Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS02465 and RPSI07_RS24275 are separated by 176 nucleotides RPSI07_RS24275 and RPSI07_RS24280 are separated by 10 nucleotides RPSI07_RS24280 and RPSI07_RS02475 are separated by 194 nucleotides RPSI07_RS02475 and RPSI07_RS02480 are separated by 179 nucleotides RPSI07_RS02480 and RPSI07_RS02485 are separated by 19 nucleotides
RPSI07_RS02465: RPSI07_RS02465 - hydrolase, at 706,221 to 706,859
_RS02465
RPSI07_RS24275: RPSI07_RS24275 - hypothetical protein, at 707,036 to 707,388
_RS24275
RPSI07_RS24280: RPSI07_RS24280 - hypothetical protein, at 707,399 to 707,644
_RS24280
RPSI07_RS02475: RPSI07_RS02475 - MFS transporter, at 707,839 to 709,431
_RS02475
RPSI07_RS02480: RPSI07_RS02480 - transcriptional regulator, at 709,611 to 710,087
_RS02480
RPSI07_RS02485: RPSI07_RS02485 - magnesium and cobalt transport protein CorA, at 710,107 to 711,078
_RS02485
Position (kb) 707
708
709
710 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 706.884 kb on - strand at 706.895 kb on + strand at 706.895 kb on + strand at 706.896 kb on - strand at 706.896 kb on - strand at 706.896 kb on - strand at 706.896 kb on - strand at 706.896 kb on - strand at 706.923 kb on + strand at 706.923 kb on + strand at 706.923 kb on + strand at 706.923 kb on + strand at 706.923 kb on + strand at 706.923 kb on + strand at 706.923 kb on + strand at 706.923 kb on + strand at 706.924 kb on - strand at 706.924 kb on - strand at 706.924 kb on - strand at 706.924 kb on - strand at 706.924 kb on - strand at 706.924 kb on - strand at 706.924 kb on - strand at 706.924 kb on - strand at 706.924 kb on - strand at 706.924 kb on - strand at 706.924 kb on - strand at 706.924 kb on - strand at 706.924 kb on - strand at 706.924 kb on - strand at 706.924 kb on - strand at 706.924 kb on - strand at 706.925 kb on + strand at 706.925 kb on + strand at 706.926 kb on - strand at 706.926 kb on - strand at 706.972 kb on + strand at 706.973 kb on + strand at 706.973 kb on + strand at 706.973 kb on + strand at 706.973 kb on + strand at 706.973 kb on + strand at 706.973 kb on + strand at 706.973 kb on + strand at 706.973 kb on + strand at 706.973 kb on + strand at 706.973 kb on + strand at 706.973 kb on + strand at 706.974 kb on - strand at 706.974 kb on - strand at 706.974 kb on - strand at 706.974 kb on - strand at 706.974 kb on - strand at 706.974 kb on - strand at 706.974 kb on - strand at 706.974 kb on - strand at 706.974 kb on - strand at 706.974 kb on - strand at 706.974 kb on - strand at 706.974 kb on - strand at 706.974 kb on - strand at 706.974 kb on - strand at 706.996 kb on + strand at 706.996 kb on + strand at 706.997 kb on - strand at 706.997 kb on - strand at 707.004 kb on + strand at 707.004 kb on + strand at 707.005 kb on - strand at 707.023 kb on + strand at 707.023 kb on + strand at 707.024 kb on - strand at 707.024 kb on - strand at 707.054 kb on + strand at 707.054 kb on + strand at 707.055 kb on - strand at 707.055 kb on - strand at 707.055 