Strain Fitness in Ralstonia solanacearum PSI07 around RPSI07_RS16665

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRPSI07_RS16660 and RPSI07_RS16665 are separated by 235 nucleotidesRPSI07_RS16665 and RPSI07_RS16670 overlap by 4 nucleotides RPSI07_RS16660: RPSI07_RS16660 - hybrid non-ribosomal peptide synthetase/type I polyketide synthase, at 1,929,654 to 1,942,466 _RS16660 RPSI07_RS16665: RPSI07_RS16665 - thiazolinyl imide reductase, at 1,942,702 to 1,943,865 _RS16665 RPSI07_RS16670: RPSI07_RS16670 - peptide biosynthesis thioesterase, at 1,943,862 to 1,945,589 _RS16670 Position (kb) 1942 1943 1944Strain fitness (log2 ratio) -2 -1 0 1 2 3at 1941.773 kb on - strandat 1941.840 kb on + strandat 1941.910 kb on - strandat 1941.910 kb on - strandat 1942.464 kb on + strandat 1942.464 kb on + strandat 1942.465 kb on - strandat 1942.465 kb on - strandat 1942.465 kb on - strandat 1942.732 kb on + strandat 1942.732 kb on + strandat 1942.732 kb on + strandat 1942.732 kb on + strandat 1942.733 kb on - strandat 1942.733 kb on - strandat 1942.733 kb on - strandat 1942.733 kb on - strandat 1942.733 kb on - strandat 1942.733 kb on - strandat 1942.733 kb on - strandat 1942.733 kb on - strandat 1942.771 kb on + strandat 1942.771 kb on + strandat 1942.772 kb on - strandat 1942.772 kb on - strandat 1942.772 kb on - strandat 1943.030 kb on + strand, within RPSI07_RS16665at 1943.030 kb on + strand, within RPSI07_RS16665at 1943.030 kb on + strand, within RPSI07_RS16665at 1943.030 kb on + strand, within RPSI07_RS16665at 1943.030 kb on + strand, within RPSI07_RS16665at 1943.031 kb on - strand, within RPSI07_RS16665at 1943.031 kb on - strand, within RPSI07_RS16665at 1943.031 kb on - strand, within RPSI07_RS16665at 1943.031 kb on - strand, within RPSI07_RS16665at 1943.031 kb on - strand, within RPSI07_RS16665at 1943.122 kb on + strand, within RPSI07_RS16665at 1943.158 kb on + strand, within RPSI07_RS16665at 1943.158 kb on + strand, within RPSI07_RS16665at 1943.227 kb on + strand, within RPSI07_RS16665at 1943.369 kb on + strand, within RPSI07_RS16665at 1943.369 kb on + strand, within RPSI07_RS16665at 1943.370 kb on - strand, within RPSI07_RS16665at 1943.370 kb on - strand, within RPSI07_RS16665at 1943.396 kb on - strand, within RPSI07_RS16665at 1943.488 kb on + strand, within RPSI07_RS16665at 1943.518 kb on + strand, within RPSI07_RS16665at 1943.518 kb on + strand, within RPSI07_RS16665at 1943.558 kb on - strand, within RPSI07_RS16665at 1943.558 kb on - strand, within RPSI07_RS16665at 1943.846 kb on - strandat 1943.846 kb on - strandat 1943.882 kb on + strandat 1943.883 kb on - strandat 1943.887 kb on + strandat 1943.887 kb on + strandat 1943.887 kb on + strandat 1943.887 kb on + strandat 1943.888 kb on - strandat 1943.888 kb on - strandat 1943.888 kb on - strandat 1943.953 kb on - strandat 1944.007 kb on - strandat 1944.007 kb on - strandat 1944.007 kb on - strandat 1944.633 kb on + strand, within RPSI07_RS16670at 1944.634 kb on - strand, within RPSI07_RS16670at 1944.634 kb on - strand, within RPSI07_RS16670

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol
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1,941,773 - -0.5
1,941,840 + +0.5
1,941,910 - +0.7
1,941,910 - +1.5
1,942,464 + -0.3
1,942,464 + +0.6
1,942,465 - +0.0
1,942,465 - -1.5
1,942,465 - +1.7
1,942,732 + -1.3
1,942,732 + -1.1
1,942,732 + +1.0
1,942,732 + -2.6
1,942,733 - -2.1
1,942,733 - +0.1
1,942,733 - +0.9
1,942,733 - +0.5
1,942,733 - -0.5
1,942,733 - -0.2
1,942,733 - -1.9
1,942,733 - -0.8
1,942,771 + +1.1
1,942,771 + -0.1
1,942,772 - +0.5
1,942,772 - -0.0
1,942,772 - -0.5
1,943,030 + RPSI07_RS16665 0.28 +0.2
1,943,030 + RPSI07_RS16665 0.28 -1.6
1,943,030 + RPSI07_RS16665 0.28 +0.0
1,943,030 + RPSI07_RS16665 0.28 +2.2
1,943,030 + RPSI07_RS16665 0.28 -2.5
1,943,031 - RPSI07_RS16665 0.28 -0.2
1,943,031 - RPSI07_RS16665 0.28 +0.5
1,943,031 - RPSI07_RS16665 0.28 +1.1
1,943,031 - RPSI07_RS16665 0.28 -0.0
1,943,031 - RPSI07_RS16665 0.28 +0.8
1,943,122 + RPSI07_RS16665 0.36 -0.2
1,943,158 + RPSI07_RS16665 0.39 +0.1
1,943,158 + RPSI07_RS16665 0.39 -1.1
1,943,227 + RPSI07_RS16665 0.45 -1.7
1,943,369 + RPSI07_RS16665 0.57 -1.2
1,943,369 + RPSI07_RS16665 0.57 -0.2
1,943,370 - RPSI07_RS16665 0.57 -0.7
1,943,370 - RPSI07_RS16665 0.57 +1.0
1,943,396 - RPSI07_RS16665 0.60 +0.3
1,943,488 + RPSI07_RS16665 0.68 -0.2
1,943,518 + RPSI07_RS16665 0.70 -0.1
1,943,518 + RPSI07_RS16665 0.70 -0.5
1,943,558 - RPSI07_RS16665 0.74 -1.4
1,943,558 - RPSI07_RS16665 0.74 +0.2
1,943,846 - +0.1
1,943,846 - -1.6
1,943,882 + -2.2
1,943,883 - -0.7
1,943,887 + -0.9
1,943,887 + -0.1
1,943,887 + -0.0
1,943,887 + +0.7
1,943,888 - +0.2
1,943,888 - +2.1
1,943,888 - +0.6
1,943,953 - +0.9
1,944,007 - -0.9
1,944,007 - -1.2
1,944,007 - +1.7
1,944,633 + RPSI07_RS16670 0.45 -0.7
1,944,634 - RPSI07_RS16670 0.45 -0.9
1,944,634 - RPSI07_RS16670 0.45 +2.8

Or see this region's nucleotide sequence