Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol_and_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS00070 and RPSI07_RS00075 are separated by 10 nucleotides RPSI07_RS00075 and RPSI07_RS00080 are separated by 7 nucleotides RPSI07_RS00080 and RPSI07_RS00085 overlap by 4 nucleotides RPSI07_RS00085 and RPSI07_RS00090 are separated by 75 nucleotides
RPSI07_RS00070: RPSI07_RS00070 - branched-chain amino acid ABC transporter permease, at 17,779 to 18,660
_RS00070
RPSI07_RS00075: RPSI07_RS00075 - branched-chain amino acid ABC transporter permease, at 18,671 to 19,570
_RS00075
RPSI07_RS00080: RPSI07_RS00080 - ABC transporter ATP-binding protein, at 19,578 to 20,375
_RS00080
RPSI07_RS00085: RPSI07_RS00085 - ABC transporter ATP-binding protein, at 20,372 to 21,106
_RS00085
RPSI07_RS00090: RPSI07_RS00090 - LysR family transcriptional regulator, at 21,182 to 22,051
_RS00090
Position (kb) 19
20
21 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 18.658 kb on + strand at 18.658 kb on + strand at 18.702 kb on - strand at 18.702 kb on - strand at 18.755 kb on + strand at 18.755 kb on + strand at 18.755 kb on + strand at 18.756 kb on - strand at 18.819 kb on - strand, within RPSI07_RS00075 at 18.944 kb on + strand, within RPSI07_RS00075 at 18.944 kb on + strand, within RPSI07_RS00075 at 19.933 kb on - strand, within RPSI07_RS00080 at 20.032 kb on - strand, within RPSI07_RS00080 at 20.032 kb on - strand, within RPSI07_RS00080 at 20.313 kb on + strand at 20.314 kb on - strand at 20.314 kb on - strand at 20.314 kb on - strand at 20.412 kb on - strand at 20.412 kb on - strand at 20.696 kb on + strand, within RPSI07_RS00085 at 20.696 kb on + strand, within RPSI07_RS00085 at 20.697 kb on - strand, within RPSI07_RS00085 at 20.698 kb on + strand, within RPSI07_RS00085 at 20.698 kb on + strand, within RPSI07_RS00085 at 20.698 kb on + strand, within RPSI07_RS00085 at 20.699 kb on - strand, within RPSI07_RS00085 at 20.699 kb on - strand, within RPSI07_RS00085 at 21.015 kb on - strand, within RPSI07_RS00085 at 21.092 kb on + strand at 21.093 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol_and_CrystalViolet remove 18,658 + +0.5 18,658 + -0.3 18,702 - +0.1 18,702 - -0.9 18,755 + +0.4 18,755 + +0.3 18,755 + +1.7 18,756 - +0.7 18,819 - RPSI07_RS00075 0.16 -0.1 18,944 + RPSI07_RS00075 0.30 +0.7 18,944 + RPSI07_RS00075 0.30 +0.3 19,933 - RPSI07_RS00080 0.44 -0.7 20,032 - RPSI07_RS00080 0.57 -1.9 20,032 - RPSI07_RS00080 0.57 -0.9 20,313 + +0.9 20,314 - +4.6 20,314 - -0.0 20,314 - -0.7 20,412 - +0.2 20,412 - -1.1 20,696 + RPSI07_RS00085 0.44 -0.7 20,696 + RPSI07_RS00085 0.44 -1.5 20,697 - RPSI07_RS00085 0.44 -1.5 20,698 + RPSI07_RS00085 0.44 +0.3 20,698 + RPSI07_RS00085 0.44 -1.0 20,698 + RPSI07_RS00085 0.44 +1.5 20,699 - RPSI07_RS00085 0.44 -2.6 20,699 - RPSI07_RS00085 0.44 -0.8 21,015 - RPSI07_RS00085 0.87 +0.3 21,092 + -0.0 21,093 - -0.5
Or see this region's nucleotide sequence