Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol_and_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS10445 and RPSI07_RS10450 are separated by 52 nucleotides RPSI07_RS10450 and RPSI07_RS10455 are separated by 61 nucleotides RPSI07_RS10455 and RPSI07_RS10460 overlap by 8 nucleotides
RPSI07_RS10445: RPSI07_RS10445 - twin-arginine translocase subunit TatB, at 545,333 to 545,851
_RS10445
RPSI07_RS10450: RPSI07_RS10450 - twin-arginine translocase subunit TatC, at 545,904 to 546,701
_RS10450
RPSI07_RS10455: RPSI07_RS10455 - ABC transporter ATP-binding protein, at 546,763 to 547,839
_RS10455
RPSI07_RS10460: RPSI07_RS10460 - iron ABC transporter permease, at 547,832 to 549,643
_RS10460
Position (kb) 546
547
548 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 546.042 kb on + strand, within RPSI07_RS10450 at 546.202 kb on - strand, within RPSI07_RS10450 at 546.235 kb on - strand, within RPSI07_RS10450 at 546.313 kb on - strand, within RPSI07_RS10450 at 546.399 kb on + strand, within RPSI07_RS10450 at 546.523 kb on - strand, within RPSI07_RS10450 at 546.523 kb on - strand, within RPSI07_RS10450 at 546.616 kb on - strand, within RPSI07_RS10450 at 546.616 kb on - strand, within RPSI07_RS10450 at 546.616 kb on - strand, within RPSI07_RS10450 at 546.765 kb on - strand at 546.765 kb on - strand at 546.906 kb on - strand, within RPSI07_RS10455 at 546.906 kb on - strand, within RPSI07_RS10455 at 547.157 kb on + strand, within RPSI07_RS10455 at 547.373 kb on + strand, within RPSI07_RS10455 at 547.373 kb on + strand, within RPSI07_RS10455 at 547.374 kb on - strand, within RPSI07_RS10455 at 547.374 kb on - strand, within RPSI07_RS10455 at 547.471 kb on + strand, within RPSI07_RS10455 at 547.472 kb on - strand, within RPSI07_RS10455 at 547.571 kb on + strand, within RPSI07_RS10455 at 547.992 kb on + strand at 547.992 kb on + strand at 547.992 kb on + strand at 547.993 kb on - strand at 547.993 kb on - strand at 548.181 kb on + strand, within RPSI07_RS10460 at 548.202 kb on + strand, within RPSI07_RS10460 at 548.398 kb on - strand, within RPSI07_RS10460 at 548.458 kb on - strand, within RPSI07_RS10460 at 548.458 kb on - strand, within RPSI07_RS10460 at 548.663 kb on + strand, within RPSI07_RS10460 at 548.663 kb on + strand, within RPSI07_RS10460 at 548.663 kb on + strand, within RPSI07_RS10460 at 548.664 kb on - strand, within RPSI07_RS10460 at 548.664 kb on - strand, within RPSI07_RS10460 at 548.769 kb on + strand, within RPSI07_RS10460 at 548.839 kb on - strand, within RPSI07_RS10460 at 548.839 kb on - strand, within RPSI07_RS10460
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol_and_CrystalViolet remove 546,042 + RPSI07_RS10450 0.17 -0.6 546,202 - RPSI07_RS10450 0.37 -0.1 546,235 - RPSI07_RS10450 0.41 -0.9 546,313 - RPSI07_RS10450 0.51 +0.4 546,399 + RPSI07_RS10450 0.62 -0.1 546,523 - RPSI07_RS10450 0.78 -0.9 546,523 - RPSI07_RS10450 0.78 -1.4 546,616 - RPSI07_RS10450 0.89 -1.6 546,616 - RPSI07_RS10450 0.89 +1.4 546,616 - RPSI07_RS10450 0.89 +0.4 546,765 - -1.0 546,765 - -0.1 546,906 - RPSI07_RS10455 0.13 -0.8 546,906 - RPSI07_RS10455 0.13 -1.7 547,157 + RPSI07_RS10455 0.37 -1.4 547,373 + RPSI07_RS10455 0.57 -0.1 547,373 + RPSI07_RS10455 0.57 +2.9 547,374 - RPSI07_RS10455 0.57 -1.4 547,374 - RPSI07_RS10455 0.57 +1.4 547,471 + RPSI07_RS10455 0.66 +2.4 547,472 - RPSI07_RS10455 0.66 +1.0 547,571 + RPSI07_RS10455 0.75 -0.5 547,992 + -0.9 547,992 + +0.2 547,992 + -0.7 547,993 - -1.6 547,993 - -0.5 548,181 + RPSI07_RS10460 0.19 +0.7 548,202 + RPSI07_RS10460 0.20 +0.9 548,398 - RPSI07_RS10460 0.31 +0.8 548,458 - RPSI07_RS10460 0.35 +0.4 548,458 - RPSI07_RS10460 0.35 -1.0 548,663 + RPSI07_RS10460 0.46 +2.4 548,663 + RPSI07_RS10460 0.46 -2.0 548,663 + RPSI07_RS10460 0.46 +0.5 548,664 - RPSI07_RS10460 0.46 -0.2 548,664 - RPSI07_RS10460 0.46 +0.7 548,769 + RPSI07_RS10460 0.52 +0.8 548,839 - RPSI07_RS10460 0.56 +0.6 548,839 - RPSI07_RS10460 0.56 -1.2
Or see this region's nucleotide sequence