Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol_and_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS20315 and RPSI07_RS20320 overlap by 4 nucleotides RPSI07_RS20320 and RPSI07_RS20325 are separated by 69 nucleotides RPSI07_RS20325 and RPSI07_RS20330 are separated by 399 nucleotides
RPSI07_RS20315: RPSI07_RS20315 - protein TolQ, at 2,736,757 to 2,737,449
_RS20315
RPSI07_RS20320: RPSI07_RS20320 - tol-pal system-associated acyl-CoA thioesterase, at 2,737,446 to 2,737,850
_RS20320
RPSI07_RS20325: RPSI07_RS20325 - NAD(P)-dependent oxidoreductase, at 2,737,920 to 2,738,672
_RS20325
RPSI07_RS20330: RPSI07_RS20330 - serine hydroxymethyltransferase, at 2,739,072 to 2,740,319
_RS20330
Position (kb)
2737
2738
2739 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2737.008 kb on + strand, within RPSI07_RS20315 at 2737.009 kb on - strand, within RPSI07_RS20315 at 2737.009 kb on - strand, within RPSI07_RS20315 at 2737.009 kb on - strand, within RPSI07_RS20315 at 2737.009 kb on - strand, within RPSI07_RS20315 at 2737.010 kb on + strand, within RPSI07_RS20315 at 2737.010 kb on + strand, within RPSI07_RS20315 at 2737.010 kb on + strand, within RPSI07_RS20315 at 2737.010 kb on + strand, within RPSI07_RS20315 at 2737.010 kb on + strand, within RPSI07_RS20315 at 2737.011 kb on - strand, within RPSI07_RS20315 at 2737.011 kb on - strand, within RPSI07_RS20315 at 2737.235 kb on + strand, within RPSI07_RS20315 at 2737.235 kb on + strand, within RPSI07_RS20315 at 2737.307 kb on - strand, within RPSI07_RS20315 at 2737.331 kb on + strand, within RPSI07_RS20315 at 2737.331 kb on + strand, within RPSI07_RS20315 at 2737.331 kb on + strand, within RPSI07_RS20315 at 2737.331 kb on + strand, within RPSI07_RS20315 at 2737.463 kb on + strand at 2737.774 kb on + strand, within RPSI07_RS20320 at 2737.774 kb on + strand, within RPSI07_RS20320 at 2737.809 kb on - strand, within RPSI07_RS20320 at 2737.819 kb on + strand at 2737.819 kb on + strand at 2737.819 kb on + strand at 2737.871 kb on + strand at 2737.946 kb on + strand at 2738.023 kb on + strand, within RPSI07_RS20325 at 2738.024 kb on - strand, within RPSI07_RS20325 at 2738.024 kb on - strand, within RPSI07_RS20325 at 2738.071 kb on + strand, within RPSI07_RS20325 at 2738.071 kb on + strand, within RPSI07_RS20325 at 2738.234 kb on - strand, within RPSI07_RS20325 at 2738.234 kb on - strand, within RPSI07_RS20325 at 2738.257 kb on + strand, within RPSI07_RS20325 at 2738.257 kb on + strand, within RPSI07_RS20325 at 2738.257 kb on + strand, within RPSI07_RS20325 at 2738.258 kb on - strand, within RPSI07_RS20325 at 2738.354 kb on + strand, within RPSI07_RS20325 at 2738.354 kb on + strand, within RPSI07_RS20325 at 2738.664 kb on + strand at 2738.664 kb on + strand at 2738.696 kb on - strand at 2738.794 kb on - strand at 2739.267 kb on + strand, within RPSI07_RS20330 at 2739.660 kb on + strand, within RPSI07_RS20330 at 2739.660 kb on + strand, within RPSI07_RS20330
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol_and_CrystalViolet remove 2,737,008 + RPSI07_RS20315 0.36 -0.7 2,737,009 - RPSI07_RS20315 0.36 -1.3 2,737,009 - RPSI07_RS20315 0.36 +0.3 2,737,009 - RPSI07_RS20315 0.36 +0.5 2,737,009 - RPSI07_RS20315 0.36 -0.5 2,737,010 + RPSI07_RS20315 0.37 -0.8 2,737,010 + RPSI07_RS20315 0.37 -1.0 2,737,010 + RPSI07_RS20315 0.37 -0.8 2,737,010 + RPSI07_RS20315 0.37 -1.3 2,737,010 + RPSI07_RS20315 0.37 +0.7 2,737,011 - RPSI07_RS20315 0.37 -2.0 2,737,011 - RPSI07_RS20315 0.37 -1.1 2,737,235 + RPSI07_RS20315 0.69 +2.5 2,737,235 + RPSI07_RS20315 0.69 -3.4 2,737,307 - RPSI07_RS20315 0.79 -1.0 2,737,331 + RPSI07_RS20315 0.83 -1.5 2,737,331 + RPSI07_RS20315 0.83 +1.6 2,737,331 + RPSI07_RS20315 0.83 +2.3 2,737,331 + RPSI07_RS20315 0.83 -1.5 2,737,463 + -0.5 2,737,774 + RPSI07_RS20320 0.81 -1.3 2,737,774 + RPSI07_RS20320 0.81 -1.0 2,737,809 - RPSI07_RS20320 0.90 -1.5 2,737,819 + +0.7 2,737,819 + -0.8 2,737,819 + -2.4 2,737,871 + -0.7 2,737,946 + +0.8 2,738,023 + RPSI07_RS20325 0.14 -0.6 2,738,024 - RPSI07_RS20325 0.14 -0.9 2,738,024 - RPSI07_RS20325 0.14 -0.9 2,738,071 + RPSI07_RS20325 0.20 -0.0 2,738,071 + RPSI07_RS20325 0.20 +0.9 2,738,234 - RPSI07_RS20325 0.42 +0.8 2,738,234 - RPSI07_RS20325 0.42 -0.2 2,738,257 + RPSI07_RS20325 0.45 +0.4 2,738,257 + RPSI07_RS20325 0.45 +0.3 2,738,257 + RPSI07_RS20325 0.45 +2.6 2,738,258 - RPSI07_RS20325 0.45 -0.9 2,738,354 + RPSI07_RS20325 0.58 +1.1 2,738,354 + RPSI07_RS20325 0.58 -1.3 2,738,664 + -1.7 2,738,664 + +1.1 2,738,696 - +0.8 2,738,794 - -0.6 2,739,267 + RPSI07_RS20330 0.16 +1.3 2,739,660 + RPSI07_RS20330 0.47 +1.3 2,739,660 + RPSI07_RS20330 0.47 -1.0
Or see this region's nucleotide sequence