Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS21305 and RPSI07_RS21310 are separated by 443 nucleotides RPSI07_RS21310 and RPSI07_RS21315 are separated by 153 nucleotides
RPSI07_RS21305: RPSI07_RS21305 - DNA-binding response regulator, at 2,941,423 to 2,942,163
_RS21305
RPSI07_RS21310: RPSI07_RS21310 - LysR family transcriptional regulator, at 2,942,607 to 2,943,524
_RS21310
RPSI07_RS21315: RPSI07_RS21315 - pirin family protein, at 2,943,678 to 2,944,574
_RS21315
Position (kb)
2942
2943
2944 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5
6
7 at 2941.673 kb on - strand, within RPSI07_RS21305 at 2941.673 kb on - strand, within RPSI07_RS21305 at 2941.673 kb on - strand, within RPSI07_RS21305 at 2941.791 kb on - strand, within RPSI07_RS21305 at 2941.868 kb on + strand, within RPSI07_RS21305 at 2941.869 kb on - strand, within RPSI07_RS21305 at 2941.869 kb on - strand, within RPSI07_RS21305 at 2942.030 kb on + strand, within RPSI07_RS21305 at 2942.031 kb on - strand, within RPSI07_RS21305 at 2942.157 kb on + strand at 2942.800 kb on + strand, within RPSI07_RS21310 at 2942.801 kb on - strand, within RPSI07_RS21310 at 2942.801 kb on - strand, within RPSI07_RS21310 at 2942.851 kb on + strand, within RPSI07_RS21310 at 2942.852 kb on - strand, within RPSI07_RS21310 at 2942.852 kb on - strand, within RPSI07_RS21310 at 2943.016 kb on + strand, within RPSI07_RS21310 at 2943.016 kb on + strand, within RPSI07_RS21310 at 2943.016 kb on + strand, within RPSI07_RS21310 at 2943.017 kb on - strand, within RPSI07_RS21310 at 2943.176 kb on - strand, within RPSI07_RS21310 at 2943.332 kb on - strand, within RPSI07_RS21310 at 2943.381 kb on + strand, within RPSI07_RS21310 at 2943.539 kb on - strand at 2943.539 kb on - strand at 2943.567 kb on - strand at 2943.610 kb on + strand at 2943.610 kb on + strand at 2943.611 kb on - strand at 2943.633 kb on + strand at 2943.634 kb on - strand at 2943.634 kb on - strand at 2943.677 kb on + strand at 2943.677 kb on + strand at 2943.691 kb on + strand at 2943.692 kb on - strand at 2943.692 kb on - strand at 2943.858 kb on + strand, within RPSI07_RS21315 at 2943.858 kb on + strand, within RPSI07_RS21315 at 2943.859 kb on - strand, within RPSI07_RS21315 at 2943.859 kb on - strand, within RPSI07_RS21315 at 2943.909 kb on + strand, within RPSI07_RS21315 at 2943.909 kb on + strand, within RPSI07_RS21315 at 2943.909 kb on + strand at 2943.909 kb on + strand, within RPSI07_RS21315 at 2943.910 kb on - strand, within RPSI07_RS21315 at 2943.910 kb on - strand at 2943.910 kb on - strand, within RPSI07_RS21315 at 2943.910 kb on - strand, within RPSI07_RS21315 at 2943.910 kb on - strand, within RPSI07_RS21315 at 2943.910 kb on - strand, within RPSI07_RS21315 at 2943.910 kb on - strand, within RPSI07_RS21315 at 2944.302 kb on + strand, within RPSI07_RS21315 at 2944.302 kb on + strand, within RPSI07_RS21315 at 2944.303 kb on - strand, within RPSI07_RS21315 at 2944.497 kb on + strand at 2944.497 kb on + strand at 2944.497 kb on + strand at 2944.498 kb on - strand at 2944.498 kb on - strand at 2944.498 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet remove 2,941,673 - RPSI07_RS21305 0.34 -1.0 2,941,673 - RPSI07_RS21305 0.34 +1.2 2,941,673 - RPSI07_RS21305 0.34 +5.3 2,941,791 - RPSI07_RS21305 0.50 -1.0 2,941,868 + RPSI07_RS21305 0.60 +1.2 2,941,869 - RPSI07_RS21305 0.60 -2.6 2,941,869 - RPSI07_RS21305 0.60 -0.2 2,942,030 + RPSI07_RS21305 0.82 +0.5 2,942,031 - RPSI07_RS21305 0.82 +1.0 2,942,157 + -1.3 2,942,800 + RPSI07_RS21310 0.21 +0.5 2,942,801 - RPSI07_RS21310 0.21 -0.3 2,942,801 - RPSI07_RS21310 0.21 -0.5 2,942,851 + RPSI07_RS21310 0.27 -1.3 2,942,852 - RPSI07_RS21310 0.27 -1.1 2,942,852 - RPSI07_RS21310 0.27 +0.8 2,943,016 + RPSI07_RS21310 0.45 -0.2 2,943,016 + RPSI07_RS21310 0.45 -0.6 2,943,016 + RPSI07_RS21310 0.45 +1.5 2,943,017 - RPSI07_RS21310 0.45 +0.9 2,943,176 - RPSI07_RS21310 0.62 -1.6 2,943,332 - RPSI07_RS21310 0.79 +2.5 2,943,381 + RPSI07_RS21310 0.84 +0.7 2,943,539 - +1.5 2,943,539 - -1.3 2,943,567 - -0.4 2,943,610 + -0.7 2,943,610 + +1.8 2,943,611 - +1.1 2,943,633 + -1.2 2,943,634 - +3.5 2,943,634 - -0.1 2,943,677 + -0.3 2,943,677 + +0.2 2,943,691 + -0.4 2,943,692 - -2.4 2,943,692 - -1.1 2,943,858 + RPSI07_RS21315 0.20 -0.1 2,943,858 + RPSI07_RS21315 0.20 -1.4 2,943,859 - RPSI07_RS21315 0.20 -1.6 2,943,859 - RPSI07_RS21315 0.20 -0.1 2,943,909 + RPSI07_RS21315 0.26 -0.4 2,943,909 + RPSI07_RS21315 0.26 +7.5 2,943,909 + +1.5 2,943,909 + RPSI07_RS21315 0.26 +0.3 2,943,910 - RPSI07_RS21315 0.26 -1.4 2,943,910 - +0.5 2,943,910 - RPSI07_RS21315 0.26 -0.2 2,943,910 - RPSI07_RS21315 0.26 -0.7 2,943,910 - RPSI07_RS21315 0.26 -1.2 2,943,910 - RPSI07_RS21315 0.26 -0.1 2,943,910 - RPSI07_RS21315 0.26 -0.6 2,944,302 + RPSI07_RS21315 0.70 +0.5 2,944,302 + RPSI07_RS21315 0.70 -1.9 2,944,303 - RPSI07_RS21315 0.70 +0.1 2,944,497 + +0.7 2,944,497 + -0.5 2,944,497 + -0.2 2,944,498 - -0.6 2,944,498 - -0.5 2,944,498 - -1.0
Or see this region's nucleotide sequence