Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS06565 and RPSI07_RS06570 are separated by 180 nucleotides RPSI07_RS06570 and RPSI07_RS24635 are separated by 202 nucleotides RPSI07_RS24635 and RPSI07_RS24640 overlap by 51 nucleotides RPSI07_RS24640 and RPSI07_RS06575 are separated by 409 nucleotides
RPSI07_RS06565: RPSI07_RS06565 - class I SAM-dependent methyltransferase, at 1,765,081 to 1,765,713
_RS06565
RPSI07_RS06570: RPSI07_RS06570 - DUF2939 domain-containing protein, at 1,765,894 to 1,766,493
_RS06570
RPSI07_RS24635: RPSI07_RS24635 - hypothetical protein, at 1,766,696 to 1,767,103
_RS24635
RPSI07_RS24640: RPSI07_RS24640 - hypothetical protein, at 1,767,053 to 1,767,445
_RS24640
RPSI07_RS06575: RPSI07_RS06575 - dihydrofolate reductase, at 1,767,855 to 1,768,517
_RS06575
Position (kb)
1766
1767
1768 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5
6 at 1765.774 kb on + strand at 1765.827 kb on + strand at 1765.827 kb on + strand at 1765.827 kb on + strand at 1765.828 kb on - strand at 1765.828 kb on - strand at 1765.828 kb on - strand at 1765.875 kb on + strand at 1765.875 kb on + strand at 1765.889 kb on + strand at 1765.889 kb on + strand at 1765.889 kb on + strand at 1765.889 kb on + strand at 1765.890 kb on - strand at 1765.895 kb on + strand at 1765.895 kb on + strand at 1765.895 kb on + strand at 1765.895 kb on + strand at 1765.895 kb on + strand at 1765.895 kb on + strand at 1765.895 kb on + strand at 1765.895 kb on + strand at 1765.895 kb on + strand at 1765.895 kb on + strand at 1765.895 kb on + strand at 1765.895 kb on + strand at 1765.896 kb on - strand at 1765.896 kb on - strand at 1765.896 kb on - strand at 1765.896 kb on - strand at 1765.896 kb on - strand at 1765.896 kb on - strand at 1765.896 kb on - strand at 1765.896 kb on - strand at 1765.896 kb on - strand at 1765.896 kb on - strand at 1765.896 kb on - strand at 1765.896 kb on - strand at 1765.896 kb on - strand at 1765.896 kb on - strand at 1765.896 kb on - strand at 1765.896 kb on - strand at 1765.915 kb on + strand at 1765.916 kb on - strand at 1765.916 kb on - strand at 1766.022 kb on - strand, within RPSI07_RS06570 at 1766.022 kb on - strand, within RPSI07_RS06570 at 1766.023 kb on - strand, within RPSI07_RS06570 at 1766.027 kb on - strand, within RPSI07_RS06570 at 1766.027 kb on - strand, within RPSI07_RS06570 at 1766.027 kb on - strand, within RPSI07_RS06570 at 1766.033 kb on + strand, within RPSI07_RS06570 at 1766.033 kb on + strand, within RPSI07_RS06570 at 1766.295 kb on + strand, within RPSI07_RS06570 at 1766.295 kb on + strand, within RPSI07_RS06570 at 1766.295 kb on + strand, within RPSI07_RS06570 at 1766.295 kb on + strand, within RPSI07_RS06570 at 1766.295 kb on + strand, within RPSI07_RS06570 at 1766.296 kb on - strand, within RPSI07_RS06570 at 1766.296 kb on - strand, within RPSI07_RS06570 at 1766.296 kb on - strand, within RPSI07_RS06570 at 1766.296 kb on - strand, within RPSI07_RS06570 at 1766.354 kb on + strand, within RPSI07_RS06570 at 1766.354 kb on + strand, within RPSI07_RS06570 at 1766.355 kb on - strand, within RPSI07_RS06570 at 1766.355 kb on - strand, within RPSI07_RS06570 at 1766.355 