Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS25215 and RPSI07_RS20530 are separated by 5 nucleotides RPSI07_RS20530 and RPSI07_RS20535 are separated by 86 nucleotides RPSI07_RS20535 and RPSI07_RS20540 are separated by 27 nucleotides RPSI07_RS20540 and RPSI07_RS20545 are separated by 69 nucleotides
RPSI07_RS25215: RPSI07_RS25215 - hypothetical protein, at 2,780,163 to 2,780,384
_RS25215
RPSI07_RS20530: RPSI07_RS20530 - TetR/AcrR family transcriptional regulator, at 2,780,390 to 2,781,133
_RS20530
RPSI07_RS20535: RPSI07_RS20535 - protein-L-isoaspartate O-methyltransferase, at 2,781,220 to 2,781,870
_RS20535
RPSI07_RS20540: RPSI07_RS20540 - sulfurtransferase, at 2,781,898 to 2,782,230
_RS20540
RPSI07_RS20545: RPSI07_RS20545 - channel protein TolC, at 2,782,300 to 2,783,754
_RS20545
Position (kb)
2781
2782 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2780.234 kb on - strand, within RPSI07_RS25215 at 2780.234 kb on - strand, within RPSI07_RS25215 at 2780.311 kb on - strand, within RPSI07_RS25215 at 2780.316 kb on - strand, within RPSI07_RS25215 at 2780.558 kb on + strand, within RPSI07_RS20530 at 2780.558 kb on + strand, within RPSI07_RS20530 at 2780.558 kb on + strand, within RPSI07_RS20530 at 2780.559 kb on - strand, within RPSI07_RS20530 at 2780.564 kb on + strand, within RPSI07_RS20530 at 2780.564 kb on + strand, within RPSI07_RS20530 at 2780.564 kb on + strand, within RPSI07_RS20530 at 2780.564 kb on + strand, within RPSI07_RS20530 at 2780.564 kb on + strand at 2780.565 kb on - strand, within RPSI07_RS20530 at 2780.565 kb on - strand, within RPSI07_RS20530 at 2780.565 kb on - strand, within RPSI07_RS20530 at 2780.565 kb on - strand, within RPSI07_RS20530 at 2780.786 kb on + strand, within RPSI07_RS20530 at 2780.786 kb on + strand, within RPSI07_RS20530 at 2780.786 kb on + strand, within RPSI07_RS20530 at 2780.786 kb on + strand, within RPSI07_RS20530 at 2780.789 kb on + strand, within RPSI07_RS20530 at 2781.170 kb on + strand at 2781.328 kb on + strand, within RPSI07_RS20535 at 2781.328 kb on + strand, within RPSI07_RS20535 at 2781.328 kb on + strand, within RPSI07_RS20535 at 2781.328 kb on + strand, within RPSI07_RS20535 at 2781.328 kb on + strand, within RPSI07_RS20535 at 2781.328 kb on + strand, within RPSI07_RS20535 at 2781.329 kb on - strand, within RPSI07_RS20535 at 2781.329 kb on - strand, within RPSI07_RS20535 at 2781.487 kb on + strand, within RPSI07_RS20535 at 2781.646 kb on + strand, within RPSI07_RS20535 at 2781.820 kb on + strand at 2781.820 kb on + strand at 2781.820 kb on + strand at 2781.820 kb on + strand at 2781.820 kb on + strand at 2782.166 kb on - strand, within RPSI07_RS20540 at 2782.225 kb on + strand at 2782.225 kb on + strand at 2782.299 kb on + strand at 2782.299 kb on + strand at 2782.299 kb on + strand at 2782.299 kb on + strand at 2782.300 kb on - strand at 2782.669 kb on + strand, within RPSI07_RS20545 at 2782.669 kb on + strand, within RPSI07_RS20545 at 2782.670 kb on - strand, within RPSI07_RS20545 at 2782.756 kb on + strand, within RPSI07_RS20545 at 2782.828 kb on + strand, within RPSI07_RS20545
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet remove 2,780,234 - RPSI07_RS25215 0.32 -1.9 2,780,234 - RPSI07_RS25215 0.32 -0.0 2,780,311 - RPSI07_RS25215 0.67 -0.8 2,780,316 - RPSI07_RS25215 0.69 -0.1 2,780,558 + RPSI07_RS20530 0.23 -0.7 2,780,558 + RPSI07_RS20530 0.23 +0.7 2,780,558 + RPSI07_RS20530 0.23 -0.4 2,780,559 - RPSI07_RS20530 0.23 +0.1 2,780,564 + RPSI07_RS20530 0.23 -0.3 2,780,564 + RPSI07_RS20530 0.23 +1.0 2,780,564 + RPSI07_RS20530 0.23 +0.1 2,780,564 + RPSI07_RS20530 0.23 +1.1 2,780,564 + +3.1 2,780,565 - RPSI07_RS20530 0.24 -0.3 2,780,565 - RPSI07_RS20530 0.24 -0.8 2,780,565 - RPSI07_RS20530 0.24 +0.1 2,780,565 - RPSI07_RS20530 0.24 -0.8 2,780,786 + RPSI07_RS20530 0.53 +2.1 2,780,786 + RPSI07_RS20530 0.53 +0.9 2,780,786 + RPSI07_RS20530 0.53 +0.9 2,780,786 + RPSI07_RS20530 0.53 +1.5 2,780,789 + RPSI07_RS20530 0.54 +0.6 2,781,170 + -0.0 2,781,328 + RPSI07_RS20535 0.17 +0.3 2,781,328 + RPSI07_RS20535 0.17 -0.5 2,781,328 + RPSI07_RS20535 0.17 +0.5 2,781,328 + RPSI07_RS20535 0.17 -0.1 2,781,328 + RPSI07_RS20535 0.17 -1.0 2,781,328 + RPSI07_RS20535 0.17 -1.4 2,781,329 - RPSI07_RS20535 0.17 -1.4 2,781,329 - RPSI07_RS20535 0.17 -0.1 2,781,487 + RPSI07_RS20535 0.41 -0.9 2,781,646 + RPSI07_RS20535 0.65 -1.3 2,781,820 + -1.7 2,781,820 + -0.3 2,781,820 + -1.2 2,781,820 + +1.5 2,781,820 + -0.5 2,782,166 - RPSI07_RS20540 0.80 +0.8 2,782,225 + +3.0 2,782,225 + -0.2 2,782,299 + -0.7 2,782,299 + +0.5 2,782,299 + -1.5 2,782,299 + +0.6 2,782,300 - -1.5 2,782,669 + RPSI07_RS20545 0.25 -1.4 2,782,669 + RPSI07_RS20545 0.25 +0.5 2,782,670 - RPSI07_RS20545 0.25 -0.9 2,782,756 + RPSI07_RS20545 0.31 +1.5 2,782,828 + RPSI07_RS20545 0.36 +1.5
Or see this region's nucleotide sequence