Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS23565 and RPSI07_RS23570 overlap by 4 nucleotides RPSI07_RS23570 and RPSI07_RS23575 are separated by 13 nucleotides RPSI07_RS23575 and RPSI07_RS23580 are separated by 94 nucleotides
RPSI07_RS23565: RPSI07_RS23565 - PAS domain-containing sensor histidine kinase, at 3,427,748 to 3,430,183
_RS23565
RPSI07_RS23570: RPSI07_RS23570 - DUF4390 domain-containing protein, at 3,430,180 to 3,430,980
_RS23570
RPSI07_RS23575: RPSI07_RS23575 - 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB, at 3,430,994 to 3,432,319
_RS23575
RPSI07_RS23580: RPSI07_RS23580 - zinc metalloprotease HtpX, at 3,432,414 to 3,433,274
_RS23580
Position (kb)
3430
3431
3432
3433 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3430.075 kb on + strand at 3430.164 kb on + strand at 3430.165 kb on - strand at 3430.165 kb on - strand at 3431.020 kb on - strand at 3431.023 kb on - strand at 3431.023 kb on - strand at 3431.124 kb on - strand at 3431.124 kb on - strand at 3431.199 kb on + strand, within RPSI07_RS23575 at 3431.200 kb on - strand, within RPSI07_RS23575 at 3431.200 kb on - strand, within RPSI07_RS23575 at 3431.200 kb on - strand, within RPSI07_RS23575 at 3431.234 kb on + strand, within RPSI07_RS23575 at 3431.235 kb on - strand, within RPSI07_RS23575 at 3431.745 kb on + strand, within RPSI07_RS23575 at 3431.838 kb on + strand, within RPSI07_RS23575 at 3431.874 kb on + strand, within RPSI07_RS23575 at 3431.875 kb on - strand, within RPSI07_RS23575 at 3432.049 kb on + strand, within RPSI07_RS23575 at 3432.165 kb on + strand, within RPSI07_RS23575 at 3432.396 kb on + strand at 3432.397 kb on - strand at 3432.397 kb on - strand at 3432.397 kb on - strand at 3432.397 kb on - strand at 3432.397 kb on - strand at 3432.397 kb on - strand at 3432.398 kb on + strand at 3432.398 kb on + strand at 3432.399 kb on - strand at 3432.399 kb on - strand at 3432.425 kb on - strand at 3432.590 kb on - strand, within RPSI07_RS23580 at 3433.010 kb on - strand, within RPSI07_RS23580 at 3433.063 kb on + strand, within RPSI07_RS23580 at 3433.105 kb on + strand, within RPSI07_RS23580 at 3433.105 kb on + strand, within RPSI07_RS23580 at 3433.105 kb on + strand, within RPSI07_RS23580 at 3433.106 kb on - strand, within RPSI07_RS23580 at 3433.314 kb on + strand at 3433.314 kb on + strand at 3433.314 kb on + strand at 3433.314 kb on + strand at 3433.314 kb on + strand at 3433.315 kb on - strand at 3433.315 kb on - strand at 3433.315 kb on - strand at 3433.315 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol remove 3,430,075 + +0.5 3,430,164 + -1.1 3,430,165 - -1.5 3,430,165 - +1.0 3,431,020 - -1.5 3,431,023 - -0.0 3,431,023 - -0.0 3,431,124 - -0.6 3,431,124 - +0.1 3,431,199 + RPSI07_RS23575 0.15 +1.5 3,431,200 - RPSI07_RS23575 0.16 -1.9 3,431,200 - RPSI07_RS23575 0.16 +1.0 3,431,200 - RPSI07_RS23575 0.16 +0.3 3,431,234 + RPSI07_RS23575 0.18 -1.5 3,431,235 - RPSI07_RS23575 0.18 -2.0 3,431,745 + RPSI07_RS23575 0.57 -1.3 3,431,838 + RPSI07_RS23575 0.64 +2.5 3,431,874 + RPSI07_RS23575 0.66 +2.5 3,431,875 - RPSI07_RS23575 0.66 +0.8 3,432,049 + RPSI07_RS23575 0.80 +0.1 3,432,165 + RPSI07_RS23575 0.88 -0.4 3,432,396 + -1.1 3,432,397 - -0.3 3,432,397 - -0.1 3,432,397 - -2.2 3,432,397 - +0.8 3,432,397 - -0.0 3,432,397 - +0.2 3,432,398 + -1.0 3,432,398 + +0.5 3,432,399 - -0.1 3,432,399 - +1.1 3,432,425 - -2.7 3,432,590 - RPSI07_RS23580 0.20 -1.7 3,433,010 - RPSI07_RS23580 0.69 -0.2 3,433,063 + RPSI07_RS23580 0.75 +1.1 3,433,105 + RPSI07_RS23580 0.80 -1.4 3,433,105 + RPSI07_RS23580 0.80 -0.4 3,433,105 + RPSI07_RS23580 0.80 -0.9 3,433,106 - RPSI07_RS23580 0.80 -1.0 3,433,314 + +0.9 3,433,314 + -1.0 3,433,314 + -0.4 3,433,314 + -0.2 3,433,314 + -0.4 3,433,315 - -2.0 3,433,315 - -0.4 3,433,315 - +1.5 3,433,315 - -1.0
Or see this region's nucleotide sequence