kb on - strand, within RPSI07_RS06570 at 1766.355 kb on - strand, within RPSI07_RS06570 at 1766.355 kb on - strand, within RPSI07_RS06570 at 1766.417 kb on + strand, within RPSI07_RS06570 at 1766.429 kb on + strand, within RPSI07_RS06570 at 1766.430 kb on - strand, within RPSI07_RS06570 at 1766.430 kb on - strand, within RPSI07_RS06570 at 1766.633 kb on + strand at 1766.633 kb on + strand at 1766.633 kb on + strand at 1766.634 kb on - strand at 1766.634 kb on - strand at 1766.634 kb on - strand at 1766.634 kb on - strand at 1766.634 kb on - strand at 1766.634 kb on - strand at 1766.634 kb on - strand at 1766.634 kb on - strand at 1766.639 kb on - strand at 1766.644 kb on + strand at 1766.644 kb on + strand at 1766.645 kb on - strand at 1766.645 kb on - strand at 1766.645 kb on - strand at 1766.645 kb on - strand at 1766.645 kb on - strand at 1766.647 kb on - strand at 1766.702 kb on + strand at 1766.702 kb on + strand at 1766.702 kb on + strand at 1766.702 kb on + strand at 1766.702 kb on + strand at 1766.703 kb on - strand at 1766.703 kb on - strand at 1766.703 kb on - strand at 1766.703 kb on - strand at 1766.926 kb on + strand, within RPSI07_RS24635 at 1766.927 kb on - strand, within RPSI07_RS24635 at 1766.927 kb on - strand, within RPSI07_RS24635 at 1767.013 kb on + strand, within RPSI07_RS24635 at 1767.013 kb on + strand, within RPSI07_RS24635 at 1767.013 kb on + strand, within RPSI07_RS24635 at 1767.054 kb on + strand at 1767.054 kb on + strand at 1767.054 kb on + strand at 1767.055 kb on - strand at 1767.055 kb on - strand at 1767.055 kb on - strand at 1767.089 kb on + strand at 1767.091 kb on - strand at 1767.106 kb on + strand, within RPSI07_RS24640 at 1767.107 kb on - strand, within RPSI07_RS24640 at 1767.107 kb on - strand, within RPSI07_RS24640 at 1767.140 kb on - strand, within RPSI07_RS24640 at 1767.140 kb on - strand, within RPSI07_RS24640 at 1767.148 kb on - strand, within RPSI07_RS24640 at 1767.148 kb on - strand, within RPSI07_RS24640 at 1767.148 kb on - strand, within RPSI07_RS24640 at 1767.204 kb on - strand, within RPSI07_RS24640 at 1767.210 kb on + strand, within RPSI07_RS24640 at 1767.210 kb on + strand, within RPSI07_RS24640 at 1767.211 kb on - strand, within RPSI07_RS24640 at 1767.211 kb on - strand, within RPSI07_RS24640 at 1767.211 kb on - strand, within RPSI07_RS24640 at 1767.283 kb on + strand, within RPSI07_RS24640 at 1767.283 kb on + strand, within RPSI07_RS24640 at 1767.283 kb on + strand, within RPSI07_RS24640 at 1767.284 kb on - strand, within RPSI07_RS24640 at 1767.284 kb on - strand, within RPSI07_RS24640 at 1767.376 kb on - strand, within RPSI07_RS24640 at 1767.376 kb on - strand, within RPSI07_RS24640 at 1767.377 kb on + strand, within RPSI07_RS24640 at 1767.377 kb on + strand, within RPSI07_RS24640 at 1767.378 kb on - strand, within RPSI07_RS24640 at 1767.378 kb on - strand, within RPSI07_RS24640 at 1767.378 kb on - strand, within RPSI07_RS24640 at 1767.378 kb on - strand, within RPSI07_RS24640 at 1767.436 kb on + strand at 1767.437 kb on - strand at 1767.450 kb on + strand at 1767.451 kb on - strand at 1767.490 kb on - strand at 1767.494 kb on + strand at 