Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol_and_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS08030 and RPSI07_RS08035 are separated by 6 nucleotides RPSI07_RS08035 and RPSI07_RS08040 overlap by 1 nucleotides RPSI07_RS08040 and RPSI07_RS08045 are separated by 244 nucleotides
RPSI07_RS08030: RPSI07_RS08030 - FAD-binding oxidoreductase, at 40,326 to 41,633
_RS08030
RPSI07_RS08035: RPSI07_RS08035 - alpha/beta hydrolase, at 41,640 to 42,419
_RS08035
RPSI07_RS08040: RPSI07_RS08040 - hypothetical protein, at 42,419 to 43,033
_RS08040
RPSI07_RS08045: RPSI07_RS08045 - membrane protein, at 43,278 to 44,063
_RS08045
Position (kb) 42
43
44 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 41.583 kb on + strand at 41.584 kb on - strand at 41.584 kb on - strand at 41.584 kb on - strand at 41.584 kb on - strand at 41.584 kb on - strand at 41.607 kb on + strand at 41.608 kb on - strand at 41.608 kb on - strand at 41.608 kb on - strand at 41.800 kb on + strand, within RPSI07_RS08035 at 41.800 kb on + strand, within RPSI07_RS08035 at 41.941 kb on + strand, within RPSI07_RS08035 at 42.095 kb on + strand, within RPSI07_RS08035 at 42.095 kb on + strand at 42.096 kb on - strand, within RPSI07_RS08035 at 42.096 kb on - strand, within RPSI07_RS08035 at 42.124 kb on + strand, within RPSI07_RS08035 at 42.125 kb on - strand, within RPSI07_RS08035 at 42.125 kb on - strand, within RPSI07_RS08035 at 42.190 kb on + strand, within RPSI07_RS08035 at 42.190 kb on + strand, within RPSI07_RS08035 at 42.190 kb on - strand, within RPSI07_RS08035 at 42.191 kb on - strand, within RPSI07_RS08035 at 42.247 kb on + strand, within RPSI07_RS08035 at 42.248 kb on - strand, within RPSI07_RS08035 at 42.248 kb on - strand, within RPSI07_RS08035 at 42.386 kb on + strand at 42.386 kb on + strand at 42.386 kb on + strand at 42.387 kb on - strand at 42.387 kb on - strand at 42.474 kb on + strand at 42.474 kb on + strand at 43.100 kb on + strand at 43.100 kb on + strand at 43.100 kb on + strand at 43.100 kb on + strand at 43.100 kb on + strand at 43.100 kb on + strand at 43.100 kb on + strand at 43.101 kb on - strand at 43.101 kb on - strand at 43.101 kb on - strand at 43.101 kb on - strand at 43.101 kb on - strand at 43.101 kb on - strand at 43.101 kb on - strand at 43.101 kb on - strand at 43.221 kb on + strand at 43.222 kb on - strand at 43.222 kb on - strand at 43.500 kb on + strand, within RPSI07_RS08045 at 43.500 kb on + strand, within RPSI07_RS08045 at 43.500 kb on + strand, within RPSI07_RS08045 at 43.500 kb on + strand, within RPSI07_RS08045 at 43.515 kb on + strand, within RPSI07_RS08045 at 43.515 kb on + strand, within RPSI07_RS08045 at 43.516 kb on - strand, within RPSI07_RS08045 at 43.644 