Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS11065 and RPSI07_RS11070 are separated by 175 nucleotides RPSI07_RS11070 and RPSI07_RS11075 are separated by 26 nucleotides RPSI07_RS11075 and RPSI07_RS24935 are separated by 278 nucleotides RPSI07_RS24935 and RPSI07_RS11085 are separated by 37 nucleotides
RPSI07_RS11065: RPSI07_RS11065 - HlyC/CorC family transporter, at 684,459 to 685,736
_RS11065
RPSI07_RS11070: RPSI07_RS11070 - tRNA-Pro, at 685,912 to 685,988
_RS11070
RPSI07_RS11075: RPSI07_RS11075 - octaprenyl diphosphate synthase, at 686,015 to 686,983
_RS11075
RPSI07_RS24935: RPSI07_RS24935 - 50S ribosomal protein L21, at 687,262 to 687,573
_RS24935
RPSI07_RS11085: RPSI07_RS11085 - 50S ribosomal protein L27, at 687,611 to 687,871
_RS11085
Position (kb) 686
687 Strain fitness (log2 ratio) -2
-1
0
1
2 at 685.030 kb on + strand, within RPSI07_RS11065 at 685.030 kb on + strand, within RPSI07_RS11065 at 685.372 kb on + strand, within RPSI07_RS11065 at 685.474 kb on + strand, within RPSI07_RS11065 at 685.475 kb on - strand, within RPSI07_RS11065 at 685.768 kb on + strand at 685.769 kb on - strand at 685.770 kb on + strand at 685.771 kb on - strand at 685.771 kb on - strand at 685.771 kb on - strand at 685.771 kb on - strand at 687.036 kb on - strand at 687.067 kb on - strand at 687.067 kb on - strand at 687.944 kb on + strand at 687.944 kb on + strand at 687.960 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet remove 685,030 + RPSI07_RS11065 0.45 -1.0 685,030 + RPSI07_RS11065 0.45 -1.5 685,372 + RPSI07_RS11065 0.71 +1.1 685,474 + RPSI07_RS11065 0.79 -0.7 685,475 - RPSI07_RS11065 0.79 +0.8 685,768 + +0.7 685,769 - -0.1 685,770 + -1.9 685,771 - +1.7 685,771 - -0.7 685,771 - +0.0 685,771 - -0.9 687,036 - +2.5 687,067 - -1.1 687,067 - +0.9 687,944 + +0.1 687,944 + +0.1 687,960 + -0.4
Or see this region's nucleotide sequence