1767.494 kb on + strand at 1767.494 kb on + strand at 1767.495 kb on - strand at 1767.495 kb on - strand at 1767.495 kb on - strand at 1767.497 kb on + strand at 1767.497 kb on + strand at 1767.498 kb on - strand at 1767.498 kb on - strand at 1767.498 kb on - strand at 1767.687 kb on - strand at 1767.708 kb on + strand at 1767.708 kb on + strand at 1767.718 kb on + strand at 1767.719 kb on + strand at 1767.719 kb on + strand at 1767.720 kb on - strand at 1767.720 kb on - strand at 1767.720 kb on - strand at 1767.720 kb on - strand at 1767.722 kb on + strand at 1767.723 kb on - strand at 1767.765 kb on + strand at 1767.765 kb on + strand at 1767.790 kb on + strand at 1767.791 kb on - strand at 1767.791 kb on - strand at 1767.793 kb on + strand at 1767.793 kb on + strand at 1767.793 kb on + strand at 1767.793 kb on + strand at 1767.793 kb on + strand at 1767.793 kb on + strand at 1767.794 kb on - strand at 1767.794 kb on - strand at 1767.794 kb on - strand at 1767.794 kb on - strand at 1767.794 kb on - strand at 1767.794 kb on - strand at 1767.824 kb on + strand at 1767.824 kb on + strand at 1767.825 kb on - strand at 1767.825 kb on - strand at 1767.825 kb on - strand at 1767.831 kb on + strand at 1767.832 kb on - strand at 1767.832 kb on - strand at 1767.840 kb on - strand at 1767.971 kb on - strand, within RPSI07_RS06575 at 1767.971 kb on - strand, within RPSI07_RS06575 at 1767.971 kb on - strand, within RPSI07_RS06575 at 1768.020 kb on - strand, within RPSI07_RS06575
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol remove 1,765,774 + +0.8 1,765,827 + -0.4 1,765,827 + -0.4 1,765,827 + -0.8 1,765,828 - -0.2 1,765,828 - -0.4 1,765,828 - -1.2 1,765,875 + -2.5 1,765,875 + +0.5 1,765,889 + -2.3 1,765,889 + -0.6 1,765,889 + +0.2 1,765,889 + +0.5 1,765,890 - -1.6 1,765,895 + -0.3 1,765,895 + -0.1 1,765,895 + -1.3 1,765,895 + -0.4 1,765,895 + -2.2 1,765,895 + +0.7 1,765,895 + +1.2 1,765,895 + -0.8 1,765,895 + -2.5 1,765,895 + +0.3 1,765,895 + +0.9 1,765,895 + -1.6 1,765,896 - +1.2 1,765,896 - -0.5 1,765,896 - -0.8 1,765,896 - +1.2 1,765,896 - +0.3 1,765,896 - -0.3 1,765,896 - +0.2 1,765,896 - -1.6 1,765,896 - +0.6 1,765,896 - +6.5 1,765,896 - -0.0 1,765,896 - +0.1 1,765,896 - -0.4 1,765,896 - -0.4 1,765,896 - -1.8 1,765,896 - -0.6 1,765,915 + -0.5 1,765,916 - +6.3 1,765,916 - +1.2 1,766,022 - RPSI07_RS06570 0.21 -0.2 1,766,022 - RPSI07_RS06570 0.21 +2.0 1,766,023 - RPSI07_RS06570 0.21 +0.8 1,766,027 - RPSI07_RS06570 0.22 +0.3 1,766,027 - RPSI07_RS06570 0.22 -0.6 1,766,027 - RPSI07_RS06570 0.22 -0.3 1,766,033 + RPSI07_RS06570 0.23 +1.3 1,766,033 + RPSI07_RS06570 0.23 -1.4 1,766,295 + RPSI07_RS06570 0.67 -0.8 1,766,295 + RPSI07_RS06570 0.67 -0.5 1,766,295 + RPSI07_RS06570 0.67 +0.2 1,766,295 + RPSI07_RS06570 0.67 +0.3 1,766,295 + RPSI07_RS06570 0.67 +0.3 1,766,296 - RPSI07_RS06570 0.67 -1.3 1,766,296 - RPSI07_RS06570 0.67 -2.3 1,766,296 - RPSI07_RS06570 0.67 +2.0 1,766,296 - RPSI07_RS06570 0.67 +0.8 1,766,354 + RPSI07_RS06570 0.77 +1.8 1,766,354 + RPSI07_RS06570 0.77 +0.8 1,766,355 - RPSI07_RS06570 0.77 -0.0 1,766,355 - RPSI07_RS06570 0.77 +0.8 1,766,355 - RPSI07_RS06570 0.77 -0.5 1,766,355 - RPSI07_RS06570 