Strain Fitness in Ralstonia solanacearum PSI07 around RPSI07_RS13260

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRPSI07_RS13250 and RPSI07_RS13255 are separated by 409 nucleotidesRPSI07_RS13255 and RPSI07_RS13260 are separated by 140 nucleotidesRPSI07_RS13260 and RPSI07_RS13265 overlap by 98 nucleotidesRPSI07_RS13265 and RPSI07_RS13270 overlap by 70 nucleotides RPSI07_RS13250: RPSI07_RS13250 - hypothetical protein, at 1,159,693 to 1,159,968 _RS13250 RPSI07_RS13255: RPSI07_RS13255 - hypothetical protein, at 1,160,378 to 1,160,617 _RS13255 RPSI07_RS13260: RPSI07_RS13260 - MerR family transcriptional regulator, at 1,160,758 to 1,161,288 _RS13260 RPSI07_RS13265: RPSI07_RS13265 - carboxymuconolactone decarboxylase, at 1,161,191 to 1,161,670 _RS13265 RPSI07_RS13270: RPSI07_RS13270 - HlyC/CorC family transporter, at 1,161,601 to 1,162,926 _RS13270 Position (kb) 1160 1161 1162Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 1159.990 kb on - strandat 1159.990 kb on - strandat 1159.990 kb on - strandat 1160.004 kb on + strandat 1160.004 kb on + strandat 1160.005 kb on - strandat 1160.005 kb on - strandat 1160.005 kb on - strandat 1160.099 kb on + strandat 1160.100 kb on - strandat 1160.100 kb on - strandat 1160.100 kb on - strandat 1160.110 kb on - strandat 1160.374 kb on + strandat 1160.374 kb on + strandat 1160.374 kb on + strandat 1160.374 kb on + strandat 1160.374 kb on + strandat 1160.374 kb on + strandat 1160.375 kb on - strandat 1160.739 kb on + strandat 1160.746 kb on + strandat 1160.746 kb on + strandat 1160.746 kb on + strandat 1160.746 kb on + strandat 1160.746 kb on + strandat 1160.747 kb on - strandat 1160.747 kb on - strandat 1160.747 kb on - strandat 1160.828 kb on - strand, within RPSI07_RS13260at 1161.109 kb on + strand, within RPSI07_RS13260at 1161.110 kb on - strand, within RPSI07_RS13260at 1161.110 kb on - strand, within RPSI07_RS13260at 1161.110 kb on - strand, within RPSI07_RS13260at 1161.110 kb on - strand, within RPSI07_RS13260at 1161.252 kb on - strandat 1161.347 kb on + strand, within RPSI07_RS13265at 1161.347 kb on + strand, within RPSI07_RS13265at 1161.347 kb on + strand, within RPSI07_RS13265at 1161.347 kb on + strand, within RPSI07_RS13265at 1161.347 kb on + strand, within RPSI07_RS13265at 1161.348 kb on - strand, within RPSI07_RS13265at 1161.349 kb on + strand, within RPSI07_RS13265at 1161.349 kb on + strand, within RPSI07_RS13265at 1161.349 kb on + strand, within RPSI07_RS13265at 1161.377 kb on + strand, within RPSI07_RS13265at 1161.377 kb on + strand, within RPSI07_RS13265at 1161.378 kb on - strand, within RPSI07_RS13265at 1161.378 kb on - strand, within RPSI07_RS13265at 1161.378 kb on - strand, within RPSI07_RS13265at 1161.379 kb on - strand, within RPSI07_RS13265at 1161.542 kb on + strand, within RPSI07_RS13265at 1161.542 kb on + strand, within RPSI07_RS13265at 1161.543 kb on - strand, within RPSI07_RS13265at 1161.761 kb on + strand, within RPSI07_RS13270at 1161.761 kb on + strand, within RPSI07_RS13270at 1161.762 kb on - strand, within RPSI07_RS13270at 1161.762 kb on - strand, within RPSI07_RS13270at 1161.911 kb on + strand, within RPSI07_RS13270at 1161.981 kb on - strand, within RPSI07_RS13270at 1162.080 kb on - strand, within RPSI07_RS13270at 1162.196 kb on + strand, within RPSI07_RS13270at 1162.197 kb on - strand, within RPSI07_RS13270at 1162.262 kb on + strand, within RPSI07_RS13270at 1162.263 kb on - strand, within RPSI07_RS13270

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet
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1,159,990 - -0.6
1,159,990 - -1.0
1,159,990 - -1.8
1,160,004 + -0.5
1,160,004 + -1.6
1,160,005 - -2.1
1,160,005 - +0.2
1,160,005 - +0.5
1,160,099 + -0.1
1,160,100 - -1.2
1,160,100 - +0.7
1,160,100 - -0.2
1,160,110 - -1.1
1,160,374 + -0.3
1,160,374 + -1.2
1,160,374 + +0.9
1,160,374 + +0.2
1,160,374 + -2.6
1,160,374 + +0.1
1,160,375 - +2.9
1,160,739 + -0.7
1,160,746 + +1.4
1,160,746 + -1.6
1,160,746 + -0.0
1,160,746 + -0.9
1,160,746 + -1.0
1,160,747 - -0.2
1,160,747 - +2.0
1,160,747 - -1.5
1,160,828 - RPSI07_RS13260 0.13 +0.7
1,161,109 + RPSI07_RS13260 0.66 +0.5
1,161,110 - RPSI07_RS13260 0.66 +1.0
1,161,110 - RPSI07_RS13260 0.66 +0.3
1,161,110 - RPSI07_RS13260 0.66 -0.2
1,161,110 - RPSI07_RS13260 0.66 +0.2
1,161,252 - -1.1
1,161,347 + RPSI07_RS13265 0.33 +0.6
1,161,347 + RPSI07_RS13265 0.33 -0.2
1,161,347 + RPSI07_RS13265 0.33 -2.2
1,161,347 + RPSI07_RS13265 0.33 +0.2
1,161,347 + RPSI07_RS13265 0.33 -1.9
1,161,348 - RPSI07_RS13265 0.33 +0.7
1,161,349 + RPSI07_RS13265 0.33 -0.6
1,161,349 + RPSI07_RS13265 0.33 -1.7
1,161,349 + RPSI07_RS13265 0.33 +0.5
1,161,377 + RPSI07_RS13265 0.39 -2.1
1,161,377 + RPSI07_RS13265 0.39 -1.1
1,161,378 - RPSI07_RS13265 0.39 -0.6
1,161,378 - RPSI07_RS13265 0.39 +1.9
1,161,378 - RPSI07_RS13265 0.39 +0.4
1,161,379 - RPSI07_RS13265 0.39 +0.1
1,161,542 + RPSI07_RS13265 0.73 +0.2
1,161,542 + RPSI07_RS13265 0.73 +0.7
1,161,543 - RPSI07_RS13265 0.73 +0.4
1,161,761 + RPSI07_RS13270 0.12 +1.4
1,161,761 + RPSI07_RS13270 0.12 -0.5
1,161,762 - RPSI07_RS13270 0.12 +2.9
1,161,762 - RPSI07_RS13270 0.12 +0.5
1,161,911 + RPSI07_RS13270 0.23 -1.2
1,161,981 - RPSI07_RS13270 0.29 -0.8
1,162,080 - RPSI07_RS13270 0.36 +0.4
1,162,196 + RPSI07_RS13270 0.45 -1.3
1,162,197 - RPSI07_RS13270 0.45 -0.2
1,162,262 + RPSI07_RS13270 0.50 -2.9
1,162,263 - RPSI07_RS13270 0.50 -2.5

Or see this region's nucleotide sequence