Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS05250 and RPSI07_RS05255 are separated by 273 nucleotides RPSI07_RS05255 and RPSI07_RS05260 are separated by 132 nucleotides
RPSI07_RS05250: RPSI07_RS05250 - amino acid ABC transporter substrate-binding protein, at 1,438,833 to 1,439,723
_RS05250
RPSI07_RS05255: RPSI07_RS05255 - DNA-binding response regulator, at 1,439,997 to 1,440,524
_RS05255
RPSI07_RS05260: RPSI07_RS05260 - LuxR family transcriptional regulator, at 1,440,657 to 1,441,697
_RS05260
Position (kb)
1439
1440
1441 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1439.041 kb on + strand, within RPSI07_RS05250 at 1439.042 kb on - strand, within RPSI07_RS05250 at 1439.350 kb on + strand, within RPSI07_RS05250 at 1439.350 kb on + strand, within RPSI07_RS05250 at 1439.350 kb on + strand, within RPSI07_RS05250 at 1439.396 kb on + strand, within RPSI07_RS05250 at 1439.473 kb on + strand, within RPSI07_RS05250 at 1439.473 kb on + strand, within RPSI07_RS05250 at 1439.548 kb on + strand, within RPSI07_RS05250 at 1439.548 kb on + strand, within RPSI07_RS05250 at 1439.548 kb on + strand, within RPSI07_RS05250 at 1439.549 kb on - strand, within RPSI07_RS05250 at 1439.578 kb on + strand, within RPSI07_RS05250 at 1439.579 kb on - strand, within RPSI07_RS05250 at 1439.579 kb on - strand, within RPSI07_RS05250 at 1439.766 kb on - strand at 1439.766 kb on - strand at 1439.766 kb on - strand at 1439.795 kb on + strand at 1439.795 kb on + strand at 1440.034 kb on + strand at 1440.034 kb on + strand at 1440.034 kb on + strand at 1440.034 kb on + strand at 1440.034 kb on + strand at 1440.034 kb on + strand at 1440.035 kb on - strand at 1440.035 kb on - strand at 1440.092 kb on + strand, within RPSI07_RS05255 at 1440.092 kb on + strand, within RPSI07_RS05255 at 1440.093 kb on - strand, within RPSI07_RS05255 at 1440.093 kb on - strand, within RPSI07_RS05255 at 1440.093 kb on - strand, within RPSI07_RS05255 at 1440.094 kb on - strand, within RPSI07_RS05255 at 1440.119 kb on + strand, within RPSI07_RS05255 at 1440.120 kb on - strand, within RPSI07_RS05255 at 1440.120 kb on - strand, within RPSI07_RS05255 at 1440.120 kb on - strand, within RPSI07_RS05255 at 1440.120 kb on - strand, within RPSI07_RS05255 at 1440.329 kb on + strand, within RPSI07_RS05255 at 1440.330 kb on - strand, within RPSI07_RS05255 at 1440.330 kb on - strand, within RPSI07_RS05255 at 1440.527 kb on + strand at 1440.527 kb on + strand at 1440.527 kb on + strand at 1440.527 kb on + strand at 1440.527 kb on + strand at 1440.527 kb on + strand at 1440.527 kb on + strand at 1440.527 kb on + strand at 1440.527 kb on + strand at 1440.527 kb on + strand at 1440.527 kb on + strand at 1440.528 kb on - strand at 1440.528 kb on - strand at 1440.528 kb on - strand at 1440.528 kb on - strand at 1440.528 kb on - strand at 1440.528 kb on - strand at 1440.531 kb on + strand at 1440.531 kb on + strand at 1440.531 kb on + strand at 1440.531 kb on + strand at 1440.531 kb on + strand at 1440.532 kb on - strand at 1440.532 kb on - strand at 1440.532 kb