Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS02635 and RPSI07_RS02640 are separated by 282 nucleotides RPSI07_RS02640 and RPSI07_RS02645 are separated by 11 nucleotides RPSI07_RS02645 and RPSI07_RS02650 are separated by 69 nucleotides
RPSI07_RS02635: RPSI07_RS02635 - RNA ligase RtcB family protein, at 746,378 to 747,514
_RS02635
RPSI07_RS02640: RPSI07_RS02640 - DUF2783 domain-containing protein, at 747,797 to 748,003
_RS02640
RPSI07_RS02645: RPSI07_RS02645 - FAD-dependent oxidoreductase, at 748,015 to 749,694
_RS02645
RPSI07_RS02650: RPSI07_RS02650 - MBL fold metallo-hydrolase, at 749,764 to 750,723
_RS02650
Position (kb)
748
749
750 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 747.072 kb on + strand, within RPSI07_RS02635 at 747.072 kb on + strand, within RPSI07_RS02635 at 747.073 kb on - strand, within RPSI07_RS02635 at 747.073 kb on - strand, within RPSI07_RS02635 at 747.138 kb on + strand, within RPSI07_RS02635 at 747.138 kb on + strand, within RPSI07_RS02635 at 747.139 kb on - strand, within RPSI07_RS02635 at 747.139 kb on - strand, within RPSI07_RS02635 at 747.139 kb on - strand, within RPSI07_RS02635 at 747.139 kb on - strand, within RPSI07_RS02635 at 747.139 kb on - strand, within RPSI07_RS02635 at 747.170 kb on - strand, within RPSI07_RS02635 at 747.525 kb on + strand at 747.592 kb on + strand at 747.670 kb on + strand at 747.671 kb on - strand at 747.704 kb on + strand at 747.705 kb on - strand at 747.705 kb on - strand at 747.707 kb on + strand at 747.707 kb on + strand at 747.707 kb on + strand at 747.707 kb on + strand at 747.708 kb on - strand at 747.708 kb on - strand at 747.708 kb on - strand at 747.708 kb on - strand at 747.708 kb on - strand at 747.779 kb on + strand at 747.869 kb on - strand, within RPSI07_RS02640 at 747.869 kb on - strand, within RPSI07_RS02640 at 747.870 kb on - strand, within RPSI07_RS02640 at 747.958 kb on - strand, within RPSI07_RS02640 at 748.148 kb on + strand at 748.148 kb on + strand at 748.148 kb on + strand at 748.148 kb on + strand at 748.149 kb on - strand at 748.149 kb on - strand at 748.149 kb on - strand at 748.149 kb on - strand at 748.149 kb on - strand at 748.640 kb on + strand, within RPSI07_RS02645 at 748.640 kb on + strand, within RPSI07_RS02645 at 748.640 kb on + strand, within RPSI07_RS02645 at 748.641 kb on - strand, within RPSI07_RS02645 at 748.641 kb on - strand, within RPSI07_RS02645 at 748.828 kb on - strand, within RPSI07_RS02645 at 748.828 kb on - strand, within RPSI07_RS02645 at 748.829 kb on + strand, within RPSI07_RS02645 at 748.829 kb on + strand, within RPSI07_RS02645 at 748.829 kb on + strand, within RPSI07_RS02645 at 748.829 kb on + strand, within RPSI07_RS02645 at 748.830 kb on - strand, within RPSI07_RS02645 at 748.830 kb on - strand, within RPSI07_RS02645 at 748.830 kb on - strand, within RPSI07_RS02645 at 749.159 kb on + strand, within RPSI07_RS02645 at 749.159 kb on + strand, within RPSI07_RS02645 at 749.159 kb on + strand, within RPSI07_RS02645 at 749.178 kb on - strand, within RPSI07_RS02645 at 749.309 kb on + strand, within RPSI07_RS02645 at 749.309 kb on + strand, within RPSI07_RS02645 at 749.309 kb on + strand, within RPSI07_RS02645 at 749.309 kb on + strand, within RPSI07_RS02645 at 749.309 kb on + strand, within RPSI07_RS02645 at 749.309 kb on + strand, within RPSI07_RS02645 at 749.310 kb on - strand, within RPSI07_RS02645 at 749.310 kb on - strand, within RPSI07_RS02645 at 749.310 kb on - strand, within RPSI07_RS02645 at 749.312 kb on + strand, within RPSI07_RS02645 at 749.312 kb on + strand, within RPSI07_RS02645 at 749.312 kb on + strand, within RPSI07_RS02645 at 749.313 kb on - strand, within RPSI07_RS02645 at 749.314 kb on - strand, within RPSI07_RS02645 at 749.376 kb on - strand, within RPSI07_RS02645 at 749.376 kb on - strand, within RPSI07_RS02645 at 749.660 kb on + strand at 749.661 kb on - strand at 749.682 kb on - strand at 749.846 kb on + strand at 749.882 kb on + strand, within RPSI07_RS02650 at 749.882 kb on + strand, within RPSI07_RS02650 