Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS00505 and RPSI07_RS00510 overlap by 14 nucleotides RPSI07_RS00510 and RPSI07_RS00515 are separated by 45 nucleotides RPSI07_RS00515 and RPSI07_RS00520 are separated by 533 nucleotides
RPSI07_RS00505: RPSI07_RS00505 - non-ribosomal peptide synthetase, at 127,696 to 136,674
_RS00505
RPSI07_RS00510: RPSI07_RS00510 - adenylyl-sulfate kinase, at 136,661 to 137,278
_RS00510
RPSI07_RS00515: RPSI07_RS00515 - sulfotransferase, at 137,324 to 138,244
_RS00515
RPSI07_RS00520: RPSI07_RS00520 - hypothetical protein, at 138,778 to 139,413
_RS00520
Position (kb) 137
138
139 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 136.352 kb on + strand at 136.353 kb on - strand at 136.374 kb on - strand at 136.374 kb on - strand at 136.520 kb on + strand at 136.520 kb on + strand at 136.521 kb on - strand at 136.521 kb on - strand at 136.657 kb on - strand at 136.848 kb on + strand, within RPSI07_RS00510 at 136.849 kb on - strand, within RPSI07_RS00510 at 136.928 kb on + strand, within RPSI07_RS00510 at 136.960 kb on - strand, within RPSI07_RS00510 at 137.112 kb on + strand, within RPSI07_RS00510 at 137.112 kb on + strand, within RPSI07_RS00510 at 137.113 kb on - strand, within RPSI07_RS00510 at 137.113 kb on - strand, within RPSI07_RS00510 at 137.113 kb on - strand, within RPSI07_RS00510 at 137.113 kb on - strand, within RPSI07_RS00510 at 137.113 kb on - strand, within RPSI07_RS00510 at 137.142 kb on + strand, within RPSI07_RS00510 at 137.455 kb on + strand, within RPSI07_RS00515 at 137.455 kb on + strand, within RPSI07_RS00515 at 137.540 kb on + strand, within RPSI07_RS00515 at 137.541 kb on - strand, within RPSI07_RS00515 at 137.605 kb on - strand, within RPSI07_RS00515 at 137.655 kb on + strand, within RPSI07_RS00515 at 137.655 kb on + strand, within RPSI07_RS00515 at 137.655 kb on + strand, within RPSI07_RS00515 at 137.655 kb on + strand, within RPSI07_RS00515 at 137.655 kb on + strand, within RPSI07_RS00515 at 137.656 kb on - strand, within RPSI07_RS00515 at 137.656 kb on - strand, within RPSI07_RS00515 at 137.656 kb on - strand, within RPSI07_RS00515 at 137.700 kb on + strand, within RPSI07_RS00515 at 137.701 kb on - strand, within RPSI07_RS00515 at 137.736 kb on + strand, within RPSI07_RS00515 at 138.030 kb on + strand, within RPSI07_RS00515 at 138.030 kb on + strand, within RPSI07_RS00515 at 138.031 kb on - strand, within RPSI07_RS00515 at 138.054 kb on - strand, within RPSI07_RS00515 at 138.307 kb on - strand at 138.338 kb on + strand at 138.338 kb on + strand at 138.338 kb on + strand at 138.338 kb on + strand at 138.338 kb on + strand at 138.339 kb on - strand at 138.339 kb on - strand at 138.339 kb on - strand at 138.339 kb on - strand at 138.339 kb on - strand at 138.339 kb on - strand at 138.339 kb on - strand at 138.446 kb on - strand at 138.642 kb on - strand at 138.648 kb on + strand at 138.648 kb on + strand at 138.648 kb on + strand at 138.649 kb on - strand at 138.649 kb on - strand at 138.649 kb on - strand at 138.649 kb on - strand at 138.649 kb on - strand at 138.715 kb on + strand at 138.715 kb on + strand at 138.716 kb on - strand at 138.716 kb on - strand at 138.716 kb on - strand at 138.716 kb on - strand at 138.716 kb on - strand at 138.716 kb on - strand at 138.892 kb on + strand, within RPSI07_RS00520 at 138.892 kb on + strand, within RPSI07_RS00520 at 138.892 kb on + strand, within RPSI07_RS00520 at 138.893 kb on - strand at 138.952 kb on + strand, within RPSI07_RS00520 at 138.952 kb on + strand, within RPSI07_RS00520 at 138.953 kb on - strand, within RPSI07_RS00520 at 139.018 kb on + strand, within RPSI07_RS00520 at 139.019 kb on - strand, within RPSI07_RS00520 at 139.024 kb on + strand, within RPSI07_RS00520 at 139.087 kb on + strand, within RPSI07_RS00520 