kb on + strand, within RPSI07_RS08045 at 43.710 kb on + strand, within RPSI07_RS08045 at 43.842 kb on + strand, within RPSI07_RS08045 at 43.843 kb on - strand, within RPSI07_RS08045 at 43.845 kb on + strand, within RPSI07_RS08045 at 43.845 kb on + strand, within RPSI07_RS08045 at 43.845 kb on + strand, within RPSI07_RS08045 at 43.845 kb on + strand, within RPSI07_RS08045 at 43.846 kb on - strand, within RPSI07_RS08045 at 43.846 kb on - strand, within RPSI07_RS08045 at 43.846 kb on - strand, within RPSI07_RS08045 at 43.846 kb on - strand, within RPSI07_RS08045 at 43.846 kb on - strand, within RPSI07_RS08045 at 44.019 kb on + strand at 44.019 kb on + strand at 44.020 kb on - strand at 44.021 kb on + strand at 44.021 kb on + strand at 44.021 kb on + strand at 44.021 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol_and_CrystalViolet remove 41,583 + +0.2 41,584 - +1.3 41,584 - +0.5 41,584 - +2.2 41,584 - -1.6 41,584 - +0.7 41,607 + -2.0 41,608 - -0.1 41,608 - +2.2 41,608 - -0.3 41,800 + RPSI07_RS08035 0.21 +2.0 41,800 + RPSI07_RS08035 0.21 +0.5 41,941 + RPSI07_RS08035 0.39 +1.6 42,095 + RPSI07_RS08035 0.58 -0.1 42,095 + +3.0 42,096 - RPSI07_RS08035 0.58 -0.1 42,096 - RPSI07_RS08035 0.58 -0.4 42,124 + RPSI07_RS08035 0.62 +1.2 42,125 - RPSI07_RS08035 0.62 +0.3 42,125 - RPSI07_RS08035 0.62 +1.8 42,190 + RPSI07_RS08035 0.71 +1.4 42,190 + RPSI07_RS08035 0.71 -0.3 42,190 - RPSI07_RS08035 0.71 +0.4 42,191 - RPSI07_RS08035 0.71 +1.3 42,247 + RPSI07_RS08035 0.78 -1.4 42,248 - RPSI07_RS08035 0.78 +1.3 42,248 - RPSI07_RS08035 0.78 +0.5 42,386 + -2.2 42,386 + -1.1 42,386 + -0.9 42,387 - -0.7 42,387 - -0.9 42,474 + -1.3 42,474 + -0.7 43,100 + -1.8 43,100 + +0.7 43,100 + -0.7 43,100 + -1.1 43,100 + -0.2 43,100 + +1.5 43,100 + -0.1 43,101 - -0.9 43,101 - +1.4 43,101 - +0.5 43,101 - +1.4 43,101 - -0.9 43,101 - +1.4 43,101 - -2.2 43,101 - +0.1 43,221 + -0.1 43,222 - -0.6 43,222 - +0.4 43,500 + RPSI07_RS08045 0.28 -1.7 43,500 + RPSI07_RS08045 0.28 +3.8 43,500 + RPSI07_RS08045 0.28 +0.8 43,500 + RPSI07_RS08045 0.28 -0.4 43,515 + RPSI07_RS08045 0.30 -1.4 43,515 + RPSI07_RS08045 0.30 -2.3 43,516 - RPSI07_RS08045 0.30 +0.7 43,644 + RPSI07_RS08045 0.47 +2.4 43,710 + RPSI07_RS08045 0.55 +2.6 43,842 + RPSI07_RS08045 0.72 -0.7 43,843 - RPSI07_RS08045 0.72 -0.2 43,845 + RPSI07_RS08045 0.72 -2.5 43,845 + RPSI07_RS08045 0.72 +0.3 43,845 + RPSI07_RS08045 0.72 +3.3 43,845 + RPSI07_RS08045 0.72 -0.7 43,846 - RPSI07_RS08045 0.72 -1.0 43,846 - RPSI07_RS08045 0.72 -0.3 43,846 - RPSI07_RS08045 0.72 -0.9 43,846 - RPSI07_RS08045 0.72 +2.2 43,846 - RPSI07_RS08045 0.72 -0.3 44,019 + +0.0 44,019 + -2.6 44,020 - +1.1 44,021 + -0.1 44,021 + -0.2 44,021 + -0.3 44,021 + -0.2
Or see this region's nucleotide sequence