Strain Fitness in Ralstonia solanacearum PSI07 around RPSI07_RS08030

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol_and_CrystalViolet

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRPSI07_RS08020 and RPSI07_RS08025 overlap by 4 nucleotidesRPSI07_RS08025 and RPSI07_RS08030 overlap by 4 nucleotidesRPSI07_RS08030 and RPSI07_RS08035 are separated by 6 nucleotidesRPSI07_RS08035 and RPSI07_RS08040 overlap by 1 nucleotides RPSI07_RS08020: RPSI07_RS08020 - ectoine/hydroxyectoine ABC transporter permease subunit EhuD, at 38,900 to 39,571 _RS08020 RPSI07_RS08025: RPSI07_RS08025 - amino acid ABC transporter ATP-binding protein, at 39,568 to 40,329 _RS08025 RPSI07_RS08030: RPSI07_RS08030 - FAD-binding oxidoreductase, at 40,326 to 41,633 _RS08030 RPSI07_RS08035: RPSI07_RS08035 - alpha/beta hydrolase, at 41,640 to 42,419 _RS08035 RPSI07_RS08040: RPSI07_RS08040 - hypothetical protein, at 42,419 to 43,033 _RS08040 Position (kb) 40 41 42Strain fitness (log2 ratio) -2 -1 0 1 2 3at 39.358 kb on + strand, within RPSI07_RS08020at 39.358 kb on + strand, within RPSI07_RS08020at 39.470 kb on + strand, within RPSI07_RS08020at 39.470 kb on + strand, within RPSI07_RS08020at 39.470 kb on + strand, within RPSI07_RS08020at 39.471 kb on - strand, within RPSI07_RS08020at 39.471 kb on - strand, within RPSI07_RS08020at 39.471 kb on - strand, within RPSI07_RS08020at 39.471 kb on - strand, within RPSI07_RS08020at 39.471 kb on - strand, within RPSI07_RS08020at 39.472 kb on + strand, within RPSI07_RS08020at 39.472 kb on + strand, within RPSI07_RS08020at 39.472 kb on + strand, within RPSI07_RS08020at 39.473 kb on - strand, within RPSI07_RS08020at 39.473 kb on - strand, within RPSI07_RS08020at 39.473 kb on - strand, within RPSI07_RS08020at 39.491 kb on + strand, within RPSI07_RS08020at 39.491 kb on + strand, within RPSI07_RS08020at 39.491 kb on + strand, within RPSI07_RS08020at 39.724 kb on + strand, within RPSI07_RS08025at 39.724 kb on + strand, within RPSI07_RS08025at 39.724 kb on + strand, within RPSI07_RS08025at 39.725 kb on - strand, within RPSI07_RS08025at 39.877 kb on + strand, within RPSI07_RS08025at 39.877 kb on + strand, within RPSI07_RS08025at 39.877 kb on + strand, within RPSI07_RS08025at 39.878 kb on - strand, within RPSI07_RS08025at 40.325 kb on + strandat 40.325 kb on + strandat 40.326 kb on - strandat 40.467 kb on + strand, within RPSI07_RS08030at 40.745 kb on + strand, within RPSI07_RS08030at 40.746 kb on + strand, within RPSI07_RS08030at 40.746 kb on + strand, within RPSI07_RS08030at 40.747 kb on - strand, within RPSI07_RS08030at 40.747 kb on - strand, within RPSI07_RS08030at 40.747 kb on - strand, within RPSI07_RS08030at 40.914 kb on + strand, within RPSI07_RS08030at 40.915 kb on - strand, within RPSI07_RS08030at 40.915 kb on - strand, within RPSI07_RS08030at 40.915 kb on - strand, within RPSI07_RS08030at 41.073 kb on + strand, within RPSI07_RS08030at 41.073 kb on + strand, within RPSI07_RS08030at 41.073 kb on + strand, within RPSI07_RS08030at 41.074 kb on - strand, within RPSI07_RS08030at 41.129 kb on + strand, within RPSI07_RS08030at 41.221 kb on - strand, within RPSI07_RS08030at 41.355 kb on + strand, within RPSI07_RS08030at 41.355 kb on + strand, within RPSI07_RS08030at 41.355 kb on + strand, within RPSI07_RS08030at 41.583 kb on + strandat 41.584 kb on - strandat 41.584 kb on - strandat 41.584 kb on - strandat 41.584 kb on - strandat 41.584 kb on - strandat 41.607 kb on + strandat 41.608 kb on - strandat 41.608 kb on - strandat 41.608 kb on - strandat 41.800 