Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS13655 and RPSI07_RS13660 are separated by 2 nucleotides RPSI07_RS13660 and RPSI07_RS13665 are separated by 22 nucleotides RPSI07_RS13665 and RPSI07_RS13670 are separated by 52 nucleotides RPSI07_RS13670 and RPSI07_RS13675 overlap by 4 nucleotides
RPSI07_RS13655: RPSI07_RS13655 - septum formation inhibitor Maf, at 1,255,860 to 1,256,477
_RS13655
RPSI07_RS13660: RPSI07_RS13660 - 23S rRNA (pseudouridine(1915)-N(3))-methyltransferase RlmH, at 1,256,480 to 1,256,950
_RS13660
RPSI07_RS13665: RPSI07_RS13665 - ribosome silencing factor, at 1,256,973 to 1,257,569
_RS13665
RPSI07_RS13670: RPSI07_RS13670 - nicotinate-nucleotide adenylyltransferase, at 1,257,622 to 1,258,317
_RS13670
RPSI07_RS13675: RPSI07_RS13675 - oxygen-dependent coproporphyrinogen oxidase, at 1,258,314 to 1,259,222
_RS13675
Position (kb) 1256
1257
1258 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 1256.020 kb on + strand, within RPSI07_RS13655 at 1256.020 kb on + strand, within RPSI07_RS13655 at 1256.314 kb on + strand, within RPSI07_RS13655 at 1256.315 kb on - strand, within RPSI07_RS13655 at 1256.315 kb on - strand, within RPSI07_RS13655 at 1256.449 kb on + strand at 1256.450 kb on - strand at 1256.450 kb on - strand at 1256.884 kb on - strand, within RPSI07_RS13660 at 1256.884 kb on - strand, within RPSI07_RS13660 at 1256.884 kb on - strand, within RPSI07_RS13660 at 1256.884 kb on - strand, within RPSI07_RS13660 at 1257.193 kb on + strand, within RPSI07_RS13665 at 1257.193 kb on + strand, within RPSI07_RS13665 at 1257.278 kb on - strand, within RPSI07_RS13665 at 1257.278 kb on - strand, within RPSI07_RS13665 at 1257.278 kb on - strand, within RPSI07_RS13665 at 1257.280 kb on + strand, within RPSI07_RS13665 at 1257.444 kb on - strand, within RPSI07_RS13665 at 1257.552 kb on + strand at 1257.563 kb on + strand at 1257.569 kb on + strand at 1257.569 kb on + strand at 1257.570 kb on - strand at 1257.570 kb on - strand at 1257.654 kb on - strand at 1258.034 kb on + strand, within RPSI07_RS13670 at 1258.035 kb on - strand, within RPSI07_RS13670 at 1258.317 kb on + strand at 1258.318 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol remove 1,256,020 + RPSI07_RS13655 0.26 -0.3 1,256,020 + RPSI07_RS13655 0.26 +1.7 1,256,314 + RPSI07_RS13655 0.73 +0.4 1,256,315 - RPSI07_RS13655 0.74 -0.4 1,256,315 - RPSI07_RS13655 0.74 -0.1 1,256,449 + +1.2 1,256,450 - -1.2 1,256,450 - +0.1 1,256,884 - RPSI07_RS13660 0.86 -1.1 1,256,884 - RPSI07_RS13660 0.86 -2.0 1,256,884 - RPSI07_RS13660 0.86 -0.9 1,256,884 - RPSI07_RS13660 0.86 -0.1 1,257,193 + RPSI07_RS13665 0.37 -2.3 1,257,193 + RPSI07_RS13665 0.37 -0.9 1,257,278 - RPSI07_RS13665 0.51 +1.8 1,257,278 - RPSI07_RS13665 0.51 +0.5 1,257,278 - RPSI07_RS13665 0.51 -2.5 1,257,280 + RPSI07_RS13665 0.51 -0.1 1,257,444 - RPSI07_RS13665 0.79 +1.2 1,257,552 + +1.5 1,257,563 + +1.4 1,257,569 + +2.5 1,257,569 + -1.2 1,257,570 - -2.4 1,257,570 - +2.4 1,257,654 - -0.6 1,258,034 + RPSI07_RS13670 0.59 -1.6 1,258,035 - RPSI07_RS13670 0.59 -3.2 1,258,317 + -1.0 1,258,318 - -0.2
Or see this region's nucleotide sequence