Strain Fitness in Ralstonia solanacearum PSI07 around RPSI07_RS22515

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol_and_CrystalViolet

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRPSI07_RS22505 and RPSI07_RS22510 are separated by 91 nucleotidesRPSI07_RS22510 and RPSI07_RS22515 are separated by 94 nucleotidesRPSI07_RS22515 and RPSI07_RS22520 are separated by 73 nucleotides RPSI07_RS22505: RPSI07_RS22505 - hypothetical protein, at 3,204,069 to 3,204,638 _RS22505 RPSI07_RS22510: RPSI07_RS22510 - O-acetyl-ADP-ribose deacetylase, at 3,204,730 to 3,205,245 _RS22510 RPSI07_RS22515: RPSI07_RS22515 - membrane protein, at 3,205,340 to 3,205,894 _RS22515 RPSI07_RS22520: RPSI07_RS22520 - sigma-54-dependent Fis family transcriptional regulator, at 3,205,968 to 3,207,284 _RS22520 Position (kb) 3205 3206Strain fitness (log2 ratio) -2 -1 0 1 2at 3204.508 kb on + strand, within RPSI07_RS22505at 3204.509 kb on - strandat 3204.520 kb on + strand, within RPSI07_RS22505at 3204.520 kb on + strand, within RPSI07_RS22505at 3204.545 kb on + strand, within RPSI07_RS22505at 3204.545 kb on + strand, within RPSI07_RS22505at 3204.546 kb on - strand, within RPSI07_RS22505at 3204.546 kb on - strand, within RPSI07_RS22505at 3204.557 kb on - strand, within RPSI07_RS22505at 3204.678 kb on + strandat 3204.678 kb on + strandat 3204.678 kb on + strandat 3204.678 kb on + strandat 3204.678 kb on + strandat 3204.679 kb on - strandat 3204.679 kb on - strandat 3204.679 kb on - strandat 3204.699 kb on + strandat 3204.700 kb on - strandat 3204.700 kb on - strandat 3204.700 kb on - strandat 3204.700 kb on - strandat 3204.741 kb on + strandat 3204.955 kb on + strand, within RPSI07_RS22510at 3204.955 kb on + strand, within RPSI07_RS22510at 3204.955 kb on + strand, within RPSI07_RS22510at 3204.955 kb on + strand, within RPSI07_RS22510at 3204.956 kb on - strand, within RPSI07_RS22510at 3205.081 kb on - strand, within RPSI07_RS22510at 3205.102 kb on + strand, within RPSI07_RS22510at 3205.102 kb on + strand, within RPSI07_RS22510at 3205.102 kb on + strand, within RPSI07_RS22510at 3205.102 kb on + strand, within RPSI07_RS22510at 3205.103 kb on - strand, within RPSI07_RS22510at 3205.217 kb on - strandat 3205.217 kb on - strandat 3205.296 kb on + strandat 3205.297 kb on - strandat 3205.297 kb on - strandat 3205.297 kb on - strandat 3205.616 kb on + strand, within RPSI07_RS22515at 3205.616 kb on + strand, within RPSI07_RS22515at 3205.617 kb on - strand, within RPSI07_RS22515at 3205.617 kb on - strand, within RPSI07_RS22515at 3205.617 kb on - strand, within RPSI07_RS22515at 3205.667 kb on + strand, within RPSI07_RS22515at 3205.667 kb on + strand, within RPSI07_RS22515at 3205.667 kb on + strand, within RPSI07_RS22515at 3205.667 kb on + strand, within RPSI07_RS22515at 3205.682 kb on + strand, within RPSI07_RS22515at 3205.683 kb on - strand, within RPSI07_RS22515at 3205.694 kb on + strand, within RPSI07_RS22515at 3205.826 kb on + strand, within RPSI07_RS22515at 3205.997 kb on - strandat 3206.090 kb on - strandat 3206.375 kb on - strand, within RPSI07_RS22520at 3206.377 kb on + strand, within RPSI07_RS22520at 3206.377 kb on + strand, within RPSI07_RS22520at 3206.378 kb on - strand, within RPSI07_RS22520at 3206.378 kb on - strand, within RPSI07_RS22520

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol_and_CrystalViolet
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3,204,508 + RPSI07_RS22505 0.77 +0.8
3,204,509 - -0.2
3,204,520 + RPSI07_RS22505 0.79 +0.5
3,204,520 + RPSI07_RS22505 0.79 -0.5
3,204,545 + RPSI07_RS22505 0.84 +0.8
3,204,545 + RPSI07_RS22505 0.84 +1.4
3,204,546 - RPSI07_RS22505 0.84 -0.3
3,204,546 - RPSI07_RS22505 0.84 -1.6
3,204,557 - RPSI07_RS22505 0.86 +0.1
3,204,678 + +1.0
3,204,678 + -0.2
3,204,678 + -0.2
3,204,678 + +0.4
3,204,678 + +0.8
3,204,679 - -0.5
3,204,679 - -0.9
3,204,679 - +0.1
3,204,699 + -1.6
3,204,700 - +0.2
3,204,700 - +0.4
3,204,700 - +0.1
3,204,700 - +0.2
3,204,741 + -2.3
3,204,955 + RPSI07_RS22510 0.44 -0.6
3,204,955 + RPSI07_RS22510 0.44 +0.1
3,204,955 + RPSI07_RS22510 0.44 +0.1
3,204,955 + RPSI07_RS22510 0.44 -0.8
3,204,956 - RPSI07_RS22510 0.44 -1.0
3,205,081 - RPSI07_RS22510 0.68 +1.3
3,205,102 + RPSI07_RS22510 0.72 +1.8
3,205,102 + RPSI07_RS22510 0.72 +0.6
3,205,102 + RPSI07_RS22510 0.72 -1.9
3,205,102 + RPSI07_RS22510 0.72 +0.8
3,205,103 - RPSI07_RS22510 0.72 -0.4
3,205,217 - +1.4
3,205,217 - -1.7
3,205,296 + -1.2
3,205,297 - -0.4
3,205,297 - -0.2
3,205,297 - +1.4
3,205,616 + RPSI07_RS22515 0.50 -1.4
3,205,616 + RPSI07_RS22515 0.50 +1.4
3,205,617 - RPSI07_RS22515 0.50 -0.6
3,205,617 - RPSI07_RS22515 0.50 -0.6
3,205,617 - RPSI07_RS22515 0.50 -1.4
3,205,667 + RPSI07_RS22515 0.59 -1.2
3,205,667 + RPSI07_RS22515 0.59 -1.2
3,205,667 + RPSI07_RS22515 0.59 -0.6
3,205,667 + RPSI07_RS22515 0.59 -2.0
3,205,682 + RPSI07_RS22515 0.62 -1.9
3,205,683 - RPSI07_RS22515 0.62 -1.6
3,205,694 + RPSI07_RS22515 0.64 -0.2
3,205,826 + RPSI07_RS22515 0.88 -1.4
3,205,997 - +1.2
3,206,090 - -2.3
3,206,375 - RPSI07_RS22520 0.31 -1.4
3,206,377 + RPSI07_RS22520 0.31 +0.4
3,206,377 + RPSI07_RS22520 0.31 +0.7
3,206,378 - RPSI07_RS22520 0.31 -0.6
3,206,378 - RPSI07_RS22520 0.31 -0.1

Or see this region's nucleotide sequence