Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS21205 and RPSI07_RS21210 are separated by 94 nucleotides RPSI07_RS21210 and RPSI07_RS25230 are separated by 162 nucleotides RPSI07_RS25230 and RPSI07_RS21215 are separated by 51 nucleotides
RPSI07_RS21205: RPSI07_RS21205 - peptidase M48, at 2,921,519 to 2,923,207
_RS21205
RPSI07_RS21210: RPSI07_RS21210 - cyclic pyranopterin monophosphate synthase MoaC, at 2,923,302 to 2,923,778
_RS21210
RPSI07_RS25230: RPSI07_RS25230 - hypothetical protein, at 2,923,941 to 2,924,171
_RS25230
RPSI07_RS21215: RPSI07_RS21215 - polymerase, at 2,924,223 to 2,925,959
_RS21215
Position (kb)
2923
2924 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2922.391 kb on - strand, within RPSI07_RS21205 at 2922.391 kb on - strand, within RPSI07_RS21205 at 2922.700 kb on + strand, within RPSI07_RS21205 at 2922.700 kb on + strand, within RPSI07_RS21205 at 2922.700 kb on + strand, within RPSI07_RS21205 at 2922.702 kb on + strand, within RPSI07_RS21205 at 2922.703 kb on - strand, within RPSI07_RS21205 at 2922.776 kb on - strand, within RPSI07_RS21205 at 2922.837 kb on + strand, within RPSI07_RS21205 at 2922.837 kb on + strand, within RPSI07_RS21205 at 2922.864 kb on + strand, within RPSI07_RS21205 at 2922.970 kb on - strand, within RPSI07_RS21205 at 2922.970 kb on - strand, within RPSI07_RS21205 at 2923.031 kb on + strand, within RPSI07_RS21205 at 2923.267 kb on + strand at 2923.267 kb on + strand at 2923.268 kb on - strand at 2923.273 kb on - strand at 2923.584 kb on + strand, within RPSI07_RS21210 at 2923.585 kb on - strand, within RPSI07_RS21210 at 2923.680 kb on + strand, within RPSI07_RS21210 at 2923.680 kb on + strand, within RPSI07_RS21210 at 2923.680 kb on + strand, within RPSI07_RS21210 at 2923.680 kb on + strand, within RPSI07_RS21210 at 2923.681 kb on - strand, within RPSI07_RS21210 at 2923.681 kb on - strand, within RPSI07_RS21210 at 2923.726 kb on + strand, within RPSI07_RS21210 at 2923.726 kb on + strand, within RPSI07_RS21210 at 2923.726 kb on + strand, within RPSI07_RS21210 at 2923.726 kb on + strand, within RPSI07_RS21210 at 2923.726 kb on + strand, within RPSI07_RS21210 at 2923.726 kb on + strand, within RPSI07_RS21210 at 2923.726 kb on + strand, within RPSI07_RS21210 at 2923.726 kb on + strand, within RPSI07_RS21210 at 2923.727 kb on - strand, within RPSI07_RS21210 at 2923.727 kb on - strand, within RPSI07_RS21210 at 2923.727 kb on - strand, within RPSI07_RS21210 at 2923.727 kb on - strand, within RPSI07_RS21210 at 2923.727 kb on - strand, within RPSI07_RS21210 at 2923.776 kb on + strand at 2923.830 kb on - strand at 2923.879 kb on + strand at 2923.880 kb on + strand at 2923.929 kb on - strand at 2923.929 kb on - strand at 2923.929 kb on - strand at 