0.77 +0.0 1,766,355 - RPSI07_RS06570 0.77 +0.5 1,766,417 + RPSI07_RS06570 0.87 +0.1 1,766,429 + RPSI07_RS06570 0.89 -0.5 1,766,430 - RPSI07_RS06570 0.89 -0.3 1,766,430 - RPSI07_RS06570 0.89 +1.7 1,766,633 + -0.4 1,766,633 + +0.2 1,766,633 + -1.2 1,766,634 - +1.0 1,766,634 - -0.9 1,766,634 - -1.4 1,766,634 - -0.4 1,766,634 - +0.3 1,766,634 - -2.3 1,766,634 - -0.2 1,766,634 - -0.3 1,766,639 - -2.2 1,766,644 + +0.8 1,766,644 + +0.1 1,766,645 - -1.0 1,766,645 - +0.8 1,766,645 - +0.3 1,766,645 - -1.2 1,766,645 - +4.6 1,766,647 - -0.8 1,766,702 + -1.6 1,766,702 + -1.5 1,766,702 + -0.8 1,766,702 + -0.6 1,766,702 + -0.4 1,766,703 - +0.8 1,766,703 - +0.1 1,766,703 - -0.8 1,766,703 - +0.2 1,766,926 + RPSI07_RS24635 0.56 -0.5 1,766,927 - RPSI07_RS24635 0.57 -0.6 1,766,927 - RPSI07_RS24635 0.57 -1.8 1,767,013 + RPSI07_RS24635 0.78 -0.5 1,767,013 + RPSI07_RS24635 0.78 -0.4 1,767,013 + RPSI07_RS24635 0.78 -0.9 1,767,054 + +0.6 1,767,054 + +1.2 1,767,054 + -0.4 1,767,055 - +0.0 1,767,055 - +0.0 1,767,055 - -1.0 1,767,089 + +0.2 1,767,091 - -2.2 1,767,106 + RPSI07_RS24640 0.13 +1.0 1,767,107 - RPSI07_RS24640 0.14 -0.6 1,767,107 - RPSI07_RS24640 0.14 -1.3 1,767,140 - RPSI07_RS24640 0.22 +0.8 1,767,140 - RPSI07_RS24640 0.22 +0.0 1,767,148 - RPSI07_RS24640 0.24 -1.0 1,767,148 - RPSI07_RS24640 0.24 -1.5 1,767,148 - RPSI07_RS24640 0.24 -1.0 1,767,204 - RPSI07_RS24640 0.38 -0.4 1,767,210 + RPSI07_RS24640 0.40 -0.2 1,767,210 + RPSI07_RS24640 0.40 -0.9 1,767,211 - RPSI07_RS24640 0.40 +0.3 1,767,211 - RPSI07_RS24640 0.40 -0.2 1,767,211 - RPSI07_RS24640 0.40 -2.8 1,767,283 + RPSI07_RS24640 0.59 +0.3 1,767,283 + RPSI07_RS24640 0.59 -0.7 1,767,283 + RPSI07_RS24640 0.59 -1.7 1,767,284 - RPSI07_RS24640 0.59 +0.8 1,767,284 - RPSI07_RS24640 0.59 +0.1 1,767,376 - RPSI07_RS24640 0.82 -1.5 1,767,376 - RPSI07_RS24640 0.82 -0.9 1,767,377 + RPSI07_RS24640 0.82 -2.9 1,767,377 + RPSI07_RS24640 0.82 -0.1 1,767,378 - RPSI07_RS24640 0.83 -0.3 1,767,378 - RPSI07_RS24640 0.83 +0.7 1,767,378 - RPSI07_RS24640 0.83 -0.1 1,767,378 - RPSI07_RS24640 0.83 -0.5 1,767,436 + -0.5 1,767,437 - +0.4 1,767,450 + -1.4 1,767,451 - -0.2 1,767,490 - +1.4 1,767,494 + +2.6 1,767,494 + -0.7 1,767,494 + -0.2 1,767,495 - +0.0 1,767,495 - +3.2 1,767,495 - +0.1 1,767,497 + +1.1 1,767,497 + +0.2 1,767,498 - +1.5 1,767,498 - +1.0 1,767,498 - -0.4 1,767,687 - +0.6 1,767,708 + -0.5 1,767,708 + -1.8 1,767,718 + -0.4 1,767,719 + -1.5 1,767,719 + +1.4 1,767,720 - -0.2 1,767,720 - +1.6 1,767,720 - +0.7 1,767,720 - +1.8 1,767,722 + -2.1 1,767,723 - -1.3 1,767,765 + -1.7 1,767,765 + -0.4 1,767,790 + +0.3 1,767,791 - -0.1 1,767,791 - +0.5 1,767,793 + +1.1 1,767,793 + -0.6 1,767,793 + -0.3 1,767,793 + +0.0 1,767,793 + -1.2 1,767,793 + -0.4 1,767,794 - +0.2 1,767,794 - -0.5 1,767,794 - -0.9 1,767,794 - +0.2 1,767,794 - +0.7 1,767,794 - -0.1 1,767,824 + +1.2 1,767,824 + -1.0 1,767,825 - -0.2 1,767,825 - +1.1 1,767,825 - -0.2 1,767,831 + +0.8 1,767,832 - -1.4 1,767,832 - +0.6 1,767,840 - +3.5 1,767,971 - RPSI07_RS06575 0.17 -1.6 1,767,971 - RPSI07_RS06575 0.17 -0.4 1,767,971 - RPSI07_RS06575 0.17 +0.8 1,768,020 - RPSI07_RS06575 0.25 +0.2
Or see this region's nucleotide sequence