Strain Fitness in Ralstonia solanacearum PSI07 around RPSI07_RS15785

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol_and_CrystalViolet

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRPSI07_RS15775 and RPSI07_RS15780 are separated by 24 nucleotidesRPSI07_RS15780 and RPSI07_RS15785 are separated by 16 nucleotidesRPSI07_RS15785 and RPSI07_RS15790 are separated by 149 nucleotides RPSI07_RS15775: RPSI07_RS15775 - 4-hydroxy-2-oxoheptanedioate aldolase, at 1,695,173 to 1,695,979 _RS15775 RPSI07_RS15780: RPSI07_RS15780 - homoprotocatechuate degradation operon regulator HpaR, at 1,696,004 to 1,696,468 _RS15780 RPSI07_RS15785: RPSI07_RS15785 - exodeoxyribonuclease III, at 1,696,485 to 1,697,294 _RS15785 RPSI07_RS15790: RPSI07_RS15790 - cytosine deaminase, at 1,697,444 to 1,698,715 _RS15790 Position (kb) 1696 1697 1698Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 1695.581 kb on + strand, within RPSI07_RS15775at 1695.581 kb on + strand, within RPSI07_RS15775at 1695.582 kb on - strand, within RPSI07_RS15775at 1695.582 kb on - strand, within RPSI07_RS15775at 1695.845 kb on + strand, within RPSI07_RS15775at 1695.846 kb on - strand, within RPSI07_RS15775at 1695.846 kb on - strand, within RPSI07_RS15775at 1695.846 kb on - strand, within RPSI07_RS15775at 1695.974 kb on + strandat 1695.974 kb on + strandat 1695.975 kb on - strandat 1695.975 kb on - strandat 1696.518 kb on - strandat 1696.984 kb on + strand, within RPSI07_RS15785at 1696.984 kb on + strand, within RPSI07_RS15785at 1696.984 kb on + strand, within RPSI07_RS15785at 1696.984 kb on + strand, within RPSI07_RS15785at 1696.985 kb on - strand, within RPSI07_RS15785at 1696.985 kb on - strand, within RPSI07_RS15785at 1696.985 kb on - strand, within RPSI07_RS15785at 1697.096 kb on - strand, within RPSI07_RS15785at 1697.126 kb on - strand, within RPSI07_RS15785at 1697.360 kb on + strandat 1697.411 kb on - strandat 1698.103 kb on + strand, within RPSI07_RS15790at 1698.194 kb on + strand, within RPSI07_RS15790at 1698.194 kb on + strand, within RPSI07_RS15790at 1698.194 kb on + strand, within RPSI07_RS15790at 1698.194 kb on + strand, within RPSI07_RS15790at 1698.194 kb on + strand, within RPSI07_RS15790at 1698.194 kb on + strand, within RPSI07_RS15790at 1698.195 kb on - strand, within RPSI07_RS15790at 1698.195 kb on - strand, within RPSI07_RS15790at 1698.195 kb on - strand, within RPSI07_RS15790at 1698.195 kb on - strand, within RPSI07_RS15790at 1698.195 kb on - strand, within RPSI07_RS15790at 1698.195 kb on - strand, within RPSI07_RS15790at 1698.197 kb on + strand, within RPSI07_RS15790at 1698.197 kb on + strand, within RPSI07_RS15790at 1698.197 kb on + strand, within RPSI07_RS15790at 1698.197 kb on + strand, within RPSI07_RS15790at 1698.197 kb on + strand, within RPSI07_RS15790at 1698.198 kb on - strand, within RPSI07_RS15790

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol_and_CrystalViolet
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1,695,581 + RPSI07_RS15775 0.51 -0.1
1,695,581 + RPSI07_RS15775 0.51 +1.9
1,695,582 - RPSI07_RS15775 0.51 -0.7
1,695,582 - RPSI07_RS15775 0.51 -0.7
1,695,845 + RPSI07_RS15775 0.83 +0.3
1,695,846 - RPSI07_RS15775 0.83 +3.1
1,695,846 - RPSI07_RS15775 0.83 +0.8
1,695,846 - RPSI07_RS15775 0.83 +0.3
1,695,974 + +0.1
1,695,974 + -2.7
1,695,975 - -0.7
1,695,975 - +1.0
1,696,518 - +1.1
1,696,984 + RPSI07_RS15785 0.62 +0.1
1,696,984 + RPSI07_RS15785 0.62 +0.4
1,696,984 + RPSI07_RS15785 0.62 -2.9
1,696,984 + RPSI07_RS15785 0.62 -1.4
1,696,985 - RPSI07_RS15785 0.62 -0.9
1,696,985 - RPSI07_RS15785 0.62 -0.4
1,696,985 - RPSI07_RS15785 0.62 -0.4
1,697,096 - RPSI07_RS15785 0.75 +2.1
1,697,126 - RPSI07_RS15785 0.79 +1.6
1,697,360 + +1.1
1,697,411 - +0.1
1,698,103 + RPSI07_RS15790 0.52 -0.6
1,698,194 + RPSI07_RS15790 0.59 -1.1
1,698,194 + RPSI07_RS15790 0.59 +1.4
1,698,194 + RPSI07_RS15790 0.59 -0.1
1,698,194 + RPSI07_RS15790 0.59 +0.6
1,698,194 + RPSI07_RS15790 0.59 -0.7
1,698,194 + RPSI07_RS15790 0.59 -2.4
1,698,195 - RPSI07_RS15790 0.59 -1.2
1,698,195 - RPSI07_RS15790 0.59 -0.6
1,698,195 - RPSI07_RS15790 0.59 -0.2
1,698,195 - RPSI07_RS15790 0.59 +0.7
1,698,195 - RPSI07_RS15790 0.59 +0.7
1,698,195 - RPSI07_RS15790 0.59 -0.1
1,698,197 + RPSI07_RS15790 0.59 -0.5
1,698,197 + RPSI07_RS15790 0.59 -1.1
1,698,197 + RPSI07_RS15790 0.59 +0.0
1,698,197 + RPSI07_RS15790 0.59 -0.7
1,698,197 + RPSI07_RS15790 0.59 -2.1
1,698,198 - RPSI07_RS15790 0.59 -0.9

Or see this region's nucleotide sequence