Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS12175 and RPSI07_RS12180 are separated by 25 nucleotides RPSI07_RS12180 and RPSI07_RS12185 overlap by 7 nucleotides RPSI07_RS12185 and RPSI07_RS12190 are separated by 48 nucleotides
RPSI07_RS12175: RPSI07_RS12175 - tRNA epoxyqueuosine(34) reductase QueG, at 930,508 to 931,659
_RS12175
RPSI07_RS12180: RPSI07_RS12180 - tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex ATPase subunit type 1 TsaE, at 931,685 to 932,254
_RS12180
RPSI07_RS12185: RPSI07_RS12185 - N-acetylmuramoyl-L-alanine amidase, at 932,248 to 933,771
_RS12185
RPSI07_RS12190: RPSI07_RS12190 - bile acid:sodium symporter, at 933,820 to 934,839
_RS12190
Position (kb)
932
933
934 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 931.266 kb on - strand, within RPSI07_RS12175 at 931.364 kb on + strand, within RPSI07_RS12175 at 931.364 kb on + strand, within RPSI07_RS12175 at 931.364 kb on + strand, within RPSI07_RS12175 at 931.364 kb on + strand, within RPSI07_RS12175 at 931.364 kb on + strand, within RPSI07_RS12175 at 931.365 kb on - strand, within RPSI07_RS12175 at 931.365 kb on - strand, within RPSI07_RS12175 at 931.365 kb on - strand, within RPSI07_RS12175 at 931.365 kb on - strand, within RPSI07_RS12175 at 931.445 kb on + strand, within RPSI07_RS12175 at 931.446 kb on + strand, within RPSI07_RS12175 at 931.446 kb on + strand, within RPSI07_RS12175 at 931.446 kb on + strand, within RPSI07_RS12175 at 931.446 kb on + strand, within RPSI07_RS12175 at 931.447 kb on - strand, within RPSI07_RS12175 at 931.447 kb on - strand, within RPSI07_RS12175 at 931.447 kb on - strand, within RPSI07_RS12175 at 931.447 kb on - strand, within RPSI07_RS12175 at 931.447 kb on - strand, within RPSI07_RS12175 at 931.448 kb on + strand, within RPSI07_RS12175 at 931.448 kb on + strand at 931.448 kb on + strand, within RPSI07_RS12175 at 931.448 kb on + strand, within RPSI07_RS12175 at 931.663 kb on + strand at 931.663 kb on + strand at 931.670 kb on + strand at 932.431 kb on + strand, within RPSI07_RS12185 at 932.432 kb on - strand, within RPSI07_RS12185 at 932.581 kb on + strand, within RPSI07_RS12185 at 932.581 kb on + strand, within RPSI07_RS12185 at 932.582 kb on - strand, within RPSI07_RS12185 at 932.719 kb on + strand, within RPSI07_RS12185 at 933.233 kb on - strand, within RPSI07_RS12185 at 933.233 kb on - strand, within RPSI07_RS12185 at 933.683 kb on - strand at 933.926 kb on + strand, within RPSI07_RS12190 at 933.926 kb on + strand, within RPSI07_RS12190 at 933.926 kb on + strand, within RPSI07_RS12190 at 933.927 kb on - strand, within RPSI07_RS12190 at 933.927 kb on - strand, within RPSI07_RS12190 at 933.927 kb on - strand, within RPSI07_RS12190 at 933.927 kb on - strand, within RPSI07_RS12190 at 933.927 kb on - strand, within RPSI07_RS12190 at 933.927 kb on - strand, within RPSI07_RS12190 