Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS03515 and RPSI07_RS03520 are separated by 63 nucleotides RPSI07_RS03520 and RPSI07_RS03525 are separated by 192 nucleotides RPSI07_RS03525 and RPSI07_RS24325 are separated by 329 nucleotides
RPSI07_RS03515: RPSI07_RS03515 - nicotinate phosphoribosyltransferase, at 955,861 to 957,312
_RS03515
RPSI07_RS03520: RPSI07_RS03520 - hypothetical protein, at 957,376 to 957,888
_RS03520
RPSI07_RS03525: RPSI07_RS03525 - sodium transporter, at 958,081 to 958,869
_RS03525
RPSI07_RS24325: RPSI07_RS24325 - hypothetical protein, at 959,199 to 959,930
_RS24325
Position (kb) 958
959 Strain fitness (log2 ratio) -2
-1
0
1
2 at 957.142 kb on + strand, within RPSI07_RS03515 at 957.142 kb on + strand, within RPSI07_RS03515 at 957.143 kb on - strand, within RPSI07_RS03515 at 957.154 kb on - strand, within RPSI07_RS03515 at 957.284 kb on - strand at 957.284 kb on - strand at 957.425 kb on + strand at 958.059 kb on + strand at 958.059 kb on + strand at 958.059 kb on + strand at 958.060 kb on - strand at 958.060 kb on - strand at 958.060 kb on - strand at 958.060 kb on - strand at 958.066 kb on + strand at 958.075 kb on + strand at 958.075 kb on + strand at 958.075 kb on + strand at 958.075 kb on + strand at 958.075 kb on + strand at 958.075 kb on + strand at 958.076 kb on - strand at 958.076 kb on - strand at 958.076 kb on - strand at 958.076 kb on - strand at 958.076 kb on - strand at 958.076 kb on - strand at 958.076 kb on - strand at 958.076 kb on - strand at 958.076 kb on - strand at 958.076 kb on - strand at 958.122 kb on + strand at 958.124 kb on - strand at 958.130 kb on - strand at 958.156 kb on + strand at 958.156 kb on + strand at 958.157 kb on - strand at 958.180 kb on + strand, within RPSI07_RS03525 at 958.180 kb on + strand, within RPSI07_RS03525 at 958.180 kb on + strand, within RPSI07_RS03525 at 958.181 kb on - strand, within RPSI07_RS03525 at 958.181 kb on - strand, within RPSI07_RS03525 at 958.181 kb on - strand, within RPSI07_RS03525 at 958.303 kb on - strand, within RPSI07_RS03525 at 958.303 kb on - strand, within RPSI07_RS03525 at 958.312 kb on + strand, within RPSI07_RS03525 at 958.486 kb on + strand, within RPSI07_RS03525 at 958.486 kb on + strand, within RPSI07_RS03525 at 958.486 kb on + strand, within RPSI07_RS03525 at 958.486 kb on + strand, within RPSI07_RS03525 at 958.487 kb on - strand, within RPSI07_RS03525 at 958.487 kb on - strand, within RPSI07_RS03525 at 958.487 kb on - strand, within RPSI07_RS03525 at 958.487 kb on - strand, within RPSI07_RS03525 at 958.574 kb on + strand, within RPSI07_RS03525 at 958.575 kb on - strand, within RPSI07_RS03525 at 958.672 kb on + strand, within RPSI07_RS03525 at 958.672 kb on + strand, within RPSI07_RS03525 at 958.672 kb on + strand, within RPSI07_RS03525 at 958.673 kb on - strand, within RPSI07_RS03525 at 958.673 kb on - strand, within RPSI07_RS03525 at 958.673 kb on - strand, within RPSI07_RS03525 at 958.673 kb on - strand, within RPSI07_RS03525 at 958.673 kb on - strand, within RPSI07_RS03525 at 958.673 kb on - strand, within RPSI07_RS03525 at 958.717 kb on + strand, within RPSI07_RS03525 at 958.717 kb on + strand, within RPSI07_RS03525 at 958.718 kb on - strand, within RPSI07_RS03525 at 958.718 kb on - strand, within RPSI07_RS03525 at 958.752 kb on + strand, within RPSI07_RS03525 at 958.753 kb on - strand, within RPSI07_RS03525 at 958.755 kb on - strand, within RPSI07_RS03525 at 958.759 kb on + strand, within RPSI07_RS03525 at 958.951 kb on + strand at 958.952 kb on + strand at 958.952 kb on + strand at 958.952 kb on + strand at 958.952 kb on + strand at 958.952 kb on + strand at 958.952 kb on + strand at 958.952 kb on + strand at 958.952 kb on + strand at 958.953 kb on - strand at 958.953 kb on - strand at 958.953 kb on - strand at 958.953 kb on - strand at 958.953 kb on - strand at 958.953 