Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS15790 and RPSI07_RS15795 are separated by 60 nucleotides RPSI07_RS15795 and RPSI07_RS15800 are separated by 107 nucleotides
RPSI07_RS15790: RPSI07_RS15790 - cytosine deaminase, at 1,697,444 to 1,698,715
_RS15790
RPSI07_RS15795: RPSI07_RS15795 - oligopeptidase A, at 1,698,776 to 1,700,896
_RS15795
RPSI07_RS15800: RPSI07_RS15800 - bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase FolD, at 1,701,004 to 1,701,873
_RS15800
Position (kb)
1698
1699
1700
1701 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1698.103 kb on + strand, within RPSI07_RS15790 at 1698.194 kb on + strand, within RPSI07_RS15790 at 1698.194 kb on + strand, within RPSI07_RS15790 at 1698.194 kb on + strand, within RPSI07_RS15790 at 1698.194 kb on + strand, within RPSI07_RS15790 at 1698.194 kb on + strand, within RPSI07_RS15790 at 1698.194 kb on + strand, within RPSI07_RS15790 at 1698.195 kb on - strand, within RPSI07_RS15790 at 1698.195 kb on - strand, within RPSI07_RS15790 at 1698.195 kb on - strand, within RPSI07_RS15790 at 1698.195 kb on - strand, within RPSI07_RS15790 at 1698.195 kb on - strand, within RPSI07_RS15790 at 1698.195 kb on - strand, within RPSI07_RS15790 at 1698.197 kb on + strand, within RPSI07_RS15790 at 1698.197 kb on + strand, within RPSI07_RS15790 at 1698.197 kb on + strand, within RPSI07_RS15790 at 1698.197 kb on + strand, within RPSI07_RS15790 at 1698.197 kb on + strand, within RPSI07_RS15790 at 1698.198 kb on - strand, within RPSI07_RS15790 at 1698.406 kb on + strand, within RPSI07_RS15790 at 1698.407 kb on - strand, within RPSI07_RS15790 at 1698.975 kb on + strand at 1699.008 kb on + strand, within RPSI07_RS15795 at 1699.008 kb on + strand, within RPSI07_RS15795 at 1699.009 kb on - strand, within RPSI07_RS15795 at 1699.009 kb on - strand, within RPSI07_RS15795 at 1699.014 kb on + strand, within RPSI07_RS15795 at 1699.015 kb on - strand, within RPSI07_RS15795 at 1699.015 kb on - strand, within RPSI07_RS15795 at 1699.163 kb on - strand, within RPSI07_RS15795 at 1699.163 kb on - strand, within RPSI07_RS15795 at 1699.615 kb on - strand, within RPSI07_RS15795 at 1699.810 kb on - strand, within RPSI07_RS15795 at 1699.839 kb on + strand, within RPSI07_RS15795 at 1699.840 kb on - strand, within RPSI07_RS15795 at 1699.840 kb on - strand, within RPSI07_RS15795 at 1700.184 kb on + strand, within RPSI07_RS15795 at 1700.184 kb on + strand, within RPSI07_RS15795 at 1700.203 kb on - strand, within RPSI07_RS15795 at 1700.335 kb on - strand, within RPSI07_RS15795 at 1700.509 kb on - strand, within RPSI07_RS15795 at 1700.535 kb on + strand, within RPSI07_RS15795 at 1700.629 kb on + strand, within RPSI07_RS15795 at 1701.005 kb on + strand at 1701.006 kb on - strand at 1701.006 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet remove 1,698,103 + RPSI07_RS15790 0.52 -0.3 1,698,194 + RPSI07_RS15790 0.59 -0.6 1,698,194 + RPSI07_RS15790 0.59 +1.2 1,698,194 + RPSI07_RS15790 0.59 +0.5 1,698,194 + RPSI07_RS15790 0.59 +0.6 1,698,194 + RPSI07_RS15790 0.59 -0.8 1,698,194 + RPSI07_RS15790 0.59 -2.4 1,698,195 - RPSI07_RS15790 0.59 -0.6 1,698,195 - RPSI07_RS15790 0.59 -0.7 1,698,195 - RPSI07_RS15790 0.59 +0.3 1,698,195 - RPSI07_RS15790 0.59 +0.4 1,698,195 - RPSI07_RS15790 0.59 +0.8 1,698,195 - RPSI07_RS15790 0.59 -0.4 1,698,197 + RPSI07_RS15790 0.59 -0.0 1,698,197 + RPSI07_RS15790 0.59 -3.0 1,698,197 + RPSI07_RS15790 0.59 -0.7 1,698,197 + RPSI07_RS15790 0.59 -0.9 1,698,197 + RPSI07_RS15790 0.59 -1.6 1,698,198 - RPSI07_RS15790 0.59 -0.7 1,698,406 + RPSI07_RS15790 0.76 +0.7 1,698,407 - RPSI07_RS15790 0.76 -0.8 1,698,975 + +0.6 1,699,008 + RPSI07_RS15795 0.11 -1.1 1,699,008 + RPSI07_RS15795 0.11 +0.8 1,699,009 - RPSI07_RS15795 0.11 -0.9 1,699,009 - RPSI07_RS15795 0.11 -1.4 1,699,014 + RPSI07_RS15795 0.11 +1.2 1,699,015 - RPSI07_RS15795 0.11 -0.8 1,699,015 - RPSI07_RS15795 0.11 -0.8 1,699,163 - RPSI07_RS15795 0.18 +0.6 1,699,163 - RPSI07_RS15795 0.18 -1.6 1,699,615 - RPSI07_RS15795 0.40 -0.2 1,699,810 - RPSI07_RS15795 0.49 -0.5 1,699,839 + RPSI07_RS15795 0.50 +0.6 1,699,840 - RPSI07_RS15795 0.50 -0.1 1,699,840 - RPSI07_RS15795 0.50 -1.9 1,700,184 + RPSI07_RS15795 0.66 -1.1 1,700,184 + RPSI07_RS15795 0.66 +0.2 1,700,203 - RPSI07_RS15795 0.67 -0.9 1,700,335 - RPSI07_RS15795 0.74 +1.1 1,700,509 - RPSI07_RS15795 0.82 +1.8 1,700,535 + RPSI07_RS15795 0.83 +0.2 1,700,629 + RPSI07_RS15795 0.87 -0.8 1,701,005 + +0.0 1,701,006 - -0.2 1,701,006 - -0.8
Or see this region's nucleotide sequence