at 749.882 kb on + strand, within RPSI07_RS02650 at 749.883 kb on - strand, within RPSI07_RS02650 at 749.883 kb on - strand, within RPSI07_RS02650 at 750.143 kb on + strand, within RPSI07_RS02650 at 750.144 kb on - strand, within RPSI07_RS02650 at 750.158 kb on + strand, within RPSI07_RS02650 at 750.407 kb on + strand, within RPSI07_RS02650 at 750.407 kb on + strand, within RPSI07_RS02650 at 750.407 kb on + strand, within RPSI07_RS02650 at 750.408 kb on - strand, within RPSI07_RS02650 at 750.453 kb on - strand, within RPSI07_RS02650 at 750.485 kb on + strand, within RPSI07_RS02650 at 750.486 kb on - strand, within RPSI07_RS02650 at 750.641 kb on + strand at 750.641 kb on + strand at 750.641 kb on + strand at 750.641 kb on + strand at 750.641 kb on + strand at 750.641 kb on + strand at 750.641 kb on + strand at 750.642 kb on - strand at 750.642 kb on - strand at 750.642 kb on - strand at 750.642 kb on - strand at 750.642 kb on - strand at 750.642 kb on - strand at 750.647 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet remove 747,072 + RPSI07_RS02635 0.61 -3.2 747,072 + RPSI07_RS02635 0.61 -1.2 747,073 - RPSI07_RS02635 0.61 -1.6 747,073 - RPSI07_RS02635 0.61 -0.9 747,138 + RPSI07_RS02635 0.67 -3.3 747,138 + RPSI07_RS02635 0.67 +1.9 747,139 - RPSI07_RS02635 0.67 -0.8 747,139 - RPSI07_RS02635 0.67 +0.4 747,139 - RPSI07_RS02635 0.67 -0.2 747,139 - RPSI07_RS02635 0.67 -0.9 747,139 - RPSI07_RS02635 0.67 -1.4 747,170 - RPSI07_RS02635 0.70 +0.3 747,525 + +0.2 747,592 + -2.2 747,670 + +0.1 747,671 - -0.1 747,704 + +0.2 747,705 - +2.0 747,705 - -1.4 747,707 + +1.2 747,707 + -1.4 747,707 + -0.4 747,707 + +1.8 747,708 - +0.5 747,708 - +1.0 747,708 - -1.7 747,708 - -1.4 747,708 - +1.2 747,779 + -0.4 747,869 - RPSI07_RS02640 0.35 -2.4 747,869 - RPSI07_RS02640 0.35 +0.9 747,870 - RPSI07_RS02640 0.35 +0.6 747,958 - RPSI07_RS02640 0.78 +0.4 748,148 + -0.2 748,148 + +1.2 748,148 + +0.1 748,148 + +1.2 748,149 - -0.8 748,149 - -0.5 748,149 - -0.5 748,149 - -1.0 748,149 - -1.8 748,640 + RPSI07_RS02645 0.37 -0.1 748,640 + RPSI07_RS02645 0.37 -1.0 748,640 + RPSI07_RS02645 0.37 -1.4 748,641 - RPSI07_RS02645 0.37 +1.2 748,641 - RPSI07_RS02645 0.37 +0.9 748,828 - RPSI07_RS02645 0.48 +0.2 748,828 - RPSI07_RS02645 0.48 -0.8 748,829 + RPSI07_RS02645 0.48 -0.7 748,829 + RPSI07_RS02645 0.48 +1.2 748,829 + RPSI07_RS02645 0.48 +0.5 748,829 + RPSI07_RS02645 0.48 +2.0 748,830 - RPSI07_RS02645 0.49 -1.0 748,830 - RPSI07_RS02645 0.49 +3.0 748,830 - RPSI07_RS02645 0.49 -3.2 749,159 + RPSI07_RS02645 0.68 +1.3 749,159 + RPSI07_RS02645 0.68 -0.1 749,159 + RPSI07_RS02645 0.68 +0.9 749,178 - RPSI07_RS02645 0.69 +0.2 749,309 + RPSI07_RS02645 0.77 +1.2 749,309 + RPSI07_RS02645 0.77 -0.0 749,309 + RPSI07_RS02645 0.77 +0.5 749,309 + RPSI07_RS02645 0.77 +2.2 749,309 + RPSI07_RS02645 0.77 -1.8 749,309 + RPSI07_RS02645 0.77 +2.8 749,310 - RPSI07_RS02645 0.77 -0.7 749,310 - RPSI07_RS02645 0.77 +1.6 749,310 - RPSI07_RS02645 0.77 -0.3 749,312 + RPSI07_RS02645 0.77 -1.1 749,312 + RPSI07_RS02645 0.77 +0.8 749,312 + RPSI07_RS02645 0.77 +0.7 749,313 - RPSI07_RS02645 0.77 -1.9 749,314 - RPSI07_RS02645 0.77 +0.2 749,376 - RPSI07_RS02645 0.81 -2.1 749,376 - RPSI07_RS02645 0.81 +0.8 749,660 + +1.2 749,661 - +0.4 749,682 - +1.1 749,846 + -1.1 749,882 + RPSI07_RS02650 0.12 -0.7 749,882 + RPSI07_RS02650 0.12 -0.8 749,882 + RPSI07_RS02650 0.12 +2.6 749,883 - RPSI07_RS02650 0.12 -0.8 749,883 - RPSI07_RS02650 0.12 -0.3 750,143 + RPSI07_RS02650 0.39 -2.0 750,144 - RPSI07_RS02650 0.40 -0.1 750,158 + RPSI07_RS02650 0.41 -0.2 750,407 + RPSI07_RS02650 0.67 -1.4 750,407 + RPSI07_RS02650 0.67 -0.2 750,407 + RPSI07_RS02650 0.67 -0.1 750,408 - RPSI07_RS02650 0.67 -0.1 750,453 - RPSI07_RS02650 0.72 +0.1 750,485 + RPSI07_RS02650 0.75 -1.5 750,486 - RPSI07_RS02650 0.75 +1.2 750,641 + -1.5 750,641 + +1.8 750,641 + -0.1 750,641 + +2.2 750,641 + -0.8 750,641 + +0.1 750,641 + -0.9 750,642 - +3.2 750,642 - -0.7 750,642 - -1.8 750,642 - +1.2 750,642 - +0.3 750,642 - +1.2 750,647 + -1.6
Or see this region's nucleotide sequence