Strain Fitness in Ralstonia solanacearum PSI07 around RPSI07_RS21295

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRPSI07_RS21280 and RPSI07_RS21285 are separated by 50 nucleotidesRPSI07_RS21285 and RPSI07_RS21290 are separated by 118 nucleotidesRPSI07_RS21290 and RPSI07_RS21295 are separated by 441 nucleotidesRPSI07_RS21295 and RPSI07_RS21300 are separated by 21 nucleotidesRPSI07_RS21300 and RPSI07_RS21305 are separated by 68 nucleotides RPSI07_RS21280: RPSI07_RS21280 - MFS transporter, at 2,936,973 to 2,938,676 _RS21280 RPSI07_RS21285: RPSI07_RS21285 - tRNA-Met, at 2,938,727 to 2,938,803 _RS21285 RPSI07_RS21290: RPSI07_RS21290 - hypothetical protein, at 2,938,922 to 2,939,134 _RS21290 RPSI07_RS21295: RPSI07_RS21295 - FAD-binding monooxygenase, at 2,939,576 to 2,940,772 _RS21295 RPSI07_RS21300: RPSI07_RS21300 - hypothetical protein, at 2,940,794 to 2,941,354 _RS21300 RPSI07_RS21305: RPSI07_RS21305 - DNA-binding response regulator, at 2,941,423 to 2,942,163 _RS21305 Position (kb) 2939 2940 2941Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4 5at 2938.662 kb on + strandat 2938.662 kb on + strandat 2938.663 kb on - strandat 2938.663 kb on - strandat 2938.663 kb on - strandat 2938.663 kb on - strandat 2938.986 kb on + strand, within RPSI07_RS21290at 2939.134 kb on + strandat 2939.343 kb on + strandat 2939.344 kb on - strandat 2939.494 kb on + strandat 2939.495 kb on - strandat 2939.495 kb on - strandat 2939.495 kb on - strandat 2939.545 kb on + strandat 2939.545 kb on + strandat 2939.970 kb on - strand, within RPSI07_RS21295at 2940.092 kb on + strand, within RPSI07_RS21295at 2940.416 kb on + strand, within RPSI07_RS21295at 2940.417 kb on - strand, within RPSI07_RS21295at 2940.581 kb on + strand, within RPSI07_RS21295at 2940.582 kb on - strand, within RPSI07_RS21295at 2941.274 kb on + strand, within RPSI07_RS21300at 2941.275 kb on - strand, within RPSI07_RS21300at 2941.352 kb on + strandat 2941.352 kb on + strandat 2941.353 kb on - strandat 2941.353 kb on - strandat 2941.425 kb on - strandat 2941.500 kb on - strand, within RPSI07_RS21305at 2941.511 kb on + strand, within RPSI07_RS21305at 2941.511 kb on + strand, within RPSI07_RS21305at 2941.512 kb on - strand, within RPSI07_RS21305at 2941.512 kb on - strand, within RPSI07_RS21305at 2941.512 kb on - strand, within RPSI07_RS21305at 2941.673 kb on - strand, within RPSI07_RS21305at 2941.673 kb on - strand, within RPSI07_RS21305at 2941.673 kb on - strand, within RPSI07_RS21305

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet
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2,938,662 + +0.4
2,938,662 + -0.7
2,938,663 - -0.7
2,938,663 - -0.4
2,938,663 - -0.3
2,938,663 - -0.8
2,938,986 + RPSI07_RS21290 0.30 +0.8
2,939,134 + -2.9
2,939,343 + -2.2
2,939,344 - +0.2
2,939,494 + -0.4
2,939,495 - -1.3
2,939,495 - +0.6
2,939,495 - +0.6
2,939,545 + +0.6
2,939,545 + +0.2
2,939,970 - RPSI07_RS21295 0.33 -1.1
2,940,092 + RPSI07_RS21295 0.43 +0.8
2,940,416 + RPSI07_RS21295 0.70 +1.5
2,940,417 - RPSI07_RS21295 0.70 -0.4
2,940,581 + RPSI07_RS21295 0.84 +0.2
2,940,582 - RPSI07_RS21295 0.84 -0.1
2,941,274 + RPSI07_RS21300 0.86 +0.2
2,941,275 - RPSI07_RS21300 0.86 +0.8
2,941,352 + -1.4
2,941,352 + -0.3
2,941,353 - -1.2
2,941,353 - +1.5
2,941,425 - +0.2
2,941,500 - RPSI07_RS21305 0.10 -0.4
2,941,511 + RPSI07_RS21305 0.12 -0.8
2,941,511 + RPSI07_RS21305 0.12 +0.3
2,941,512 - RPSI07_RS21305 0.12 -0.7
2,941,512 - RPSI07_RS21305 0.12 +0.1
2,941,512 - RPSI07_RS21305 0.12 +0.4
2,941,673 - RPSI07_RS21305 0.34 -1.0
2,941,673 - RPSI07_RS21305 0.34 +1.2
2,941,673 - RPSI07_RS21305 0.34 +5.3

Or see this region's nucleotide sequence