Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol_and_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS10540 and RPSI07_RS10545 are separated by 531 nucleotides RPSI07_RS10545 and RPSI07_RS10550 are separated by 72 nucleotides
RPSI07_RS10540: RPSI07_RS10540 - TonB-denpendent receptor, at 564,826 to 566,925
_RS10540
RPSI07_RS10545: RPSI07_RS10545 - gamma-glutamyltransferase family protein, at 567,457 to 569,325
_RS10545
RPSI07_RS10550: RPSI07_RS10550 - TonB-dependent siderophore receptor, at 569,398 to 571,803
_RS10550
Position (kb) 567
568
569
570 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 566.477 kb on + strand, within RPSI07_RS10540 at 566.477 kb on + strand, within RPSI07_RS10540 at 566.478 kb on - strand, within RPSI07_RS10540 at 566.478 kb on - strand, within RPSI07_RS10540 at 566.492 kb on + strand, within RPSI07_RS10540 at 566.492 kb on + strand, within RPSI07_RS10540 at 566.492 kb on + strand, within RPSI07_RS10540 at 566.541 kb on - strand, within RPSI07_RS10540 at 566.541 kb on - strand, within RPSI07_RS10540 at 566.639 kb on + strand, within RPSI07_RS10540 at 566.640 kb on - strand, within RPSI07_RS10540 at 566.753 kb on + strand at 566.753 kb on + strand at 566.754 kb on - strand at 566.967 kb on + strand at 566.967 kb on + strand at 566.967 kb on + strand at 567.065 kb on + strand at 567.065 kb on + strand at 567.065 kb on + strand at 567.065 kb on + strand at 567.065 kb on + strand at 567.066 kb on - strand at 567.066 kb on - strand at 567.066 kb on - strand at 567.066 kb on - strand at 567.761 kb on + strand, within RPSI07_RS10545 at 567.818 kb on - strand, within RPSI07_RS10545 at 567.818 kb on - strand, within RPSI07_RS10545 at 567.845 kb on - strand, within RPSI07_RS10545 at 568.132 kb on + strand, within RPSI07_RS10545 at 568.133 kb on - strand, within RPSI07_RS10545 at 568.240 kb on + strand, within RPSI07_RS10545 at 568.240 kb on + strand, within RPSI07_RS10545 at 568.240 kb on + strand, within RPSI07_RS10545 at 568.240 kb on + strand, within RPSI07_RS10545 at 568.240 kb on + strand, within RPSI07_RS10545 at 568.240 kb on + strand, within RPSI07_RS10545 at 568.241 kb on - strand, within RPSI07_RS10545 at 568.241 kb on - strand, within RPSI07_RS10545 at 568.364 kb on - strand, within RPSI07_RS10545 at 568.364 kb on - strand, within RPSI07_RS10545 at 568.588 kb on + strand, within RPSI07_RS10545 at 568.588 kb on + strand, within RPSI07_RS10545 at 568.588 kb on + strand, within RPSI07_RS10545 at 568.588 kb on + strand, within RPSI07_RS10545 at 568.588 kb on + strand, within RPSI07_RS10545 at 568.589 kb on - strand, within RPSI07_RS10545 at 568.645 kb on + strand, within RPSI07_RS10545 at 568.646 kb on - strand, within RPSI07_RS10545 at 569.044 kb on + strand, within RPSI07_RS10545 at 569.045 kb on - strand, within RPSI07_RS10545 at 569.444 kb on + strand at 569.445 kb on + strand at 569.445 kb on - strand at 569.445 kb on - strand at 569.445 kb on - strand at 569.465 kb on + strand at 569.541 kb on - strand at 569.541 kb on - strand at 569.673 kb on - strand, within RPSI07_RS10550 at 569.673 kb on - strand, within RPSI07_RS10550 at 569.673 kb on - strand, within RPSI07_RS10550 at 569.771 kb on + strand, within RPSI07_RS10550 at 569.771 kb on + strand, within RPSI07_RS10550 at 569.771 kb on + strand, within RPSI07_RS10550 at 569.772 kb on - strand, within RPSI07_RS10550 at 569.772 kb on - strand, within RPSI07_RS10550 at 569.777 kb on + strand, within RPSI07_RS10550 at 569.777 kb on + strand, within RPSI07_RS10550 at 569.778 kb on - strand, within RPSI07_RS10550 at 570.066 kb on - strand, within RPSI07_RS10550 at 570.080 kb on + strand, within RPSI07_RS10550 at 570.092 kb on + strand, within RPSI07_RS10550 at 570.092 kb on + strand, within RPSI07_RS10550 at 570.093 kb on - strand, within RPSI07_RS10550 at 570.093 kb on - strand, within RPSI07_RS10550 at 570.242 kb on + strand, within RPSI07_RS10550
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol_and_CrystalViolet remove 566,477 + RPSI07_RS10540 0.79 -0.8 566,477 + RPSI07_RS10540 0.79 +0.1 566,478 - RPSI07_RS10540 0.79 -0.7 566,478 - RPSI07_RS10540 0.79 +0.3 566,492 + RPSI07_RS10540 0.79 +0.5 566,492 + RPSI07_RS10540 0.79 -0.3 566,492 + RPSI07_RS10540 0.79 -0.3 566,541 - RPSI07_RS10540 0.82 -0.2 566,541 - RPSI07_RS10540 0.82 +1.0 566,639 + RPSI07_RS10540 0.86 -1.3 566,640 - RPSI07_RS10540 0.86 +1.4 566,753 + -0.5 566,753 + -0.9 566,754 - +0.4 566,967 + +0.7 566,967 + -0.9 566,967 + -1.2 567,065 + +0.2 567,065 + +0.9 567,065 + -2.5 567,065 + +0.4 567,065 + +1.0 567,066 - +1.3 567,066 - +0.2 567,066 - +1.5 567,066 - +0.2 567,761 + RPSI07_RS10545 0.16 -1.4 567,818 - RPSI07_RS10545 0.19 +0.4 567,818 - RPSI07_RS10545 0.19 -0.6 567,845 - RPSI07_RS10545 0.21 -0.3 568,132 + RPSI07_RS10545 0.36 -0.1 568,133 - RPSI07_RS10545 0.36 +0.3 568,240 + RPSI07_RS10545 0.42 -0.2 568,240 + RPSI07_RS10545 0.42 -1.3 568,240 + RPSI07_RS10545 0.42 +2.6 568,240 + RPSI07_RS10545 0.42 -1.0 568,240 + RPSI07_RS10545 0.42 -1.9 568,240 + RPSI07_RS10545 0.42 -1.0 568,241 - RPSI07_RS10545 0.42 +0.4 568,241 - RPSI07_RS10545 0.42 -1.4 568,364 - RPSI07_RS10545 0.49 +1.2 568,364 - RPSI07_RS10545 0.49 +0.6 568,588 + RPSI07_RS10545 0.61 -0.2 568,588 + RPSI07_RS10545 0.61 -1.9 568,588 + RPSI07_RS10545 0.61 -1.0 568,588 + RPSI07_RS10545 0.61 +0.4 568,588 + RPSI07_RS10545 0.61 -2.0 568,589 - RPSI07_RS10545 0.61 +0.5 568,645 + RPSI07_RS10545 0.64 -2.0 568,646 - RPSI07_RS10545 0.64 -0.6 569,044 + RPSI07_RS10545 0.85 -0.3 569,045 - RPSI07_RS10545 0.85 +0.4 569,444 + +0.0 569,445 + -0.2 569,445 - -0.7 569,445 - -1.6 569,445 - -0.9 569,465 + +0.4 569,541 - +1.4 569,541 - -1.3 569,673 - RPSI07_RS10550 0.11 +1.4 569,673 - RPSI07_RS10550 0.11 +0.2 569,673 - RPSI07_RS10550 0.11 +0.0 569,771 + RPSI07_RS10550 0.16 -3.5 569,771 + RPSI07_RS10550 0.16 -0.2 569,771 + RPSI07_RS10550 0.16 -0.5 569,772 - RPSI07_RS10550 0.16 +1.0 569,772 - RPSI07_RS10550 0.16 -1.3 569,777 + RPSI07_RS10550 0.16 -2.9 569,777 + RPSI07_RS10550 0.16 -0.5 569,778 - RPSI07_RS10550 0.16 -0.5 570,066 - RPSI07_RS10550 0.28 -1.3 570,080 + RPSI07_RS10550 0.28 +1.1 570,092 + RPSI07_RS10550 0.29 -0.9 570,092 + RPSI07_RS10550 0.29 -2.7 570,093 - RPSI07_RS10550 0.29 +0.9 570,093 - RPSI07_RS10550 0.29 +0.5 570,242 + RPSI07_RS10550 0.35 +0.4
Or see this region's nucleotide sequence