on - strand at 1440.532 kb on - strand at 1440.532 kb on - strand at 1440.532 kb on - strand at 1440.537 kb on + strand at 1440.537 kb on + strand at 1440.537 kb on + strand at 1440.537 kb on + strand at 1440.537 kb on + strand at 1440.538 kb on - strand at 1440.538 kb on - strand at 1440.538 kb on - strand at 1440.538 kb on - strand at 1440.558 kb on + strand at 1440.559 kb on - strand at 1440.588 kb on - strand at 1440.600 kb on - strand at 1440.775 kb on + strand, within RPSI07_RS05260 at 1440.775 kb on + strand, within RPSI07_RS05260 at 1440.776 kb on - strand, within RPSI07_RS05260 at 1440.776 kb on - strand, within RPSI07_RS05260 at 1440.899 kb on + strand, within RPSI07_RS05260 at 1441.318 kb on + strand, within RPSI07_RS05260 at 1441.318 kb on + strand, within RPSI07_RS05260 at 1441.318 kb on + strand, within RPSI07_RS05260 at 1441.318 kb on + strand, within RPSI07_RS05260 at 1441.319 kb on - strand, within RPSI07_RS05260 at 1441.319 kb on - strand, within RPSI07_RS05260 at 1441.319 kb on - strand, within RPSI07_RS05260 at 1441.319 kb on - strand, within RPSI07_RS05260 at 1441.319 kb on - strand, within RPSI07_RS05260 at 1441.319 kb on - strand, within RPSI07_RS05260 at 1441.322 kb on + strand, within RPSI07_RS05260 at 1441.322 kb on + strand, within RPSI07_RS05260 at 1441.322 kb on + strand, within RPSI07_RS05260 at 1441.322 kb on + strand, within RPSI07_RS05260 at 1441.322 kb on + strand, within RPSI07_RS05260 at 1441.322 kb on + strand, within RPSI07_RS05260 at 1441.322 kb on + strand, within RPSI07_RS05260 at 1441.322 kb on + strand, within RPSI07_RS05260 at 1441.322 kb on + strand, within RPSI07_RS05260 at 1441.322 kb on + strand, within RPSI07_RS05260 at 1441.323 kb on - strand, within RPSI07_RS05260 at 1441.323 kb on - strand, within RPSI07_RS05260 at 1441.323 kb on - strand, within RPSI07_RS05260 at 1441.323 kb on - strand, within RPSI07_RS05260 at 1441.323 kb on - strand, within RPSI07_RS05260 at 1441.323 kb on - strand, within RPSI07_RS05260 at 1441.323 kb on - strand, within RPSI07_RS05260 at 1441.323 kb on - strand, within RPSI07_RS05260 at 1441.323 kb on - strand, within RPSI07_RS05260 at 1441.323 kb on - strand, within RPSI07_RS05260 at 1441.336 kb on + strand, within RPSI07_RS05260 at 1441.337 kb on - strand, within RPSI07_RS05260 at 1441.337 kb on - strand, within RPSI07_RS05260 at 1441.370 kb on - strand, within RPSI07_RS05260 at 1441.370 kb on - strand, within RPSI07_RS05260 at 1441.481 kb on - strand, within RPSI07_RS05260
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet remove 1,439,041 + RPSI07_RS05250 0.23 +0.6 1,439,042 - RPSI07_RS05250 0.23 +0.3 1,439,350 + RPSI07_RS05250 0.58 +0.2 1,439,350 + RPSI07_RS05250 0.58 -2.3 1,439,350 + RPSI07_RS05250 0.58 +2.0 1,439,396 + RPSI07_RS05250 0.63 +1.3 1,439,473 + RPSI07_RS05250 0.72 +1.4 1,439,473 + RPSI07_RS05250 0.72 -1.4 1,439,548 + RPSI07_RS05250 0.80 +1.3 1,439,548 + RPSI07_RS05250 0.80 +3.3 1,439,548 + RPSI07_RS05250 0.80 -0.9 1,439,549 - RPSI07_RS05250 0.80 -1.0 1,439,578 + RPSI07_RS05250 0.84 -0.1 1,439,579 - RPSI07_RS05250 0.84 -1.2 1,439,579 - RPSI07_RS05250 0.84 +1.0 1,439,766 - -1.1 1,439,766 - +0.5 1,439,766 - -1.1 1,439,795 + +2.3 1,439,795 + +0.2 1,440,034 + -2.0 1,440,034 + +0.0 1,440,034 + +0.1 1,440,034 + +0.5 1,440,034 + +0.9 1,440,034 + +0.5 1,440,035 - -0.5 1,440,035 - +1.1 1,440,092 + RPSI07_RS05255 0.18 -1.5 1,440,092 + RPSI07_RS05255 0.18 -0.9 1,440,093 - RPSI07_RS05255 0.18 +0.3 1,440,093 - RPSI07_RS05255 0.18 -1.7 1,440,093 - RPSI07_RS05255 0.18 -2.3 1,440,094 - RPSI07_RS05255 0.18 +0.3 1,440,119 + RPSI07_RS05255 0.23 -0.6 1,440,120 - RPSI07_RS05255 0.23 -1.5 1,440,120 - RPSI07_RS05255 0.23 +0.0 1,440,120 - RPSI07_RS05255 0.23 +0.7 1,440,120 - RPSI07_RS05255 0.23 -0.7 1,440,329 + RPSI07_RS05255 0.63 -0.4 1,440,330 - RPSI07_RS05255 0.63 +3.2 1,440,330 - RPSI07_RS05255 0.63 -0.8 1,440,527 + +0.2 1,440,527 + +0.5 1,440,527 + +1.3 1,440,527 + +0.9 1,440,527 + -0.3 1,440,527 + +0.0 1,440,527 + -0.0 1,440,527 + +1.9 1,440,527 + -1.2 1,440,527 + +0.1 1,440,527 + -0.2 1,440,528 - -1.0 1,440,528 - +0.8 1,440,528 - -1.5 1,440,528 - +0.1 1,440,528 - -0.5 1,440,528 - -0.6 1,440,531 + -0.4 1,440,531 + -1.1 1,440,531 + +0.2 1,440,531 + +1.0 1,440,531 + -0.4 1,440,532 - +0.3 1,440,532 - +0.7 1,440,532 - -0.3 1,440,532 - -0.3 1,440,532 - +0.2 1,440,532 - +2.3 1,440,537 + -0.6 1,440,537 + +0.1 1,440,537 + -0.7 1,440,537 + -0.1 1,440,537 + -0.4 1,440,538 - +0.3 1,440,538 - -0.1 1,440,538 - +0.1 1,440,538 - +0.9 1,440,558 + -0.3 1,440,559 - +2.0 1,440,588 - -1.1 1,440,600 - +0.5 1,440,775 + RPSI07_RS05260 0.11 -0.7 1,440,775 + RPSI07_RS05260 0.11 -0.7 1,440,776 - RPSI07_RS05260 0.11 +0.7 1,440,776 - RPSI07_RS05260 0.11 -0.0 1,440,899 + RPSI07_RS05260 0.23 -0.4 1,441,318 + RPSI07_RS05260 0.63 -1.7 1,441,318 + RPSI07_RS05260 0.63 -0.7 1,441,318 + RPSI07_RS05260 0.63 -1.9 1,441,318 + RPSI07_RS05260 0.63 -0.1 1,441,319 - RPSI07_RS05260 0.64 +0.4 1,441,319 - RPSI07_RS05260 0.64 +0.5 1,441,319 - RPSI07_RS05260 0.64 -1.0 1,441,319 - RPSI07_RS05260 0.64 -1.3 1,441,319 - RPSI07_RS05260 0.64 +0.7 1,441,319 - RPSI07_RS05260 0.64 -0.1 1,441,322 + RPSI07_RS05260 0.64 +0.4 1,441,322 + RPSI07_RS05260 0.64 +0.7 1,441,322 + RPSI07_RS05260 0.64 -2.3 1,441,322 + RPSI07_RS05260 0.64 +0.3 1,441,322 + RPSI07_RS05260 0.64 +1.3 1,441,322 + RPSI07_RS05260 0.64 -1.8 1,441,322 + RPSI07_RS05260 0.64 -0.7 1,441,322 + RPSI07_RS05260 0.64 -0.5 1,441,322 + RPSI07_RS05260 0.64 -0.7 1,441,322 + RPSI07_RS05260 0.64 -1.4 1,441,323 - RPSI07_RS05260 0.64 -1.5 1,441,323 - RPSI07_RS05260 0.64 +3.3 1,441,323 - RPSI07_RS05260 0.64 -1.1 1,441,323 - RPSI07_RS05260 0.64 -0.4 1,441,323 - RPSI07_RS05260 0.64 +0.1 1,441,323 - RPSI07_RS05260 0.64 -0.3 1,441,323 - RPSI07_RS05260 0.64 -0.3 1,441,323 - RPSI07_RS05260 0.64 +1.3 1,441,323 - RPSI07_RS05260 0.64 +0.2 1,441,323 - RPSI07_RS05260 0.64 +0.4 1,441,336 + RPSI07_RS05260 0.65 -0.0 1,441,337 - RPSI07_RS05260 0.65 +2.3 1,441,337 - RPSI07_RS05260 0.65 -1.9 1,441,370 - RPSI07_RS05260 0.68 -1.1 1,441,370 - RPSI07_RS05260 0.68 -0.9 1,441,481 - RPSI07_RS05260 0.79 +0.5
Or see this region's nucleotide sequence