Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol_and_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS10620 and RPSI07_RS10625 are separated by 19 nucleotides RPSI07_RS10625 and RPSI07_RS10630 are separated by 145 nucleotides
RPSI07_RS10620: RPSI07_RS10620 - UbiX family flavin prenyltransferase, at 586,509 to 587,165
_RS10620
RPSI07_RS10625: RPSI07_RS10625 - monothiol glutaredoxin, Grx4 family, at 587,185 to 587,496
_RS10625
RPSI07_RS10630: RPSI07_RS10630 - group II intron reverse transcriptase/maturase, at 587,642 to 589,339
_RS10630
Position (kb) 587
588
589
590 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5
6 at 587.223 kb on - strand, within RPSI07_RS10625 at 587.223 kb on - strand, within RPSI07_RS10625 at 587.587 kb on - strand at 587.587 kb on - strand at 587.595 kb on - strand at 587.595 kb on - strand at 587.595 kb on - strand at 587.595 kb on - strand at 587.615 kb on - strand at 587.630 kb on - strand at 587.644 kb on - strand at 587.644 kb on - strand at 587.644 kb on - strand at 587.724 kb on - strand at 587.724 kb on - strand at 587.744 kb on + strand at 587.745 kb on - strand at 587.750 kb on + strand at 587.832 kb on + strand, within RPSI07_RS10630 at 587.833 kb on - strand, within RPSI07_RS10630 at 587.833 kb on - strand, within RPSI07_RS10630 at 587.839 kb on + strand, within RPSI07_RS10630 at 587.839 kb on + strand, within RPSI07_RS10630 at 587.931 kb on + strand, within RPSI07_RS10630 at 587.931 kb on + strand, within RPSI07_RS10630 at 587.931 kb on + strand, within RPSI07_RS10630 at 587.931 kb on + strand, within RPSI07_RS10630 at 587.932 kb on - strand, within RPSI07_RS10630 at 587.982 kb on + strand, within RPSI07_RS10630 at 588.066 kb on + strand, within RPSI07_RS10630 at 588.067 kb on - strand, within RPSI07_RS10630 at 588.140 kb on - strand, within RPSI07_RS10630 at 588.189 kb on + strand, within RPSI07_RS10630 at 588.254 kb on - strand, within RPSI07_RS10630 at 588.336 kb on + strand, within RPSI07_RS10630 at 588.337 kb on - strand, within RPSI07_RS10630 at 588.507 kb on + strand, within RPSI07_RS10630 at 588.508 kb on - strand, within RPSI07_RS10630 at 588.575 kb on + strand, within RPSI07_RS10630 at 588.575 kb on + strand, within RPSI07_RS10630 at 588.575 kb on + strand, within RPSI07_RS10630 at 588.576 kb on - strand, within RPSI07_RS10630 at 588.576 kb on - strand, within RPSI07_RS10630 at 588.576 kb on - strand, within RPSI07_RS10630 at 588.677 kb on + strand, within RPSI07_RS10630 at 588.755 kb on - strand, within RPSI07_RS10630 at 588.828 kb on - strand, within RPSI07_RS10630 at 588.828 kb on - strand, within RPSI07_RS10630 at 588.828 kb on - strand, within RPSI07_RS10630 at 588.873 kb on + strand, within RPSI07_RS10630 at 588.874 kb on - strand, within RPSI07_RS10630 at 588.924 kb on + strand, within RPSI07_RS10630 at 588.924 kb on + strand, within RPSI07_RS10630 at 588.924 kb on + strand, within RPSI07_RS10630 at 588.924 kb on + strand, within RPSI07_RS10630 at 588.924 kb on + strand, within RPSI07_RS10630 at 588.925 kb on - strand, within RPSI07_RS10630 at 588.926 kb on + strand, within RPSI07_RS10630 at 588.926 kb on + strand, within RPSI07_RS10630 at 588.926 kb on + strand, within RPSI07_RS10630 at 588.926 kb on + strand, within RPSI07_RS10630 at 588.927 kb on - strand, within RPSI07_RS10630 at 588.927 kb on - strand, within RPSI07_RS10630 at 588.927 kb on - strand, within RPSI07_RS10630 at 588.927 kb on - strand, within RPSI07_RS10630 at 588.965 kb on + strand, within RPSI07_RS10630 at 588.998 kb on - strand, within RPSI07_RS10630 at 588.999 kb on + strand, within RPSI07_RS10630 at 588.999 kb on + strand, within RPSI07_RS10630 at 588.999 kb on + strand, within RPSI07_RS10630 at 588.999 kb on + strand, within RPSI07_RS10630 at 588.999 kb on - strand, within RPSI07_RS10630 at 589.000 kb on - strand, within RPSI07_RS10630 at 589.010 kb on + strand, within RPSI07_RS10630 at 589.136 kb on + strand, within RPSI07_RS10630 at 589.232 kb on + strand at 