Strain Fitness in Ralstonia solanacearum PSI07 around RPSI07_RS20535

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRPSI07_RS25215 and RPSI07_RS20530 are separated by 5 nucleotidesRPSI07_RS20530 and RPSI07_RS20535 are separated by 86 nucleotidesRPSI07_RS20535 and RPSI07_RS20540 are separated by 27 nucleotidesRPSI07_RS20540 and RPSI07_RS20545 are separated by 69 nucleotides RPSI07_RS25215: RPSI07_RS25215 - hypothetical protein, at 2,780,163 to 2,780,384 _RS25215 RPSI07_RS20530: RPSI07_RS20530 - TetR/AcrR family transcriptional regulator, at 2,780,390 to 2,781,133 _RS20530 RPSI07_RS20535: RPSI07_RS20535 - protein-L-isoaspartate O-methyltransferase, at 2,781,220 to 2,781,870 _RS20535 RPSI07_RS20540: RPSI07_RS20540 - sulfurtransferase, at 2,781,898 to 2,782,230 _RS20540 RPSI07_RS20545: RPSI07_RS20545 - channel protein TolC, at 2,782,300 to 2,783,754 _RS20545 Position (kb) 2781 2782Strain fitness (log2 ratio) -2 -1 0 1 2 3at 2780.234 kb on - strand, within RPSI07_RS25215at 2780.234 kb on - strand, within RPSI07_RS25215at 2780.311 kb on - strand, within RPSI07_RS25215at 2780.316 kb on - strand, within RPSI07_RS25215at 2780.558 kb on + strand, within RPSI07_RS20530at 2780.558 kb on + strand, within RPSI07_RS20530at 2780.558 kb on + strand, within RPSI07_RS20530at 2780.559 kb on - strand, within RPSI07_RS20530at 2780.564 kb on + strand, within RPSI07_RS20530at 2780.564 kb on + strand, within RPSI07_RS20530at 2780.564 kb on + strand, within RPSI07_RS20530at 2780.564 kb on + strand, within RPSI07_RS20530at 2780.564 kb on + strandat 2780.565 kb on - strand, within RPSI07_RS20530at 2780.565 kb on - strand, within RPSI07_RS20530at 2780.565 kb on - strand, within RPSI07_RS20530at 2780.565 kb on - strand, within RPSI07_RS20530at 2780.786 kb on + strand, within RPSI07_RS20530at 2780.786 kb on + strand, within RPSI07_RS20530at 2780.786 kb on + strand, within RPSI07_RS20530at 2780.786 kb on + strand, within RPSI07_RS20530at 2780.789 kb on + strand, within RPSI07_RS20530at 2781.170 kb on + strandat 2781.328 kb on + strand, within RPSI07_RS20535at 2781.328 kb on + strand, within RPSI07_RS20535at 2781.328 kb on + strand, within RPSI07_RS20535at 2781.328 kb on + strand, within RPSI07_RS20535at 2781.328 kb on + strand, within RPSI07_RS20535at 2781.328 kb on + strand, within RPSI07_RS20535at 2781.329 kb on - strand, within RPSI07_RS20535at 2781.329 kb on - strand, within RPSI07_RS20535at 2781.487 kb on + strand, within RPSI07_RS20535at 2781.646 kb on + strand, within RPSI07_RS20535at 2781.820 kb on + strandat 2781.820 kb on + strandat 2781.820 kb on + strandat 2781.820 kb on + strandat 2781.820 kb on + strandat 2782.166 kb on - strand, within RPSI07_RS20540at 2782.225 kb on + strandat 2782.225 kb on + strandat 2782.299 kb on + strandat 2782.299 kb on + strandat 2782.299 kb on + strandat 2782.299 kb on + strandat 2782.300 kb on - strandat 2782.669 kb on + strand, within RPSI07_RS20545at 2782.669 kb on + strand, within RPSI07_RS20545at 2782.670 kb on - strand, within RPSI07_RS20545at 2782.756 kb on + strand, within RPSI07_RS20545at 2782.828 kb on + strand, within RPSI07_RS20545

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet
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2,780,234 - RPSI07_RS25215 0.32 -1.9
2,780,234 - RPSI07_RS25215 0.32 -0.0
2,780,311 - RPSI07_RS25215 0.67 -0.8
2,780,316 - RPSI07_RS25215 0.69 -0.1
2,780,558 + RPSI07_RS20530 0.23 -0.7
2,780,558 + RPSI07_RS20530 0.23 +0.7
2,780,558 + RPSI07_RS20530 0.23 -0.4
2,780,559 - RPSI07_RS20530 0.23 +0.1
2,780,564 + RPSI07_RS20530 0.23 -0.3
2,780,564 + RPSI07_RS20530 0.23 +1.0
2,780,564 + RPSI07_RS20530 0.23 +0.1
2,780,564 + RPSI07_RS20530 0.23 +1.1
2,780,564 + +3.1
2,780,565 - RPSI07_RS20530 0.24 -0.3
2,780,565 - RPSI07_RS20530 0.24 -0.8
2,780,565 - RPSI07_RS20530 0.24 +0.1
2,780,565 - RPSI07_RS20530 0.24 -0.8
2,780,786 + RPSI07_RS20530 0.53 +2.1
2,780,786 + RPSI07_RS20530 0.53 +0.9
2,780,786 + RPSI07_RS20530 0.53 +0.9
2,780,786 + RPSI07_RS20530 0.53 +1.5
2,780,789 + RPSI07_RS20530 0.54 +0.6
2,781,170 + -0.0
2,781,328 + RPSI07_RS20535 0.17 +0.3
2,781,328 + RPSI07_RS20535 0.17 -0.5
2,781,328 + RPSI07_RS20535 0.17 +0.5
2,781,328 + RPSI07_RS20535 0.17 -0.1
2,781,328 + RPSI07_RS20535 0.17 -1.0
2,781,328 + RPSI07_RS20535 0.17 -1.4
2,781,329 - RPSI07_RS20535 0.17 -1.4
2,781,329 - RPSI07_RS20535 0.17 -0.1
2,781,487 + RPSI07_RS20535 0.41 -0.9
2,781,646 + RPSI07_RS20535 0.65 -1.3
2,781,820 + -1.7
2,781,820 + -0.3
2,781,820 + -1.2
2,781,820 + +1.5
2,781,820 + -0.5
2,782,166 - RPSI07_RS20540 0.80 +0.8
2,782,225 + +3.0
2,782,225 + -0.2
2,782,299 + -0.7
2,782,299 + +0.5
2,782,299 + -1.5
2,782,299 + +0.6
2,782,300 - -1.5
2,782,669 + RPSI07_RS20545 0.25 -1.4
2,782,669 + RPSI07_RS20545 0.25 +0.5
2,782,670 - RPSI07_RS20545 0.25 -0.9
2,782,756 + RPSI07_RS20545 0.31 +1.5
2,782,828 + RPSI07_RS20545 0.36 +1.5

Or see this region's nucleotide sequence