Strain Fitness in Ralstonia solanacearum PSI07 around RPSI07_RS21310

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRPSI07_RS21305 and RPSI07_RS21310 are separated by 443 nucleotidesRPSI07_RS21310 and RPSI07_RS21315 are separated by 153 nucleotides RPSI07_RS21305: RPSI07_RS21305 - DNA-binding response regulator, at 2,941,423 to 2,942,163 _RS21305 RPSI07_RS21310: RPSI07_RS21310 - LysR family transcriptional regulator, at 2,942,607 to 2,943,524 _RS21310 RPSI07_RS21315: RPSI07_RS21315 - pirin family protein, at 2,943,678 to 2,944,574 _RS21315 Position (kb) 2942 2943 2944Strain fitness (log2 ratio) -2 -1 0 1 2 3 4 5 6 7at 2941.673 kb on - strand, within RPSI07_RS21305at 2941.673 kb on - strand, within RPSI07_RS21305at 2941.673 kb on - strand, within RPSI07_RS21305at 2941.791 kb on - strand, within RPSI07_RS21305at 2941.868 kb on + strand, within RPSI07_RS21305at 2941.869 kb on - strand, within RPSI07_RS21305at 2941.869 kb on - strand, within RPSI07_RS21305at 2942.030 kb on + strand, within RPSI07_RS21305at 2942.031 kb on - strand, within RPSI07_RS21305at 2942.157 kb on + strandat 2942.800 kb on + strand, within RPSI07_RS21310at 2942.801 kb on - strand, within RPSI07_RS21310at 2942.801 kb on - strand, within RPSI07_RS21310at 2942.851 kb on + strand, within RPSI07_RS21310at 2942.852 kb on - strand, within RPSI07_RS21310at 2942.852 kb on - strand, within RPSI07_RS21310at 2943.016 kb on + strand, within RPSI07_RS21310at 2943.016 kb on + strand, within RPSI07_RS21310at 2943.016 kb on + strand, within RPSI07_RS21310at 2943.017 kb on - strand, within RPSI07_RS21310at 2943.176 kb on - strand, within RPSI07_RS21310at 2943.332 kb on - strand, within RPSI07_RS21310at 2943.381 kb on + strand, within RPSI07_RS21310at 2943.539 kb on - strandat 2943.539 kb on - strandat 2943.567 kb on - strandat 2943.610 kb on + strandat 2943.610 kb on + strandat 2943.611 kb on - strandat 2943.633 kb on + strandat 2943.634 kb on - strandat 2943.634 kb on - strandat 2943.677 kb on + strandat 2943.677 kb on + strandat 2943.691 kb on + strandat 2943.692 kb on - strandat 2943.692 kb on - strandat 2943.858 kb on + strand, within RPSI07_RS21315at 2943.858 kb on + strand, within RPSI07_RS21315at 2943.859 kb on - strand, within RPSI07_RS21315at 2943.859 kb on - strand, within RPSI07_RS21315at 2943.909 kb on + strand, within RPSI07_RS21315at 2943.909 kb on + strand, within RPSI07_RS21315at 2943.909 kb on + strandat 2943.909 kb on + strand, within RPSI07_RS21315at 2943.910 kb on - strand, within RPSI07_RS21315at 2943.910 kb on - strandat 2943.910 kb on - strand, within RPSI07_RS21315at 2943.910 kb on - strand, within RPSI07_RS21315at 2943.910 kb on - strand, within RPSI07_RS21315at 2943.910 kb on - strand, within RPSI07_RS21315at 2943.910 kb on - strand, within RPSI07_RS21315at 2944.302 kb on + strand, within RPSI07_RS21315at 2944.302 kb on + strand, within RPSI07_RS21315at 2944.303 kb on - strand, within RPSI07_RS21315at 2944.497 kb on + strandat 2944.497 kb on + strandat 2944.497 kb on + strandat 2944.498 kb on - strandat 2944.498 kb on - strandat 2944.498 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet
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2,941,673 - RPSI07_RS21305 0.34 -1.0
2,941,673 - RPSI07_RS21305 0.34 +1.2
2,941,673 - RPSI07_RS21305 0.34 +5.3
2,941,791 - RPSI07_RS21305 0.50 -1.0
2,941,868 + RPSI07_RS21305 0.60 +1.2
2,941,869 - RPSI07_RS21305 0.60 -2.6
2,941,869 - RPSI07_RS21305 0.60 -0.2
2,942,030 + RPSI07_RS21305 0.82 +0.5
2,942,031 - RPSI07_RS21305 0.82 +1.0
2,942,157 + -1.3
2,942,800 + RPSI07_RS21310 0.21 +0.5
2,942,801 - RPSI07_RS21310 0.21 -0.3
2,942,801 - RPSI07_RS21310 0.21 -0.5
2,942,851 + RPSI07_RS21310 0.27 -1.3
2,942,852 - RPSI07_RS21310 0.27 -1.1
2,942,852 - RPSI07_RS21310 0.27 +0.8
2,943,016 + RPSI07_RS21310 0.45 -0.2
2,943,016 + RPSI07_RS21310 0.45 -0.6
2,943,016 + RPSI07_RS21310 0.45 +1.5
2,943,017 - RPSI07_RS21310 0.45 +0.9
2,943,176 - RPSI07_RS21310 0.62 -1.6
2,943,332 - RPSI07_RS21310 0.79 +2.5
2,943,381 + RPSI07_RS21310 0.84 +0.7
2,943,539 - +1.5
2,943,539 - -1.3
2,943,567 - -0.4
2,943,610 + -0.7
2,943,610 + +1.8
2,943,611 - +1.1
2,943,633 + -1.2
2,943,634 - +3.5
2,943,634 - -0.1
2,943,677 + -0.3
2,943,677 + +0.2
2,943,691 + -0.4
2,943,692 - -2.4
2,943,692 - -1.1
2,943,858 + RPSI07_RS21315 0.20 -0.1
2,943,858 + RPSI07_RS21315 0.20 -1.4
2,943,859 - RPSI07_RS21315 0.20 -1.6
2,943,859 - RPSI07_RS21315 0.20 -0.1
2,943,909 + RPSI07_RS21315 0.26 -0.4
2,943,909 + RPSI07_RS21315 0.26 +7.5
2,943,909 + +1.5
2,943,909 + RPSI07_RS21315 0.26 +0.3
2,943,910 - RPSI07_RS21315 0.26 -1.4
2,943,910 - +0.5
2,943,910 - RPSI07_RS21315 0.26 -0.2
2,943,910 - RPSI07_RS21315 0.26 -0.7
2,943,910 - RPSI07_RS21315 0.26 -1.2
2,943,910 - RPSI07_RS21315 0.26 -0.1
2,943,910 - RPSI07_RS21315 0.26 -0.6
2,944,302 + RPSI07_RS21315 0.70 +0.5
2,944,302 + RPSI07_RS21315 0.70 -1.9
2,944,303 - RPSI07_RS21315 0.70 +0.1
2,944,497 + +0.7
2,944,497 + -0.5
2,944,497 + -0.2
2,944,498 - -0.6
2,944,498 - -0.5
2,944,498 - -1.0

Or see this region's nucleotide sequence