2923.987 kb on + strand, within RPSI07_RS25230 at 2923.997 kb on + strand, within RPSI07_RS25230 at 2923.997 kb on + strand, within RPSI07_RS25230 at 2923.998 kb on - strand, within RPSI07_RS25230 at 2924.033 kb on + strand, within RPSI07_RS25230 at 2924.034 kb on - strand, within RPSI07_RS25230 at 2924.067 kb on + strand, within RPSI07_RS25230 at 2924.089 kb on + strand, within RPSI07_RS25230 at 2924.090 kb on - strand, within RPSI07_RS25230 at 2924.090 kb on - strand, within RPSI07_RS25230 at 2924.090 kb on - strand, within RPSI07_RS25230 at 2924.206 kb on + strand at 2924.207 kb on - strand at 2924.207 kb on - strand at 2924.207 kb on - strand at 2924.626 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet remove 2,922,391 - RPSI07_RS21205 0.52 -0.1 2,922,391 - RPSI07_RS21205 0.52 -0.7 2,922,700 + RPSI07_RS21205 0.70 -1.0 2,922,700 + RPSI07_RS21205 0.70 -0.9 2,922,700 + RPSI07_RS21205 0.70 +0.7 2,922,702 + RPSI07_RS21205 0.70 -0.9 2,922,703 - RPSI07_RS21205 0.70 -0.1 2,922,776 - RPSI07_RS21205 0.74 -2.1 2,922,837 + RPSI07_RS21205 0.78 -0.1 2,922,837 + RPSI07_RS21205 0.78 +0.5 2,922,864 + RPSI07_RS21205 0.80 +0.4 2,922,970 - RPSI07_RS21205 0.86 -1.4 2,922,970 - RPSI07_RS21205 0.86 +0.8 2,923,031 + RPSI07_RS21205 0.90 -0.4 2,923,267 + -1.6 2,923,267 + -0.7 2,923,268 - +0.9 2,923,273 - +2.4 2,923,584 + RPSI07_RS21210 0.59 -0.3 2,923,585 - RPSI07_RS21210 0.59 +0.2 2,923,680 + RPSI07_RS21210 0.79 -0.2 2,923,680 + RPSI07_RS21210 0.79 +0.3 2,923,680 + RPSI07_RS21210 0.79 -2.0 2,923,680 + RPSI07_RS21210 0.79 -2.0 2,923,681 - RPSI07_RS21210 0.79 +1.4 2,923,681 - RPSI07_RS21210 0.79 +1.2 2,923,726 + RPSI07_RS21210 0.89 -0.7 2,923,726 + RPSI07_RS21210 0.89 +1.4 2,923,726 + RPSI07_RS21210 0.89 +0.2 2,923,726 + RPSI07_RS21210 0.89 -1.9 2,923,726 + RPSI07_RS21210 0.89 -2.2 2,923,726 + RPSI07_RS21210 0.89 -0.1 2,923,726 + RPSI07_RS21210 0.89 +0.0 2,923,726 + RPSI07_RS21210 0.89 -0.9 2,923,727 - RPSI07_RS21210 0.89 -0.5 2,923,727 - RPSI07_RS21210 0.89 -0.1 2,923,727 - RPSI07_RS21210 0.89 -0.1 2,923,727 - RPSI07_RS21210 0.89 +0.8 2,923,727 - RPSI07_RS21210 0.89 -0.7 2,923,776 + -0.1 2,923,830 - -1.0 2,923,879 + -1.1 2,923,880 + -0.8 2,923,929 - -0.8 2,923,929 - +1.4 2,923,929 - +0.2 2,923,987 + RPSI07_RS25230 0.20 +0.4 2,923,997 + RPSI07_RS25230 0.24 -0.7 2,923,997 + RPSI07_RS25230 0.24 +1.4 2,923,998 - RPSI07_RS25230 0.25 -1.1 2,924,033 + RPSI07_RS25230 0.40 -2.2 2,924,034 - RPSI07_RS25230 0.40 -2.1 2,924,067 + RPSI07_RS25230 0.55 -1.1 2,924,089 + RPSI07_RS25230 0.64 +0.4 2,924,090 - RPSI07_RS25230 0.65 +0.1 2,924,090 - RPSI07_RS25230 0.65 +0.9 2,924,090 - RPSI07_RS25230 0.65 -0.4 2,924,206 + +0.9 2,924,207 - -0.1 2,924,207 - +0.2 2,924,207 - -0.6 2,924,626 + +0.4
Or see this region's nucleotide sequence