0.39 -0.6 437,297 + RPSI07_RS09885 0.46 -0.7 437,298 - RPSI07_RS09885 0.46 -3.7 437,298 - RPSI07_RS09885 0.46 +1.4 437,339 + RPSI07_RS09885 0.49 -1.1 437,339 + RPSI07_RS09885 0.49 +0.4 437,385 - RPSI07_RS09885 0.52 -0.7 437,385 - RPSI07_RS09885 0.52 -2.1 437,693 + RPSI07_RS09885 0.71 +0.1 437,694 - RPSI07_RS09885 0.71 -2.6 437,800 + RPSI07_RS09885 0.78 -0.9 437,801 + RPSI07_RS09885 0.78 -1.9 437,801 + RPSI07_RS09885 0.78 -1.3 437,801 + RPSI07_RS09885 0.78 -1.2 437,801 + RPSI07_RS09885 0.78 -1.2 437,802 - RPSI07_RS09885 0.78 -2.0 437,802 - RPSI07_RS09885 0.78 +0.4 437,802 - RPSI07_RS09885 0.78 -1.6 437,802 - RPSI07_RS09885 0.78 -2.8 437,803 + RPSI07_RS09885 0.78 -1.8 437,803 + RPSI07_RS09885 0.78 +0.6 437,803 + RPSI07_RS09885 0.78 -1.8 437,803 + RPSI07_RS09885 0.78 -0.3 437,803 + RPSI07_RS09885 0.78 -0.2 437,804 - RPSI07_RS09885 0.78 -2.6 437,804 - RPSI07_RS09885 0.78 +0.0 437,804 - RPSI07_RS09885 0.78 +1.4 437,804 - RPSI07_RS09885 0.78 -0.6 437,804 - RPSI07_RS09885 0.78 -1.1 437,804 - RPSI07_RS09885 0.78 -0.2 437,804 - RPSI07_RS09885 0.78 -0.1 437,804 - RPSI07_RS09885 0.78 -1.3 437,804 - RPSI07_RS09885 0.78 -1.2 437,805 - RPSI07_RS09885 0.78 -1.8 437,948 + RPSI07_RS09885 0.88 -1.1 437,948 + RPSI07_RS09885 0.88 +0.2 437,951 + RPSI07_RS09885 0.88 -0.4 437,960 + RPSI07_RS09885 0.88 -2.9 438,101 - +0.5 438,207 + +0.3 438,212 + +0.6 438,212 + -0.3 438,212 + -0.7 438,212 + -1.8 438,213 - +1.8 438,239 - -1.0 438,263 + -1.2 438,263 + +0.4 438,264 - +0.2 438,264 - -0.1 438,316 - +2.4 438,477 + RPSI07_RS09890 0.25 -0.9 438,478 - RPSI07_RS09890 0.25 +1.7 438,478 - RPSI07_RS09890 0.25 +0.4 438,498 + RPSI07_RS09890 0.28 -0.3 438,498 + RPSI07_RS09890 0.28 -3.1 438,498 + RPSI07_RS09890 0.28 +2.0 438,499 - RPSI07_RS09890 0.28 -0.5 438,499 - RPSI07_RS09890 0.28 -0.6 438,499 - RPSI07_RS09890 0.28 -1.1 438,499 - RPSI07_RS09890 0.28 -0.7 438,499 - RPSI07_RS09890 0.28 -1.2 438,499 - RPSI07_RS09890 0.28 +0.8 438,828 + RPSI07_RS09890 0.71 +1.4 438,829 - RPSI07_RS09890 0.71 -0.7 438,829 - RPSI07_RS09890 0.71 +1.3 438,834 + RPSI07_RS09890 0.72 -0.4 439,076 - -0.4 439,076 - +1.1 439,076 - +0.1 439,076 - -1.0
Or see this region's nucleotide sequence