kb on - strand at 958.953 kb on - strand at 958.953 kb on - strand at 958.953 kb on - strand at 958.953 kb on - strand at 958.953 kb on - strand at 958.953 kb on - strand at 958.953 kb on - strand at 958.953 kb on - strand at 958.953 kb on - strand at 958.953 kb on - strand at 958.953 kb on - strand at 958.953 kb on - strand at 959.097 kb on + strand at 959.097 kb on + strand at 959.097 kb on + strand at 959.098 kb on - strand at 959.098 kb on - strand at 959.098 kb on - strand at 959.098 kb on - strand at 959.098 kb on - strand at 959.098 kb on - strand at 959.513 kb on + strand, within RPSI07_RS24325 at 959.513 kb on + strand, within RPSI07_RS24325 at 959.513 kb on + strand, within RPSI07_RS24325 at 959.672 kb on + strand, within RPSI07_RS24325 at 959.673 kb on - strand, within RPSI07_RS24325 at 959.673 kb on - strand, within RPSI07_RS24325 at 959.673 kb on - strand, within RPSI07_RS24325 at 959.673 kb on - strand, within RPSI07_RS24325 at 959.826 kb on - strand, within RPSI07_RS24325
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet remove 957,142 + RPSI07_RS03515 0.88 +0.4 957,142 + RPSI07_RS03515 0.88 -0.5 957,143 - RPSI07_RS03515 0.88 -0.9 957,154 - RPSI07_RS03515 0.89 +2.7 957,284 - -1.4 957,284 - -0.3 957,425 + -1.7 958,059 + +0.2 958,059 + -0.2 958,059 + -0.8 958,060 - +0.0 958,060 - -0.8 958,060 - -0.4 958,060 - +0.5 958,066 + -0.6 958,075 + -1.2 958,075 + -2.6 958,075 + -0.9 958,075 + -0.2 958,075 + -2.0 958,075 + -1.4 958,076 - +0.2 958,076 - -0.9 958,076 - -0.2 958,076 - -1.8 958,076 - +1.2 958,076 - +0.5 958,076 - +1.2 958,076 - -1.4 958,076 - -0.1 958,076 - -0.8 958,122 + +1.7 958,124 - -1.4 958,130 - +0.3 958,156 + +0.1 958,156 + -1.8 958,157 - +0.7 958,180 + RPSI07_RS03525 0.13 -0.0 958,180 + RPSI07_RS03525 0.13 -0.4 958,180 + RPSI07_RS03525 0.13 -0.7 958,181 - RPSI07_RS03525 0.13 +0.2 958,181 - RPSI07_RS03525 0.13 +0.5 958,181 - RPSI07_RS03525 0.13 +0.3 958,303 - RPSI07_RS03525 0.28 -0.2 958,303 - RPSI07_RS03525 0.28 -1.1 958,312 + RPSI07_RS03525 0.29 -0.2 958,486 + RPSI07_RS03525 0.51 -1.2 958,486 + RPSI07_RS03525 0.51 -0.8 958,486 + RPSI07_RS03525 0.51 -0.4 958,486 + RPSI07_RS03525 0.51 -0.8 958,487 - RPSI07_RS03525 0.51 +0.6 958,487 - RPSI07_RS03525 0.51 -1.0 958,487 - RPSI07_RS03525 0.51 -1.4 958,487 - RPSI07_RS03525 0.51 +0.4 958,574 + RPSI07_RS03525 0.62 -0.0 958,575 - RPSI07_RS03525 0.63 -0.1 958,672 + RPSI07_RS03525 0.75 +1.0 958,672 + RPSI07_RS03525 0.75 -1.8 958,672 + RPSI07_RS03525 0.75 -1.2 958,673 - RPSI07_RS03525 0.75 +1.6 958,673 - RPSI07_RS03525 0.75 -0.9 958,673 - RPSI07_RS03525 0.75 +0.4 958,673 - RPSI07_RS03525 0.75 +1.0 958,673 - RPSI07_RS03525 0.75 +2.3 958,673 - RPSI07_RS03525 0.75 -0.7 958,717 + RPSI07_RS03525 0.81 -1.2 958,717 + RPSI07_RS03525 0.81 -0.6 958,718 - RPSI07_RS03525 0.81 +0.4 958,718 - RPSI07_RS03525 0.81 -0.5 958,752 + RPSI07_RS03525 0.85 +0.6 958,753 - RPSI07_RS03525 0.85 +1.4 958,755 - RPSI07_RS03525 0.85 -0.6 958,759 + RPSI07_RS03525 0.86 -0.2 958,951 + -0.8 958,952 + -1.0 958,952 + +0.1 958,952 + -0.1 958,952 + -2.2 958,952 + -0.5 958,952 + -0.1 958,952 + +0.4 958,952 + -0.9 958,953 - +2.0 958,953 - -0.8 958,953 - +0.3 958,953 - +0.3 958,953 - +0.4 958,953 - -1.9 958,953 - -1.0 958,953 - -0.4 958,953 - -1.8 958,953 - +0.6 958,953 - -2.3 958,953 - +0.1 958,953 - +0.6 958,953 - +1.7 958,953 - +2.2 958,953 - +0.5 958,953 - +0.1 958,953 - -0.7 959,097 + +0.4 959,097 + +0.3 959,097 + -0.3 959,098 - +2.2 959,098 - +0.2 959,098 - +0.4 959,098 - -1.0 959,098 - -0.3 959,098 - +0.6 959,513 + RPSI07_RS24325 0.43 -2.0 959,513 + RPSI07_RS24325 0.43 -0.0 959,513 + RPSI07_RS24325 0.43 -0.7 959,672 + RPSI07_RS24325 0.65 -0.3 959,673 - RPSI07_RS24325 0.65 -0.6 959,673 - RPSI07_RS24325 0.65 -0.6 959,673 - RPSI07_RS24325 0.65 +0.1 959,673 - RPSI07_RS24325 0.65 -1.0 959,826 - RPSI07_RS24325 0.86 -1.8
Or see this region's nucleotide sequence