Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS16275 and RPSI07_RS16280 are separated by 42 nucleotides RPSI07_RS16280 and RPSI07_RS16285 overlap by 1 nucleotides RPSI07_RS16285 and RPSI07_RS16290 are separated by 22 nucleotides RPSI07_RS16290 and RPSI07_RS16295 are separated by 104 nucleotides
RPSI07_RS16275: RPSI07_RS16275 - peptidyl-prolyl cis-trans isomerase, at 1,811,758 to 1,812,555
_RS16275
RPSI07_RS16280: RPSI07_RS16280 - BolA family transcriptional regulator, at 1,812,598 to 1,812,870
_RS16280
RPSI07_RS16285: RPSI07_RS16285 - septation protein A, at 1,812,870 to 1,813,430
_RS16285
RPSI07_RS16290: RPSI07_RS16290 - peptide-methionine (R)-S-oxide reductase, at 1,813,453 to 1,813,839
_RS16290
RPSI07_RS16295: RPSI07_RS16295 - YdiU family protein, at 1,813,944 to 1,815,533
_RS16295
Position (kb)
1813
1814 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 1812.599 kb on + strand at 1812.600 kb on - strand at 1812.677 kb on + strand, within RPSI07_RS16280 at 1812.677 kb on + strand, within RPSI07_RS16280 at 1812.677 kb on + strand, within RPSI07_RS16280 at 1812.677 kb on + strand, within RPSI07_RS16280 at 1812.678 kb on - strand, within RPSI07_RS16280 at 1812.678 kb on - strand, within RPSI07_RS16280 at 1812.678 kb on - strand, within RPSI07_RS16280 at 1812.678 kb on - strand, within RPSI07_RS16280 at 1812.678 kb on - strand, within RPSI07_RS16280 at 1812.678 kb on - strand, within RPSI07_RS16280 at 1812.744 kb on - strand, within RPSI07_RS16280 at 1812.744 kb on - strand, within RPSI07_RS16280 at 1812.744 kb on - strand, within RPSI07_RS16280 at 1812.744 kb on - strand, within RPSI07_RS16280 at 1813.455 kb on - strand at 1813.468 kb on + strand at 1813.469 kb on - strand at 1813.490 kb on + strand at 1813.634 kb on + strand, within RPSI07_RS16290 at 1813.709 kb on + strand, within RPSI07_RS16290 at 1813.710 kb on - strand, within RPSI07_RS16290 at 1813.736 kb on + strand, within RPSI07_RS16290 at 1813.737 kb on - strand, within RPSI07_RS16290 at 1813.737 kb on - strand, within RPSI07_RS16290 at 1813.999 kb on + strand at 1814.000 kb on - strand at 1814.000 kb on - strand at 1814.125 kb on + strand, within RPSI07_RS16295 at 1814.125 kb on + strand, within RPSI07_RS16295 at 1814.126 kb on - strand, within RPSI07_RS16295 at 1814.126 kb on - strand, within RPSI07_RS16295 at 1814.323 kb on + strand, within RPSI07_RS16295 at 1814.323 kb on + strand, within RPSI07_RS16295 at 1814.324 kb on - strand, within RPSI07_RS16295 at 1814.324 kb on - strand, within RPSI07_RS16295 at 1814.425 kb on + strand, within RPSI07_RS16295 at 1814.426 kb on - strand, within RPSI07_RS16295 at 1814.440 kb on + strand, within RPSI07_RS16295 at 1814.440 kb on + strand, within RPSI07_RS16295 at 1814.543 kb on + strand, within RPSI07_RS16295 at 1814.620 kb on + strand, within RPSI07_RS16295 at 1814.620 kb on + strand, within RPSI07_RS16295 at 1814.621 kb on - strand, within RPSI07_RS16295 at 1814.824 kb on + strand, within RPSI07_RS16295 at 1814.824 kb on + strand, within RPSI07_RS16295 at 1814.825 kb on - strand, within RPSI07_RS16295
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet remove 1,812,599 + +1.2 1,812,600 - -0.6 1,812,677 + RPSI07_RS16280 0.29 +2.2 1,812,677 + RPSI07_RS16280 0.29 -0.5 1,812,677 + RPSI07_RS16280 0.29 -1.1 1,812,677 + RPSI07_RS16280 0.29 -0.1 1,812,678 - RPSI07_RS16280 0.29 +2.2 1,812,678 - RPSI07_RS16280 0.29 -0.9 1,812,678 - RPSI07_RS16280 0.29 +1.6 1,812,678 - RPSI07_RS16280 0.29 +0.6 1,812,678 - RPSI07_RS16280 0.29 -0.2 1,812,678 - RPSI07_RS16280 0.29 -0.7 1,812,744 - RPSI07_RS16280 0.53 -0.3 1,812,744 - RPSI07_RS16280 0.53 -0.3 1,812,744 - RPSI07_RS16280 0.53 +2.2 1,812,744 - RPSI07_RS16280 0.53 -1.1 1,813,455 - -2.1 1,813,468 + -1.4 1,813,469 - -0.9 1,813,490 + -0.6 1,813,634 + RPSI07_RS16290 0.47 +1.3 1,813,709 + RPSI07_RS16290 0.66 +0.1 1,813,710 - RPSI07_RS16290 0.66 -0.1 1,813,736 + RPSI07_RS16290 0.73 +0.6 1,813,737 - RPSI07_RS16290 0.73 -0.7 1,813,737 - RPSI07_RS16290 0.73 +0.2 1,813,999 + +0.4 1,814,000 - +0.5 1,814,000 - +4.5 1,814,125 + RPSI07_RS16295 0.11 -0.4 1,814,125 + RPSI07_RS16295 0.11 -1.4 1,814,126 - RPSI07_RS16295 0.11 -0.2 1,814,126 - RPSI07_RS16295 0.11 -1.0 1,814,323 + RPSI07_RS16295 0.24 -2.1 1,814,323 + RPSI07_RS16295 0.24 -2.1 1,814,324 - RPSI07_RS16295 0.24 -0.7 1,814,324 - RPSI07_RS16295 0.24 -0.3 1,814,425 + RPSI07_RS16295 0.30 +0.8 1,814,426 - RPSI07_RS16295 0.30 -0.5 1,814,440 + RPSI07_RS16295 0.31 -0.8 1,814,440 + RPSI07_RS16295 0.31 +2.8 1,814,543 + RPSI07_RS16295 0.38 +1.2 1,814,620 + RPSI07_RS16295 0.43 -2.0 1,814,620 + RPSI07_RS16295 0.43 -0.7 1,814,621 - RPSI07_RS16295 0.43 +1.9 1,814,824 + RPSI07_RS16295 0.55 -0.7 1,814,824 + RPSI07_RS16295 0.55 -0.0 1,814,825 - RPSI07_RS16295 0.55 -0.4
Or see this region's nucleotide sequence