at 139.120 kb on + strand, within RPSI07_RS00520 at 139.120 kb on + strand, within RPSI07_RS00520 at 139.120 kb on + strand, within RPSI07_RS00520 at 139.120 kb on + strand, within RPSI07_RS00520 at 139.120 kb on + strand, within RPSI07_RS00520 at 139.120 kb on + strand, within RPSI07_RS00520 at 139.120 kb on + strand, within RPSI07_RS00520 at 139.120 kb on + strand, within RPSI07_RS00520 at 139.121 kb on - strand, within RPSI07_RS00520 at 139.121 kb on - strand, within RPSI07_RS00520 at 139.121 kb on - strand, within RPSI07_RS00520 at 139.121 kb on - strand, within RPSI07_RS00520 at 139.121 kb on - strand, within RPSI07_RS00520 at 139.186 kb on + strand, within RPSI07_RS00520 at 139.186 kb on + strand, within RPSI07_RS00520 at 139.187 kb on - strand, within RPSI07_RS00520 at 139.187 kb on - strand, within RPSI07_RS00520 at 139.187 kb on - strand, within RPSI07_RS00520 at 139.187 kb on - strand, within RPSI07_RS00520
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet remove 136,352 + -1.2 136,353 - +0.0 136,374 - +0.0 136,374 - -1.1 136,520 + +1.8 136,520 + +0.3 136,521 - -1.5 136,521 - -0.7 136,657 - +0.2 136,848 + RPSI07_RS00510 0.30 -0.1 136,849 - RPSI07_RS00510 0.30 +0.3 136,928 + RPSI07_RS00510 0.43 +1.2 136,960 - RPSI07_RS00510 0.48 +0.1 137,112 + RPSI07_RS00510 0.73 -1.0 137,112 + RPSI07_RS00510 0.73 -0.7 137,113 - RPSI07_RS00510 0.73 +2.3 137,113 - RPSI07_RS00510 0.73 -0.4 137,113 - RPSI07_RS00510 0.73 -0.8 137,113 - RPSI07_RS00510 0.73 +0.2 137,113 - RPSI07_RS00510 0.73 +0.3 137,142 + RPSI07_RS00510 0.78 -1.8 137,455 + RPSI07_RS00515 0.14 +0.3 137,455 + RPSI07_RS00515 0.14 -1.4 137,540 + RPSI07_RS00515 0.23 +0.0 137,541 - RPSI07_RS00515 0.24 +0.6 137,605 - RPSI07_RS00515 0.31 -1.2 137,655 + RPSI07_RS00515 0.36 +0.5 137,655 + RPSI07_RS00515 0.36 -0.2 137,655 + RPSI07_RS00515 0.36 +1.3 137,655 + RPSI07_RS00515 0.36 -0.5 137,655 + RPSI07_RS00515 0.36 +0.3 137,656 - RPSI07_RS00515 0.36 -0.8 137,656 - RPSI07_RS00515 0.36 +1.0 137,656 - RPSI07_RS00515 0.36 -0.3 137,700 + RPSI07_RS00515 0.41 +0.9 137,701 - RPSI07_RS00515 0.41 -0.3 137,736 + RPSI07_RS00515 0.45 -1.3 138,030 + RPSI07_RS00515 0.77 -0.8 138,030 + RPSI07_RS00515 0.77 -0.7 138,031 - RPSI07_RS00515 0.77 +0.3 138,054 - RPSI07_RS00515 0.79 -0.2 138,307 - -0.7 138,338 + -2.4 138,338 + -0.4 138,338 + +0.3 138,338 + +1.3 138,338 + -0.5 138,339 - +0.5 138,339 - -0.2 138,339 - -1.6 138,339 - +1.3 138,339 - +1.2 138,339 - -0.7 138,339 - -2.1 138,446 - -0.3 138,642 - +0.1 138,648 + -1.4 138,648 + -1.8 138,648 + -0.4 138,649 - +1.3 138,649 - -0.5 138,649 - -2.0 138,649 - +0.5 138,649 - -0.5 138,715 + +0.1 138,715 + +0.7 138,716 - +4.0 138,716 - -1.1 138,716 - +0.6 138,716 - -0.9 138,716 - -1.0 138,716 - +0.4 138,892 + RPSI07_RS00520 0.18 -0.7 138,892 + RPSI07_RS00520 0.18 -0.8 138,892 + RPSI07_RS00520 0.18 +0.0 138,893 - +1.3 138,952 + RPSI07_RS00520 0.27 -1.6 138,952 + RPSI07_RS00520 0.27 +0.4 138,953 - RPSI07_RS00520 0.28 -1.5 139,018 + RPSI07_RS00520 0.38 -1.7 139,019 - RPSI07_RS00520 0.38 +0.3 139,024 + RPSI07_RS00520 0.39 -1.8 139,087 + RPSI07_RS00520 0.49 -1.5 139,120 + RPSI07_RS00520 0.54 -1.2 139,120 + RPSI07_RS00520 0.54 -1.4 139,120 + RPSI07_RS00520 0.54 +0.2 139,120 + RPSI07_RS00520 0.54 +0.4 139,120 + RPSI07_RS00520 0.54 -1.0 139,120 + RPSI07_RS00520 0.54 -0.2 139,120 + RPSI07_RS00520 0.54 -2.1 139,120 + RPSI07_RS00520 0.54 -0.1 139,121 - RPSI07_RS00520 0.54 -1.7 139,121 - RPSI07_RS00520 0.54 -0.4 139,121 - RPSI07_RS00520 0.54 -1.9 139,121 - RPSI07_RS00520 0.54 +1.0 139,121 - RPSI07_RS00520 0.54 +2.1 139,186 + RPSI07_RS00520 0.64 -1.4 139,186 + RPSI07_RS00520 0.64 +0.2 139,187 - RPSI07_RS00520 0.64 -1.2 139,187 - RPSI07_RS00520 0.64 +0.8 139,187 - RPSI07_RS00520 0.64 -2.4 139,187 - RPSI07_RS00520 0.64 -0.4
Or see this region's nucleotide sequence