at 934.154 kb on + strand, within RPSI07_RS12190 at 934.155 kb on - strand, within RPSI07_RS12190 at 934.250 kb on + strand, within RPSI07_RS12190 at 934.250 kb on + strand, within RPSI07_RS12190 at 934.250 kb on + strand, within RPSI07_RS12190 at 934.250 kb on + strand, within RPSI07_RS12190 at 934.250 kb on + strand, within RPSI07_RS12190 at 934.251 kb on - strand, within RPSI07_RS12190 at 934.251 kb on - strand, within RPSI07_RS12190 at 934.251 kb on - strand, within RPSI07_RS12190 at 934.251 kb on - strand, within RPSI07_RS12190 at 934.251 kb on - strand, within RPSI07_RS12190 at 934.437 kb on - strand, within RPSI07_RS12190 at 934.517 kb on + strand, within RPSI07_RS12190 at 934.518 kb on - strand, within RPSI07_RS12190
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet remove 931,266 - RPSI07_RS12175 0.66 +1.4 931,364 + RPSI07_RS12175 0.74 -1.4 931,364 + RPSI07_RS12175 0.74 +1.4 931,364 + RPSI07_RS12175 0.74 +0.0 931,364 + RPSI07_RS12175 0.74 +0.4 931,364 + RPSI07_RS12175 0.74 -0.2 931,365 - RPSI07_RS12175 0.74 -1.2 931,365 - RPSI07_RS12175 0.74 +0.6 931,365 - RPSI07_RS12175 0.74 -1.2 931,365 - RPSI07_RS12175 0.74 -2.6 931,445 + RPSI07_RS12175 0.81 -1.2 931,446 + RPSI07_RS12175 0.81 -0.5 931,446 + RPSI07_RS12175 0.81 -0.2 931,446 + RPSI07_RS12175 0.81 -1.8 931,446 + RPSI07_RS12175 0.81 -0.2 931,447 - RPSI07_RS12175 0.82 +0.4 931,447 - RPSI07_RS12175 0.82 +1.4 931,447 - RPSI07_RS12175 0.82 -1.3 931,447 - RPSI07_RS12175 0.82 -2.2 931,447 - RPSI07_RS12175 0.82 -2.8 931,448 + RPSI07_RS12175 0.82 +3.0 931,448 + -2.3 931,448 + RPSI07_RS12175 0.82 -0.5 931,448 + RPSI07_RS12175 0.82 +2.1 931,663 + +0.1 931,663 + -0.1 931,670 + +0.3 932,431 + RPSI07_RS12185 0.12 +2.7 932,432 - RPSI07_RS12185 0.12 +0.4 932,581 + RPSI07_RS12185 0.22 +3.0 932,581 + RPSI07_RS12185 0.22 -0.9 932,582 - RPSI07_RS12185 0.22 -0.6 932,719 + RPSI07_RS12185 0.31 +0.4 933,233 - RPSI07_RS12185 0.65 +0.4 933,233 - RPSI07_RS12185 0.65 +0.2 933,683 - +1.8 933,926 + RPSI07_RS12190 0.10 -1.2 933,926 + RPSI07_RS12190 0.10 +0.3 933,926 + RPSI07_RS12190 0.10 +1.4 933,927 - RPSI07_RS12190 0.10 -0.5 933,927 - RPSI07_RS12190 0.10 -0.9 933,927 - RPSI07_RS12190 0.10 +0.4 933,927 - RPSI07_RS12190 0.10 +0.4 933,927 - RPSI07_RS12190 0.10 +0.8 933,927 - RPSI07_RS12190 0.10 -1.0 934,154 + RPSI07_RS12190 0.33 +1.7 934,155 - RPSI07_RS12190 0.33 -2.2 934,250 + RPSI07_RS12190 0.42 -1.9 934,250 + RPSI07_RS12190 0.42 +0.2 934,250 + RPSI07_RS12190 0.42 +2.3 934,250 + RPSI07_RS12190 0.42 -0.6 934,250 + RPSI07_RS12190 0.42 -0.4 934,251 - RPSI07_RS12190 0.42 -1.5 934,251 - RPSI07_RS12190 0.42 -0.7 934,251 - RPSI07_RS12190 0.42 -1.3 934,251 - RPSI07_RS12190 0.42 -1.2 934,251 - RPSI07_RS12190 0.42 -0.0 934,437 - RPSI07_RS12190 0.60 -2.4 934,517 + RPSI07_RS12190 0.68 -2.7 934,518 - RPSI07_RS12190 0.68 -2.0
Or see this region's nucleotide sequence