Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol_and_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS02020 and RPSI07_RS02025 are separated by 147 nucleotides RPSI07_RS02025 and RPSI07_RS02030 are separated by 109 nucleotides RPSI07_RS02030 and RPSI07_RS02035 are separated by 41 nucleotides
RPSI07_RS02020: RPSI07_RS02020 - Rrf2 family transcriptional regulator, at 595,635 to 596,066
_RS02020
RPSI07_RS02025: RPSI07_RS02025 - hypothetical protein, at 596,214 to 596,723
_RS02025
RPSI07_RS02030: RPSI07_RS02030 - TolC family protein, at 596,833 to 598,194
_RS02030
RPSI07_RS02035: RPSI07_RS02035 - efflux RND transporter periplasmic adaptor subunit, at 598,236 to 599,408
_RS02035
Position (kb) 596
597
598
599 Strain fitness (log2 ratio) -2
-1
0
1
2 at 596.093 kb on - strand at 596.201 kb on + strand at 596.202 kb on - strand at 596.205 kb on - strand at 596.205 kb on - strand at 596.205 kb on - strand at 596.761 kb on + strand at 596.762 kb on - strand at 596.764 kb on + strand at 596.764 kb on + strand at 596.764 kb on + strand at 596.764 kb on + strand at 596.765 kb on - strand at 596.827 kb on + strand at 596.827 kb on + strand at 596.828 kb on - strand at 597.028 kb on + strand, within RPSI07_RS02030 at 597.404 kb on + strand, within RPSI07_RS02030 at 597.404 kb on + strand, within RPSI07_RS02030 at 597.469 kb on + strand, within RPSI07_RS02030 at 598.081 kb on + strand at 598.618 kb on - strand, within RPSI07_RS02035
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol_and_CrystalViolet remove 596,093 - -0.6 596,201 + +0.2 596,202 - -1.1 596,205 - -0.9 596,205 - -1.6 596,205 - -1.9 596,761 + +0.6 596,762 - +0.5 596,764 + +0.1 596,764 + -0.7 596,764 + -2.4 596,764 + -0.5 596,765 - -0.7 596,827 + -0.9 596,827 + +0.3 596,828 - -1.7 597,028 + RPSI07_RS02030 0.14 -0.4 597,404 + RPSI07_RS02030 0.42 -2.1 597,404 + RPSI07_RS02030 0.42 +0.6 597,469 + RPSI07_RS02030 0.47 -1.5 598,081 + +2.7 598,618 - RPSI07_RS02035 0.33 -0.8
Or see this region's nucleotide sequence