Strain Fitness in Ralstonia solanacearum PSI07 around RPSI07_RS22520

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol_and_CrystalViolet

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRPSI07_RS22510 and RPSI07_RS22515 are separated by 94 nucleotidesRPSI07_RS22515 and RPSI07_RS22520 are separated by 73 nucleotidesRPSI07_RS22520 and RPSI07_RS22525 are separated by 25 nucleotides RPSI07_RS22510: RPSI07_RS22510 - O-acetyl-ADP-ribose deacetylase, at 3,204,730 to 3,205,245 _RS22510 RPSI07_RS22515: RPSI07_RS22515 - membrane protein, at 3,205,340 to 3,205,894 _RS22515 RPSI07_RS22520: RPSI07_RS22520 - sigma-54-dependent Fis family transcriptional regulator, at 3,205,968 to 3,207,284 _RS22520 RPSI07_RS22525: RPSI07_RS22525 - sensor histidine kinase, at 3,207,310 to 3,209,307 _RS22525 Position (kb) 3205 3206 3207 3208Strain fitness (log2 ratio) -2 -1 0 1 2at 3205.081 kb on - strand, within RPSI07_RS22510at 3205.102 kb on + strand, within RPSI07_RS22510at 3205.102 kb on + strand, within RPSI07_RS22510at 3205.102 kb on + strand, within RPSI07_RS22510at 3205.102 kb on + strand, within RPSI07_RS22510at 3205.103 kb on - strand, within RPSI07_RS22510at 3205.217 kb on - strandat 3205.217 kb on - strandat 3205.296 kb on + strandat 3205.297 kb on - strandat 3205.297 kb on - strandat 3205.297 kb on - strandat 3205.616 kb on + strand, within RPSI07_RS22515at 3205.616 kb on + strand, within RPSI07_RS22515at 3205.617 kb on - strand, within RPSI07_RS22515at 3205.617 kb on - strand, within RPSI07_RS22515at 3205.617 kb on - strand, within RPSI07_RS22515at 3205.667 kb on + strand, within RPSI07_RS22515at 3205.667 kb on + strand, within RPSI07_RS22515at 3205.667 kb on + strand, within RPSI07_RS22515at 3205.667 kb on + strand, within RPSI07_RS22515at 3205.682 kb on + strand, within RPSI07_RS22515at 3205.683 kb on - strand, within RPSI07_RS22515at 3205.694 kb on + strand, within RPSI07_RS22515at 3205.826 kb on + strand, within RPSI07_RS22515at 3205.997 kb on - strandat 3206.090 kb on - strandat 3206.375 kb on - strand, within RPSI07_RS22520at 3206.377 kb on + strand, within RPSI07_RS22520at 3206.377 kb on + strand, within RPSI07_RS22520at 3206.378 kb on - strand, within RPSI07_RS22520at 3206.378 kb on - strand, within RPSI07_RS22520at 3207.184 kb on + strandat 3207.184 kb on + strandat 3207.184 kb on + strandat 3207.585 kb on + strand, within RPSI07_RS22525at 3207.585 kb on + strand, within RPSI07_RS22525at 3208.163 kb on + strand, within RPSI07_RS22525at 3208.164 kb on - strand, within RPSI07_RS22525at 3208.228 kb on + strand, within RPSI07_RS22525at 3208.229 kb on + strand, within RPSI07_RS22525at 3208.230 kb on - strand, within RPSI07_RS22525

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol_and_CrystalViolet
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3,205,081 - RPSI07_RS22510 0.68 +1.3
3,205,102 + RPSI07_RS22510 0.72 +1.8
3,205,102 + RPSI07_RS22510 0.72 +0.6
3,205,102 + RPSI07_RS22510 0.72 -1.9
3,205,102 + RPSI07_RS22510 0.72 +0.8
3,205,103 - RPSI07_RS22510 0.72 -0.4
3,205,217 - +1.4
3,205,217 - -1.7
3,205,296 + -1.2
3,205,297 - -0.4
3,205,297 - -0.2
3,205,297 - +1.4
3,205,616 + RPSI07_RS22515 0.50 -1.4
3,205,616 + RPSI07_RS22515 0.50 +1.4
3,205,617 - RPSI07_RS22515 0.50 -0.6
3,205,617 - RPSI07_RS22515 0.50 -0.6
3,205,617 - RPSI07_RS22515 0.50 -1.4
3,205,667 + RPSI07_RS22515 0.59 -1.2
3,205,667 + RPSI07_RS22515 0.59 -1.2
3,205,667 + RPSI07_RS22515 0.59 -0.6
3,205,667 + RPSI07_RS22515 0.59 -2.0
3,205,682 + RPSI07_RS22515 0.62 -1.9
3,205,683 - RPSI07_RS22515 0.62 -1.6
3,205,694 + RPSI07_RS22515 0.64 -0.2
3,205,826 + RPSI07_RS22515 0.88 -1.4
3,205,997 - +1.2
3,206,090 - -2.3
3,206,375 - RPSI07_RS22520 0.31 -1.4
3,206,377 + RPSI07_RS22520 0.31 +0.4
3,206,377 + RPSI07_RS22520 0.31 +0.7
3,206,378 - RPSI07_RS22520 0.31 -0.6
3,206,378 - RPSI07_RS22520 0.31 -0.1
3,207,184 + +2.0
3,207,184 + -0.3
3,207,184 + -2.1
3,207,585 + RPSI07_RS22525 0.14 -0.4
3,207,585 + RPSI07_RS22525 0.14 -0.7
3,208,163 + RPSI07_RS22525 0.43 -0.3
3,208,164 - RPSI07_RS22525 0.43 -0.9
3,208,228 + RPSI07_RS22525 0.46 -0.2
3,208,229 + RPSI07_RS22525 0.46 -1.4
3,208,230 - RPSI07_RS22525 0.46 -0.6

Or see this region's nucleotide sequence