589.232 kb on + strand at 589.233 kb on - strand at 589.233 kb on - strand at 589.289 kb on - strand at 589.289 kb on - strand at 589.289 kb on - strand at 589.498 kb on + strand at 589.557 kb on + strand at 589.623 kb on - strand at 589.630 kb on + strand at 589.630 kb on + strand at 589.631 kb on - strand at 589.631 kb on - strand at 589.631 kb on - strand at 589.732 kb on + strand at 589.774 kb on - strand at 589.777 kb on - strand at 589.824 kb on + strand at 589.824 kb on + strand at 589.825 kb on - strand at 589.825 kb on - strand at 589.898 kb on + strand at 589.898 kb on + strand at 589.898 kb on + strand at 589.899 kb on - strand at 589.899 kb on - strand at 589.899 kb on - strand at 589.919 kb on + strand at 589.957 kb on - strand at 589.962 kb on + strand at 589.963 kb on - strand at 589.963 kb on - strand at 589.971 kb on - strand at 590.019 kb on + strand at 590.206 kb on - strand at 590.256 kb on - strand at 590.256 kb on - strand at 590.281 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol_and_CrystalViolet remove 587,223 - RPSI07_RS10625 0.12 +0.4 587,223 - RPSI07_RS10625 0.12 +2.0 587,587 - +1.4 587,587 - -0.8 587,595 - -0.6 587,595 - -0.6 587,595 - -1.8 587,595 - -1.1 587,615 - +1.4 587,630 - +1.0 587,644 - -1.1 587,644 - +0.2 587,644 - +0.7 587,724 - -0.2 587,724 - +0.2 587,744 + +1.6 587,745 - -1.0 587,750 + +1.1 587,832 + RPSI07_RS10630 0.11 -2.2 587,833 - RPSI07_RS10630 0.11 +0.4 587,833 - RPSI07_RS10630 0.11 +0.5 587,839 + RPSI07_RS10630 0.12 +0.2 587,839 + RPSI07_RS10630 0.12 +0.4 587,931 + RPSI07_RS10630 0.17 -1.2 587,931 + RPSI07_RS10630 0.17 +0.2 587,931 + RPSI07_RS10630 0.17 -0.2 587,931 + RPSI07_RS10630 0.17 -1.6 587,932 - RPSI07_RS10630 0.17 +0.6 587,982 + RPSI07_RS10630 0.20 +1.5 588,066 + RPSI07_RS10630 0.25 -0.8 588,067 - RPSI07_RS10630 0.25 +1.0 588,140 - RPSI07_RS10630 0.29 -1.1 588,189 + RPSI07_RS10630 0.32 -0.0 588,254 - RPSI07_RS10630 0.36 -0.2 588,336 + RPSI07_RS10630 0.41 +1.4 588,337 - RPSI07_RS10630 0.41 -0.7 588,507 + RPSI07_RS10630 0.51 -0.3 588,508 - RPSI07_RS10630 0.51 +1.0 588,575 + RPSI07_RS10630 0.55 +0.4 588,575 + RPSI07_RS10630 0.55 -0.0 588,575 + RPSI07_RS10630 0.55 +1.4 588,576 - RPSI07_RS10630 0.55 -1.6 588,576 - RPSI07_RS10630 0.55 -0.2 588,576 - RPSI07_RS10630 0.55 +0.3 588,677 + RPSI07_RS10630 0.61 +3.6 588,755 - RPSI07_RS10630 0.66 -0.6 588,828 - RPSI07_RS10630 0.70 -0.1 588,828 - RPSI07_RS10630 0.70 -0.7 588,828 - RPSI07_RS10630 0.70 +0.2 588,873 + RPSI07_RS10630 0.72 -1.0 588,874 - RPSI07_RS10630 0.73 -0.6 588,924 + RPSI07_RS10630 0.76 -2.4 588,924 + RPSI07_RS10630 0.76 +1.0 588,924 + RPSI07_RS10630 0.76 +0.3 588,924 + RPSI07_RS10630 0.76 -0.2 588,924 + RPSI07_RS10630 0.76 -0.6 588,925 - RPSI07_RS10630 0.76 +1.3 588,926 + RPSI07_RS10630 0.76 +0.4 588,926 + RPSI07_RS10630 0.76 +1.8 588,926 + RPSI07_RS10630 0.76 -0.4 588,926 + RPSI07_RS10630 0.76 +0.8 588,927 - RPSI07_RS10630 0.76 -0.1 588,927 - RPSI07_RS10630 0.76 +2.5 588,927 - RPSI07_RS10630 0.76 +0.8 588,927 - RPSI07_RS10630 0.76 -0.1 588,965 + RPSI07_RS10630 0.78 -1.2 588,998 - RPSI07_RS10630 0.80 -0.4 588,999 + RPSI07_RS10630 0.80 -2.2 588,999 + RPSI07_RS10630 0.80 -1.2 588,999 + RPSI07_RS10630 0.80 +1.0 588,999 + RPSI07_RS10630 0.80 -0.3 588,999 - RPSI07_RS10630 0.80 +0.4 589,000 - RPSI07_RS10630 0.80 +0.4 589,010 + RPSI07_RS10630 0.81 +2.4 589,136 + RPSI07_RS10630 0.88 +1.1 589,232 + -0.9 589,232 + -0.7 589,233 - -1.3 589,233 - +0.4 589,289 - +3.0 589,289 - +0.5 589,289 - -1.1 589,498 + -0.4 589,557 + +1.4 589,623 - +0.9 589,630 + -2.5 589,630 + -1.1 589,631 - -0.5 589,631 - +2.4 589,631 - -0.6 589,732 + +0.3 589,774 - +0.1 589,777 - +2.2 589,824 + +1.4 589,824 + +1.2 589,825 - -1.4 589,825 - +1.4 589,898 + +6.6 589,898 + +3.0 589,898 + +0.8 589,899 - +1.0 589,899 - -1.7 589,899 - -0.0 589,919 + +0.8 589,957 - -1.1 589,962 + -0.4 589,963 - -1.2 589,963 - -1.9 589,971 - +0.2 590,019 + +0.8 590,206 - -0.0 590,256 - +0.6 590,256 - -1.4 590,281 + +1.4
Or see this region's nucleotide sequence