kb on + strand, within RPSI07_RS08035at 41.800 kb on + strand, within RPSI07_RS08035at 41.941 kb on + strand, within RPSI07_RS08035at 42.095 kb on + strand, within RPSI07_RS08035at 42.095 kb on + strandat 42.096 kb on - strand, within RPSI07_RS08035at 42.096 kb on - strand, within RPSI07_RS08035at 42.124 kb on + strand, within RPSI07_RS08035at 42.125 kb on - strand, within RPSI07_RS08035at 42.125 kb on - strand, within RPSI07_RS08035at 42.190 kb on + strand, within RPSI07_RS08035at 42.190 kb on + strand, within RPSI07_RS08035at 42.190 kb on - strand, within RPSI07_RS08035at 42.191 kb on - strand, within RPSI07_RS08035at 42.247 kb on + strand, within RPSI07_RS08035at 42.248 kb on - strand, within RPSI07_RS08035at 42.248 kb on - strand, within RPSI07_RS08035at 42.386 kb on + strandat 42.386 kb on + strandat 42.386 kb on + strandat 42.387 kb on - strandat 42.387 kb on - strandat 42.474 kb on + strandat 42.474 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol_and_CrystalViolet
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39,358 + RPSI07_RS08020 0.68 +1.0
39,358 + RPSI07_RS08020 0.68 -0.9
39,470 + RPSI07_RS08020 0.85 +0.2
39,470 + RPSI07_RS08020 0.85 -0.4
39,470 + RPSI07_RS08020 0.85 +0.1
39,471 - RPSI07_RS08020 0.85 +0.1
39,471 - RPSI07_RS08020 0.85 -1.9
39,471 - RPSI07_RS08020 0.85 -1.4
39,471 - RPSI07_RS08020 0.85 -1.9
39,471 - RPSI07_RS08020 0.85 +0.4
39,472 + RPSI07_RS08020 0.85 +0.9
39,472 + RPSI07_RS08020 0.85 +2.0
39,472 + RPSI07_RS08020 0.85 -0.7
39,473 - RPSI07_RS08020 0.85 -0.7
39,473 - RPSI07_RS08020 0.85 +1.0
39,473 - RPSI07_RS08020 0.85 +0.3
39,491 + RPSI07_RS08020 0.88 +2.0
39,491 + RPSI07_RS08020 0.88 +1.4
39,491 + RPSI07_RS08020 0.88 +2.9
39,724 + RPSI07_RS08025 0.20 +0.2
39,724 + RPSI07_RS08025 0.20 -1.0
39,724 + RPSI07_RS08025 0.20 -1.4
39,725 - RPSI07_RS08025 0.21 -0.3
39,877 + RPSI07_RS08025 0.41 -0.8
39,877 + RPSI07_RS08025 0.41 -0.1
39,877 + RPSI07_RS08025 0.41 -0.4
39,878 - RPSI07_RS08025 0.41 +1.4
40,325 + +0.1
40,325 + -0.1
40,326 - +0.1
40,467 + RPSI07_RS08030 0.11 -0.4
40,745 + RPSI07_RS08030 0.32 +2.3
40,746 + RPSI07_RS08030 0.32 -1.1
40,746 + RPSI07_RS08030 0.32 +1.8
40,747 - RPSI07_RS08030 0.32 -0.1
40,747 - RPSI07_RS08030 0.32 +1.4
40,747 - RPSI07_RS08030 0.32 -0.6
40,914 + RPSI07_RS08030 0.45 -0.5
40,915 - RPSI07_RS08030 0.45 +0.1
40,915 - RPSI07_RS08030 0.45 -1.9
40,915 - RPSI07_RS08030 0.45 +0.9
41,073 + RPSI07_RS08030 0.57 +2.0
41,073 + RPSI07_RS08030 0.57 -0.6
41,073 + RPSI07_RS08030 0.57 -0.4
41,074 - RPSI07_RS08030 0.57 -1.3
41,129 + RPSI07_RS08030 0.61 +0.3
41,221 - RPSI07_RS08030 0.68 -0.1
41,355 + RPSI07_RS08030 0.79 +0.0
41,355 + RPSI07_RS08030 0.79 +0.1
41,355 + RPSI07_RS08030 0.79 -0.7
41,583 + +0.2
41,584 - +1.3
41,584 - +0.5
41,584 - +2.2
41,584 - -1.6
41,584 - +0.7
41,607 + -2.0
41,608 - -0.1
41,608 - +2.2
41,608 - -0.3
41,800 + RPSI07_RS08035 0.21 +2.0
41,800 + RPSI07_RS08035 0.21 +0.5
41,941 + RPSI07_RS08035 0.39 +1.6
42,095 + RPSI07_RS08035 0.58 -0.1
42,095 + +3.0
42,096 - RPSI07_RS08035 0.58 -0.1
42,096 - RPSI07_RS08035 0.58 -0.4
42,124 + RPSI07_RS08035 0.62 +1.2
42,125 - RPSI07_RS08035 0.62 +0.3
42,125 - RPSI07_RS08035 0.62 +1.8
42,190 + RPSI07_RS08035 0.71 +1.4
42,190 + RPSI07_RS08035 0.71 -0.3
42,190 - RPSI07_RS08035 0.71 +0.4
42,191 - RPSI07_RS08035 0.71 +1.3
42,247 + RPSI07_RS08035 0.78 -1.4
42,248 - RPSI07_RS08035 0.78 +1.3
42,248 - RPSI07_RS08035 0.78 +0.5
42,386 + -2.2
42,386 + -1.1
42,386 + -0.9
42,387 - -0.7
42,387 - -0.9
42,474 + -1.3
42,474 + -0.7

Or see this region's nucleotide sequence