Strain Fitness in Ralstonia solanacearum PSI07 around RPSI07_RS21760

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRPSI07_RS21755 and RPSI07_RS21760 overlap by 4 nucleotidesRPSI07_RS21760 and RPSI07_RS21765 are separated by 123 nucleotides RPSI07_RS21755: RPSI07_RS21755 - ubiquinone biosynthesis regulatory protein kinase UbiB, at 3,041,156 to 3,042,733 _RS21755 RPSI07_RS21760: RPSI07_RS21760 - SCP2 domain-containing protein, at 3,042,730 to 3,043,389 _RS21760 RPSI07_RS21765: RPSI07_RS21765 - membrane protein, at 3,043,513 to 3,044,550 _RS21765 Position (kb) 3042 3043 3044Strain fitness (log2 ratio) -1 0 1at 3043.655 kb on + strand, within RPSI07_RS21765at 3043.656 kb on - strand, within RPSI07_RS21765at 3043.838 kb on + strand, within RPSI07_RS21765at 3043.838 kb on + strand, within RPSI07_RS21765at 3043.838 kb on + strand, within RPSI07_RS21765at 3043.839 kb on - strand, within RPSI07_RS21765at 3044.085 kb on - strand, within RPSI07_RS21765at 3044.085 kb on - strand, within RPSI07_RS21765at 3044.126 kb on + strand, within RPSI07_RS21765at 3044.262 kb on - strand, within RPSI07_RS21765

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet
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3,043,655 + RPSI07_RS21765 0.14 +1.5
3,043,656 - RPSI07_RS21765 0.14 -0.3
3,043,838 + RPSI07_RS21765 0.31 +0.3
3,043,838 + RPSI07_RS21765 0.31 +0.4
3,043,838 + RPSI07_RS21765 0.31 +1.1
3,043,839 - RPSI07_RS21765 0.31 -1.1
3,044,085 - RPSI07_RS21765 0.55 -0.7
3,044,085 - RPSI07_RS21765 0.55 +0.6
3,044,126 + RPSI07_RS21765 0.59 +1.2
3,044,262 - RPSI07_RS21765 0.72 +0.2

Or see this region's nucleotide sequence