Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol_and_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS07800 and RPSI07_RS07805 are separated by 45 nucleotides RPSI07_RS07805 and RPSI07_RS07810 are separated by 124 nucleotides
RPSI07_RS07800: RPSI07_RS07800 - patatin, at 2,063,878 to 2,064,741
_RS07800
RPSI07_RS07805: RPSI07_RS07805 - alpha/beta hydrolase, at 2,064,787 to 2,065,680
_RS07805
RPSI07_RS07810: RPSI07_RS07810 - peptide chain release factor 3, at 2,065,805 to 2,067,400
_RS07810
Position (kb)
2064
2065
2066 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2064.979 kb on + strand, within RPSI07_RS07805 at 2064.979 kb on + strand, within RPSI07_RS07805 at 2065.135 kb on + strand, within RPSI07_RS07805 at 2065.205 kb on - strand, within RPSI07_RS07805 at 2065.291 kb on + strand, within RPSI07_RS07805 at 2065.291 kb on + strand, within RPSI07_RS07805 at 2065.291 kb on + strand, within RPSI07_RS07805 at 2065.292 kb on - strand, within RPSI07_RS07805 at 2065.292 kb on - strand, within RPSI07_RS07805 at 2065.292 kb on - strand, within RPSI07_RS07805 at 2065.292 kb on - strand, within RPSI07_RS07805 at 2065.386 kb on - strand, within RPSI07_RS07805 at 2065.386 kb on - strand, within RPSI07_RS07805 at 2065.386 kb on - strand, within RPSI07_RS07805 at 2065.393 kb on - strand, within RPSI07_RS07805 at 2065.807 kb on - strand at 2065.807 kb on - strand at 2065.890 kb on + strand at 2065.890 kb on + strand at 2065.890 kb on + strand at 2065.890 kb on + strand at 2065.891 kb on - strand at 2065.891 kb on - strand at 2065.891 kb on - strand at 2065.896 kb on + strand at 2065.896 kb on + strand at 2065.896 kb on + strand at 2065.896 kb on + strand at 2065.896 kb on + strand at 2065.897 kb on - strand at 2066.026 kb on - strand, within RPSI07_RS07810 at 2066.205 kb on + strand, within RPSI07_RS07810 at 2066.205 kb on + strand, within RPSI07_RS07810 at 2066.206 kb on - strand, within RPSI07_RS07810 at 2066.316 kb on + strand, within RPSI07_RS07810 at 2066.317 kb on - strand, within RPSI07_RS07810 at 2066.600 kb on + strand, within RPSI07_RS07810 at 2066.600 kb on + strand, within RPSI07_RS07810 at 2066.632 kb on - strand, within RPSI07_RS07810 at 2066.632 kb on - strand, within RPSI07_RS07810 at 2066.632 kb on - strand, within RPSI07_RS07810
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol_and_CrystalViolet remove 2,064,979 + RPSI07_RS07805 0.21 -0.9 2,064,979 + RPSI07_RS07805 0.21 -1.7 2,065,135 + RPSI07_RS07805 0.39 -1.7 2,065,205 - RPSI07_RS07805 0.47 -1.7 2,065,291 + RPSI07_RS07805 0.56 -1.3 2,065,291 + RPSI07_RS07805 0.56 -1.5 2,065,291 + RPSI07_RS07805 0.56 -1.1 2,065,292 - RPSI07_RS07805 0.56 -1.8 2,065,292 - RPSI07_RS07805 0.56 -0.7 2,065,292 - RPSI07_RS07805 0.56 -2.8 2,065,292 - RPSI07_RS07805 0.56 -1.5 2,065,386 - RPSI07_RS07805 0.67 -1.8 2,065,386 - RPSI07_RS07805 0.67 -0.0 2,065,386 - RPSI07_RS07805 0.67 -0.1 2,065,393 - RPSI07_RS07805 0.68 -0.5 2,065,807 - +2.4 2,065,807 - +0.5 2,065,890 + +1.3 2,065,890 + -1.5 2,065,890 + +1.7 2,065,890 + -0.6 2,065,891 - +2.6 2,065,891 - +0.5 2,065,891 - -1.5 2,065,896 + -1.1 2,065,896 + +0.3 2,065,896 + -0.1 2,065,896 + -1.4 2,065,896 + +0.5 2,065,897 - -0.3 2,066,026 - RPSI07_RS07810 0.14 -0.7 2,066,205 + RPSI07_RS07810 0.25 -0.2 2,066,205 + RPSI07_RS07810 0.25 +0.3 2,066,206 - RPSI07_RS07810 0.25 +0.4 2,066,316 + RPSI07_RS07810 0.32 -0.5 2,066,317 - RPSI07_RS07810 0.32 +0.7 2,066,600 + RPSI07_RS07810 0.50 -0.1 2,066,600 + RPSI07_RS07810 0.50 -0.1 2,066,632 - RPSI07_RS07810 0.52 -1.0 2,066,632 - RPSI07_RS07810 0.52 -0.3 2,066,632 - RPSI07_RS07810 0.52 -0.9
Or see this region's nucleotide sequence