Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol_and_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS03535 and RPSI07_RS03540 are separated by 92 nucleotides RPSI07_RS03540 and RPSI07_RS03545 are separated by 473 nucleotides
RPSI07_RS03535: RPSI07_RS03535 - methyl-accepting chemotaxis protein, at 960,046 to 961,602
_RS03535
RPSI07_RS03540: RPSI07_RS03540 - hypothetical protein, at 961,695 to 962,348
_RS03540
RPSI07_RS03545: RPSI07_RS03545 - leucine-rich repeat domain-containing protein, at 962,822 to 964,768
_RS03545
Position (kb) 961
962
963 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 961.103 kb on + strand, within RPSI07_RS03535 at 961.134 kb on - strand, within RPSI07_RS03535 at 961.134 kb on - strand, within RPSI07_RS03535 at 961.236 kb on - strand, within RPSI07_RS03535 at 961.675 kb on + strand at 961.675 kb on + strand at 961.676 kb on - strand at 961.676 kb on - strand at 961.676 kb on - strand at 961.676 kb on - strand at 961.676 kb on - strand at 961.892 kb on + strand, within RPSI07_RS03540 at 961.893 kb on - strand, within RPSI07_RS03540 at 961.893 kb on - strand, within RPSI07_RS03540 at 961.893 kb on - strand, within RPSI07_RS03540 at 962.083 kb on + strand, within RPSI07_RS03540 at 962.083 kb on + strand, within RPSI07_RS03540 at 962.083 kb on + strand, within RPSI07_RS03540 at 962.083 kb on + strand, within RPSI07_RS03540 at 962.084 kb on - strand, within RPSI07_RS03540 at 962.084 kb on - strand, within RPSI07_RS03540 at 962.084 kb on - strand, within RPSI07_RS03540 at 962.084 kb on - strand, within RPSI07_RS03540 at 962.084 kb on - strand, within RPSI07_RS03540 at 962.237 kb on - strand, within RPSI07_RS03540 at 962.275 kb on + strand, within RPSI07_RS03540 at 962.382 kb on - strand at 962.382 kb on - strand at 962.587 kb on + strand at 962.587 kb on + strand at 962.587 kb on + strand at 962.707 kb on + strand at 962.708 kb on - strand at 962.731 kb on + strand at 962.731 kb on + strand at 962.731 kb on + strand at 962.732 kb on - strand at 962.732 kb on - strand at 962.732 kb on - strand at 962.802 kb on - strand at 962.802 kb on - strand at 962.807 kb on + strand at 962.808 kb on - strand at 963.159 kb on + strand, within RPSI07_RS03545 at 963.159 kb on + strand, within RPSI07_RS03545 at 963.160 kb on - strand, within RPSI07_RS03545 at 963.160 kb on - strand, within RPSI07_RS03545 at 963.246 kb on - strand, within RPSI07_RS03545 at 963.246 kb on - strand, within RPSI07_RS03545
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol_and_CrystalViolet remove 961,103 + RPSI07_RS03535 0.68 -1.9 961,134 - RPSI07_RS03535 0.70 -3.2 961,134 - RPSI07_RS03535 0.70 +0.1 961,236 - RPSI07_RS03535 0.76 -1.9 961,675 + +0.6 961,675 + -1.5 961,676 - -0.8 961,676 - +1.1 961,676 - -1.4 961,676 - -0.7 961,676 - +1.3 961,892 + RPSI07_RS03540 0.30 +0.9 961,893 - RPSI07_RS03540 0.30 +0.5 961,893 - RPSI07_RS03540 0.30 -0.3 961,893 - RPSI07_RS03540 0.30 +1.8 962,083 + RPSI07_RS03540 0.59 +2.5 962,083 + RPSI07_RS03540 0.59 +1.1 962,083 + RPSI07_RS03540 0.59 +0.2 962,083 + RPSI07_RS03540 0.59 -0.4 962,084 - RPSI07_RS03540 0.59 -1.6 962,084 - RPSI07_RS03540 0.59 -0.1 962,084 - RPSI07_RS03540 0.59 -2.9 962,084 - RPSI07_RS03540 0.59 +0.1 962,084 - RPSI07_RS03540 0.59 -1.0 962,237 - RPSI07_RS03540 0.83 +0.3 962,275 + RPSI07_RS03540 0.89 -0.3 962,382 - +1.2 962,382 - +3.1 962,587 + +0.5 962,587 + +0.9 962,587 + +0.0 962,707 + +1.1 962,708 - +0.5 962,731 + +0.1 962,731 + -2.5 962,731 + -1.7 962,732 - -0.2 962,732 - -1.4 962,732 - +0.7 962,802 - -2.0 962,802 - +0.8 962,807 + +1.6 962,808 - +1.1 963,159 + RPSI07_RS03545 0.17 -0.2 963,159 + RPSI07_RS03545 0.17 -0.8 963,160 - RPSI07_RS03545 0.17 +0.4 963,160 - RPSI07_RS03545 0.17 +0.9 963,246 - RPSI07_RS03545 0.22 +0.0 963,246 - RPSI07_RS03545 0.22 +0.4
Or see this region's nucleotide sequence