Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS19385 and RPSI07_RS19390 are separated by 280 nucleotides RPSI07_RS19390 and RPSI07_RS19395 are separated by 494 nucleotides
RPSI07_RS19385: RPSI07_RS19385 - patatin, at 2,531,091 to 2,533,424
_RS19385
RPSI07_RS19390: RPSI07_RS19390 - L-lactate permease, at 2,533,705 to 2,535,390
_RS19390
RPSI07_RS19395: RPSI07_RS19395 - multidrug ABC transporter ATP-binding protein, at 2,535,885 to 2,537,716
_RS19395
Position (kb)
2533
2534
2535
2536 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2533.241 kb on - strand at 2533.241 kb on - strand at 2533.425 kb on - strand at 2533.499 kb on + strand at 2533.499 kb on + strand at 2533.572 kb on + strand at 2533.572 kb on + strand at 2533.573 kb on - strand at 2533.573 kb on - strand at 2533.573 kb on - strand at 2533.601 kb on + strand at 2533.601 kb on + strand at 2533.602 kb on - strand at 2533.666 kb on + strand at 2533.721 kb on - strand at 2533.743 kb on - strand at 2534.191 kb on - strand, within RPSI07_RS19390 at 2534.881 kb on + strand, within RPSI07_RS19390 at 2534.882 kb on - strand, within RPSI07_RS19390 at 2534.882 kb on - strand, within RPSI07_RS19390 at 2535.283 kb on + strand at 2535.283 kb on + strand at 2535.283 kb on + strand at 2535.283 kb on + strand at 2535.283 kb on + strand at 2535.284 kb on - strand at 2535.284 kb on - strand at 2535.284 kb on - strand at 2535.346 kb on + strand at 2535.347 kb on - strand at 2535.347 kb on - strand at 2535.388 kb on + strand at 2535.589 kb on + strand at 2535.590 kb on - strand at 2535.590 kb on - strand at 2535.821 kb on + strand at 2535.821 kb on + strand at 2535.821 kb on + strand at 2535.821 kb on + strand at 2535.822 kb on - strand at 2535.822 kb on - strand at 2535.822 kb on - strand at 2535.822 kb on - strand at 2535.822 kb on - strand at 2535.969 kb on + strand at 2535.975 kb on + strand at 2535.976 kb on - strand at 2535.976 kb on - strand at 2536.000 kb on + strand at 2536.000 kb on + strand at 2536.001 kb on - strand at 2536.135 kb on + strand, within RPSI07_RS19395 at 2536.136 kb on - strand, within RPSI07_RS19395 at 2536.257 kb on + strand, within RPSI07_RS19395 at 2536.258 kb on - strand, within RPSI07_RS19395 at 2536.258 kb on - strand, within RPSI07_RS19395 at 2536.258 kb on - strand, within RPSI07_RS19395
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol remove 2,533,241 - -0.6 2,533,241 - +0.3 2,533,425 - -0.6 2,533,499 + -0.8 2,533,499 + -0.6 2,533,572 + +3.3 2,533,572 + -0.4 2,533,573 - +1.8 2,533,573 - +0.2 2,533,573 - +0.3 2,533,601 + -1.0 2,533,601 + +0.4 2,533,602 - -0.7 2,533,666 + -0.3 2,533,721 - -0.6 2,533,743 - -0.3 2,534,191 - RPSI07_RS19390 0.29 +0.2 2,534,881 + RPSI07_RS19390 0.70 -0.2 2,534,882 - RPSI07_RS19390 0.70 +0.6 2,534,882 - RPSI07_RS19390 0.70 -0.4 2,535,283 + -1.7 2,535,283 + +0.6 2,535,283 + -0.6 2,535,283 + -1.7 2,535,283 + -1.4 2,535,284 - +2.3 2,535,284 - -0.3 2,535,284 - -0.7 2,535,346 + +0.2 2,535,347 - +0.2 2,535,347 - -0.5 2,535,388 + +1.0 2,535,589 + +1.2 2,535,590 - +0.0 2,535,590 - -1.4 2,535,821 + -0.6 2,535,821 + -0.9 2,535,821 + -0.3 2,535,821 + +0.7 2,535,822 - +0.7 2,535,822 - -1.1 2,535,822 - -0.4 2,535,822 - -0.9 2,535,822 - +0.4 2,535,969 + -0.6 2,535,975 + -2.3 2,535,976 - +2.2 2,535,976 - -0.3 2,536,000 + +0.7 2,536,000 + -0.0 2,536,001 - +0.5 2,536,135 + RPSI07_RS19395 0.14 -0.3 2,536,136 - RPSI07_RS19395 0.14 -0.3 2,536,257 + RPSI07_RS19395 0.20 -0.2 2,536,258 - RPSI07_RS19395 0.20 -1.2 2,536,258 - RPSI07_RS19395 0.20 +0.1 2,536,258 - RPSI07_RS19395 0.20 -1.3
Or see this region's nucleotide sequence