kb on - strand at 707.055 kb on - strand at 707.057 kb on + strand at 707.057 kb on + strand at 707.057 kb on + strand at 707.057 kb on + strand at 707.057 kb on + strand at 707.057 kb on + strand at 707.057 kb on + strand at 707.057 kb on + strand at 707.057 kb on + strand at 707.057 kb on + strand at 707.057 kb on + strand at 707.057 kb on + strand at 707.058 kb on - strand at 707.058 kb on - strand at 707.058 kb on - strand at 707.058 kb on - strand at 707.058 kb on - strand at 707.058 kb on - strand at 707.058 kb on - strand at 707.162 kb on + strand, within RPSI07_RS24275 at 707.278 kb on + strand, within RPSI07_RS24275 at 707.360 kb on - strand at 707.360 kb on - strand at 707.360 kb on - strand at 707.360 kb on - strand at 707.386 kb on + strand at 707.386 kb on + strand at 707.387 kb on - strand at 707.387 kb on - strand at 707.387 kb on - strand at 707.596 kb on + strand, within RPSI07_RS24280 at 707.596 kb on + strand, within RPSI07_RS24280 at 707.596 kb on + strand, within RPSI07_RS24280 at 707.596 kb on + strand, within RPSI07_RS24280 at 707.597 kb on - strand, within RPSI07_RS24280 at 707.597 kb on - strand, within RPSI07_RS24280 at 707.597 kb on - strand, within RPSI07_RS24280 at 707.597 kb on - strand, within RPSI07_RS24280 at 707.597 kb on - strand, within RPSI07_RS24280 at 707.619 kb on + strand, within RPSI07_RS24280 at 707.619 kb on + strand, within RPSI07_RS24280 at 707.619 kb on + strand, within RPSI07_RS24280 at 707.729 kb on + strand at 707.729 kb on + strand at 707.729 kb on + strand at 707.729 kb on + strand at 707.729 kb on + strand at 707.729 kb on + strand at 707.729 kb on + strand at 707.730 kb on - strand at 707.730 kb on - strand at 707.730 kb on - strand at 707.730 kb on - strand at 707.730 kb on - strand at 707.746 kb on + strand at 707.746 kb on + strand at 707.746 kb on + strand at 707.746 kb on + strand at 707.747 kb on - strand at 707.789 kb on + strand at 707.795 kb on - strand at 708.010 kb on + strand, within RPSI07_RS02475 at 708.011 kb on - strand, within RPSI07_RS02475 at 708.031 kb on + strand, within RPSI07_RS02475 at 708.031 kb on + strand, within RPSI07_RS02475 at 708.031 kb on + strand, within RPSI07_RS02475 at 708.032 kb on - strand, within RPSI07_RS02475 at 708.032 kb on - strand, within RPSI07_RS02475 at 708.032 kb on - strand, within RPSI07_RS02475 at 708.083 kb on + strand, within RPSI07_RS02475 at 708.085 kb on + strand, within RPSI07_RS02475 at 708.086 kb on - strand, within RPSI07_RS02475 at 708.229 kb on + strand, within RPSI07_RS02475 at 708.229 kb on + strand, within RPSI07_RS02475 at 708.230 kb on - strand, within RPSI07_RS02475 at 708.376 kb on + strand, within RPSI07_RS02475 at 708.376 kb on + strand, within RPSI07_RS02475 at 708.376 kb on + strand, within RPSI07_RS02475 at 708.377 kb on - strand, within RPSI07_RS02475 at 708.377 kb on - strand, within RPSI07_RS02475 at 708.377 kb on - strand, within RPSI07_RS02475 at 708.377 kb on - strand, within RPSI07_RS02475 at 708.730 kb on + strand, within RPSI07_RS02475 at 708.730 kb on + strand, within RPSI07_RS02475 at 708.731 kb on - strand, within RPSI07_RS02475 at 708.731 kb on - strand, within RPSI07_RS02475 at 708.877 kb on + strand, within RPSI07_RS02475 at 708.877 kb on + strand at 708.878 kb on - strand, within RPSI07_RS02475 at 708.878 kb on - strand, within RPSI07_RS02475 at 709.576 kb on - strand at 709.609 kb on + strand at 709.609 kb on + strand at 709.609 kb on + strand at 709.609 kb on + strand at 709.610 kb on - strand at 709.610 kb on - strand at 709.610 kb on - strand at 709.610 kb on - strand at 709.610 kb on - strand at 709.662 kb on + strand, within RPSI07_RS02480 at 709.662 kb on + strand, within RPSI07_RS02480 at 709.662 kb on + strand, within RPSI07_RS02480 at 709.662 kb on + strand, within RPSI07_RS02480 at 709.662 kb on + strand, within RPSI07_RS02480 at 709.662 kb on + strand, within RPSI07_RS02480 at 709.662 kb on + strand, within RPSI07_RS02480 at 709.662 kb on + strand, within RPSI07_RS02480 at 709.662 kb on + strand, within RPSI07_RS02480 at 709.663 kb on - strand, within RPSI07_RS02480 at 709.692 kb on + strand, within RPSI07_RS02480 at 709.692 kb on + strand, within RPSI07_RS02480 at 709.693 kb on - strand, within RPSI07_RS02480 at 709.693 kb on - strand, within RPSI07_RS02480 at 709.693 kb on - strand, within RPSI07_RS02480 at 710.332 kb on + strand, within RPSI07_RS02485 at 710.332 kb on + strand, within RPSI07_RS02485 at 710.332 kb on + strand, within RPSI07_RS02485 at 710.333 kb on - strand, within RPSI07_RS02485 at 710.333 kb on - strand, within RPSI07_RS02485 at 710.333 kb on - strand, within RPSI07_RS02485
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet remove 706,884 - +0.4 706,895 + -1.1 706,895 + -0.3 706,896 - +0.2 706,896 - -0.4 706,896 - +0.8 706,896 - -0.6 706,896 - +0.3 706,923 + -1.5 706,923 + -1.7 706,923 + +2.5 706,923 + +1.9 706,923 + +0.6 706,923 + +1.6 706,923 + -0.3 706,923 + -1.0 706,924 - -0.1 706,924 - -2.3 706,924 - -0.5 706,924 - +0.3 706,924 - -0.4 706,924 - +0.5 706,924 - -3.6 706,924 - +0.2 706,924 - +0.2 706,924 - -0.1 706,924 - -1.7 706,924 - +0.2 706,924 - +0.4 706,924 - -0.6 706,924 - -0.3 706,924 - +0.2 706,925 + -1.2 706,925 + +0.6 706,926 - -0.2 706,926 - +3.8 706,972 + +1.2 706,973 + -0.7 706,973 + -0.3 706,973 + -0.5 706,973 + -0.8 706,973 + -1.0 706,973 + -0.5 706,973 + -0.4 706,973 + -0.7 706,973 + +0.4 706,973 + -0.2 706,973 + +0.1 706,974 - -1.1 706,974 - +1.3 706,974 - +1.1 706,974 - +2.2 706,974 - -0.2 706,974 - -1.1 706,974 - -0.7 706,974 - +1.2 706,974 - +0.2 706,974 - +0.7 706,974 - -0.3 706,974 - +0.2 706,974 - -1.0 706,974 - +0.0 706,996 + +0.6 706,996 + +0.4 706,997 - +0.0 706,997 - -0.8 707,004 + -1.2 707,004 + +0.4 707,005 - -1.3 707,023 + -0.1 707,023 + -1.3 707,024 - +0.8 707,024 - -2.3 707,054 + +1.2 707,054 + +0.6 707,055 - +0.5 707,055 - -2.3 707,055 - +0.3 707,055 - +2.1 707,057 + +4.4 707,057 + +0.4 707,057 + +0.3 707,057 + +0.4 707,057 + -1.5 707,057 + -1.6 707,057 + -1.3 707,057 + -0.5 707,057 + -1.1 707,057 + +0.1 707,057 + +1.2 707,057 + -0.5 707,058 - -0.9 707,058 - +2.2 707,058 - -0.0 707,058 - +0.1 707,058 - -0.5 707,058 - -0.5 707,058 - -0.8 707,162 + RPSI07_RS24275 0.36 -0.3 707,278 + RPSI07_RS24275 0.69 -0.6 707,360 - -1.1 707,360 - +0.1 707,360 - +0.5 707,360 - -0.8 707,386 + -0.9 707,386 + -0.4 707,387 - +0.2 707,387 - -0.8 707,387 - +0.5 707,596 + RPSI07_RS24280 0.80 +0.5 707,596 + RPSI07_RS24280 0.80 +0.8 707,596 + RPSI07_RS24280 0.80 +1.4 707,596 + RPSI07_RS24280 0.80 -0.1 707,597 - RPSI07_RS24280 0.80 -1.1 707,597 - RPSI07_RS24280 0.80 +3.1 707,597 - RPSI07_RS24280 0.80 +2.2 707,597 - RPSI07_RS24280 0.80 +0.9 707,597 - RPSI07_RS24280 0.80 -1.6 707,619 + RPSI07_RS24280 0.89 -1.1 707,619 + RPSI07_RS24280 0.89 +1.2 707,619 + RPSI07_RS24280 0.89 +1.2 707,729 + -0.2 707,729 + -0.4 707,729 + -0.6 707,729 + +0.9 707,729 + +1.0 707,729 + -2.1 707,729 + +1.4 707,730 - +1.2 707,730 - -1.8 707,730 - +0.9 707,730 - +1.4 707,730 - -0.2 707,746 + -0.1 707,746 + -0.9 707,746 + -0.1 707,746 + +4.4 707,747 - -0.6 707,789 + -0.8 707,795 - +0.5 708,010 + RPSI07_RS02475 0.11 +0.1 708,011 - RPSI07_RS02475 0.11 -0.6 708,031 + RPSI07_RS02475 0.12 -0.1 708,031 + RPSI07_RS02475 0.12 +0.3 708,031 + RPSI07_RS02475 0.12 -0.9 708,032 - RPSI07_RS02475 0.12 -1.8 708,032 - RPSI07_RS02475 0.12 -0.1 708,032 - RPSI07_RS02475 0.12 +1.2 708,083 + RPSI07_RS02475 0.15 -0.2 708,085 + RPSI07_RS02475 0.15 -0.8 708,086 - RPSI07_RS02475 0.16 +1.8 708,229 + RPSI07_RS02475 0.24 -0.7 708,229 + RPSI07_RS02475 0.24 -0.3 708,230 - RPSI07_RS02475 0.25 -0.6 708,376 + RPSI07_RS02475 0.34 -0.2 708,376 + RPSI07_RS02475 0.34 +0.4 708,376 + RPSI07_RS02475 0.34 -1.1 708,377 - RPSI07_RS02475 0.34 -0.8 708,377 - RPSI07_RS02475 0.34 +0.2 708,377 - RPSI07_RS02475 0.34 +0.0 708,377 - RPSI07_RS02475 0.34 +0.4 708,730 + RPSI07_RS02475 0.56 -0.5 708,730 + RPSI07_RS02475 0.56 +0.4 708,731 - RPSI07_RS02475 0.56 -1.3 708,731 - RPSI07_RS02475 0.56 -1.3 708,877 + RPSI07_RS02475 0.65 -1.8 708,877 + +1.2 708,878 - RPSI07_RS02475 0.65 -0.4 708,878 - RPSI07_RS02475 0.65 +0.2 709,576 - -0.2 709,609 + -1.4 709,609 + -2.3 709,609 + -1.5 709,609 + -0.5 709,610 - -0.3 709,610 - -1.2 709,610 - -2.8 709,610 - +0.0 709,610 - +1.9 709,662 + RPSI07_RS02480 0.11 -1.1 709,662 + RPSI07_RS02480 0.11 +0.8 709,662 + RPSI07_RS02480 0.11 +0.8 709,662 + RPSI07_RS02480 0.11 -1.2 709,662 + RPSI07_RS02480 0.11 -1.2 709,662 + RPSI07_RS02480 0.11 -2.1 709,662 + RPSI07_RS02480 0.11 -2.5 709,662 + RPSI07_RS02480 0.11 -0.3 709,662 + RPSI07_RS02480 0.11 -0.6 709,663 - RPSI07_RS02480 0.11 -0.9 709,692 + RPSI07_RS02480 0.17 +0.0 709,692 + RPSI07_RS02480 0.17 +1.0 709,693 - RPSI07_RS02480 0.17 -0.4 709,693 - RPSI07_RS02480 0.17 +0.2 709,693 - RPSI07_RS02480 0.17 +0.2 710,332 + RPSI07_RS02485 0.23 -2.1 710,332 + RPSI07_RS02485 0.23 +0.2 710,332 + RPSI07_RS02485 0.23 -1.2 710,333 - RPSI07_RS02485 0.23 -0.5 710,333 - RPSI07_RS02485 0.23 -0.2 710,333 - RPSI07_RS02485 0.23 -0.7
Or see this region's nucleotide sequence